31results about How to "Improve tissue culture efficiency" patented technology

The invention discloses a rapid tissue culture method for lycium ruthenicum murr, belonging to the technical field of plant tissue culture. The rapid tissue culture method comprises the following steps: explants sterilization, induction culture, rooting culture, subculture, acclimatization and transplantation. According to the rapid tissue culture method, the rooting culture can be carried out during the subculture, so that the tissue culture is high, the operation is simple and convenient, the period is short, and complete plants can be rapidly acquired; the tissue culture process is not influenced by environmental factors.

The invention discloses a method for propagating dendrobium chrysotoxumLindl. seeds by tissue culture, which comprises the following steps: seeding the dendrobium chrysotoxumLindl. seeds into an induction medium for induced germination; then successively transferring the dendrobium chrysotoxumLindl. budlets to a subculture medium and a rooting medium for subsequent cultivation so as to obtain the dendrobium chrysotoxumLindl. rooting tissue cultured seedlings; and then transplanting the dendrobium chrysotoxumLindl. rooting tissue cultured seedlings to greenhouses for seedling hardening. According to the tissue culture method disclosed by the invention, the steps are simple, the operation is convenient, the obtained dendrobium chrysotoxumLindl. rooting tissue cultured seedlings are strong; the growing ability is high, the dendrobium chrysotoxumLindl. rooting tissue cultured seedlings are suitable for industrial production.

The invention discloses a method for establishing a rapid polygonatum sibiricum reproduction system. The method comprises the following steps: seeding terminal buds or tubers of polygonatum sibiricum into an induction medium to induce germination, transferring small polygonatum sibiricum buds into a subculture medium and a rooting medium for continuous culture in sequence, thereby obtaining tissue culture seedlings of polygonatum sibiricum, and transplanting the tissue culture seedlings into a seedbed for seedling hardening. The tissue culture method disclosed by the invention is simple and convenient, simple in step and convenient to operate, obtained polygonatum sibiricum seedlings are strong in plant, high in growing ability and applicable to large-scale production, meanwhile the culture time is effectively shortened, and the production cost is lowered.

The present invention relates to a culture medium for inducing sweet potato clustered shoots belonging to the sweet potato tissue culture technology. The culture medium is formed by dissolving the kinetin KT, 6-benzyl purine 6-BA, heteroauxing IAA, hydrolyzed casein CH, cane sugar and agar into the basic culture medium MS; the quantity of every part is that MS 11, 1.0-2.0 mg of KT, 1.0-2.0 mg of 6-BA, 0.2-2.0mg of IAA, CH 200mg, 20g of cane sugar and 6g of agar. The active effects of the present invention are that a stem tip meristematic tissue is utilized to induce 8.6 shoots and the inducement rate is 860 percent, which obviously promotes the tissue culture efficiency.

The invention provides a polygonatum cyrtonema immature embryo culture micro-stem tuber breeding method. The method comprises the following steps: selection of excellent single plant of polygonatum cyrtonema, stripping of immature seed immature embryos, induction culture of immature embryos, induction formation of micro stem tuber, propagation of micro stem tuber, and direct transplanting of micro stem tuber for seedling establishment. The polygonatum cyrtonema immature embryo culture has the advantages of rapid growth, strong differentiation force, and large propagation coefficient, and propagation and transplanting of the micro stem tuber have the advantages of fast propagation speed, large quantity, short period, convenient transplanting, storage resistance, transport resistance, direct transplanting, simple management and high survival rate; so that the seedling growing problems of slow breeding and seeding formation of the polygonatum cyrtonema seeds, low rhizome propagation coefficient and high cost in the prior art can be solved, polygonatum cyrtonema seedling can be rapidly breed, and the method is suitable for industrial large-scale production.

The invention discloses a tissue culture medium for directly growing a seedling from a barley embryo. The tissue culture medium comprises a seedling growing culture medium and a root growing and seedling strengthening culture medium. A preparation method of the seedling growing culture medium comprises the following steps: firstly, regulating the pH value of every 1L of mother solution of the seedling growing culture medium to be 5.7-5.9; secondly, adding plant gel; thirdly, performing sterilization; and finally, adding filtered and sterilized vitamin B1, vitamin B6, nicotinic acid, 6-benzylaminopurine and 2,4-dichlorphenoxyacetic acid. A preparation method of the root growing and seedling strengthening culture medium comprises the following steps: firstly, adding inositol, casein hydrolysate, proline and malt sugar into every 1L of MS basic culture solution; secondly, regulating the pH value to be 5.8-6.0, then adding the plant gel and then performing the sterilization; and finally, adding the filtered and sterilized vitamin B1. The invention also provides a tissue culture method for directly growing the seedling from the barley embryo, and in the method, tissue culture medium is used.

The invention discloses a mature barley embryo in-vitro culture medium comprising a callus inducing culture medium, a differential medium and a rooting and strengthening culture medium. The invention further discloses a mature barley embryo tissue culture method for inhibiting sprout and rooting effectively by using the mature barley embryo in-vitro culture medium, wherein the mature barley embryo tissue culture method comprises the following steps: firstly, an embryo is scraped as an in-vitro embryo so as to be inoculated on the callus inducing culture medium after seeds are disinfected; secondly, the in-vitro embryo inoculated on the callus inducing culture medium is put in a culture box at the temperature of 22 to 26 DEG C for culture in the dark; thirdly, the obtained callus is transferred to the differential medium and is subjected to illumination culture at the temperature of 22 to 26 DEG C until 2 to 3 leaves are grown; fourthly, the green plant is transferred to the rooting and strengthening culture medium for illumination culture at the temperature of 22 to 26 DEG C until roots of more than or equal to 2 cm are grown; and fifthly, the green plant is placed indoor for 1-2 days, the culture medium on the roots is washed, and then a plant capable of being transplanted is obtained.

The invention relates to a culture medium for inducing sweet potato cluster buds, belonging to a sweet potato tissue culture technology. The culture medium is formed by dissolving kinetin (KT), 6-benzyl purine (6-BA), indoleacetic acid (IAA), casein hydrolysate (CH), cane sugar and agar into a basic culture medium (MS), wherein the dosage of all the components is as follows: 1L of MS, 1.0-2.0mg of KT, 1.0-2.0mg of 6-BA, 0.2-2.0mg of IAA, 200mg of CH, 20g of cane sugar and 6g of agar. The culture medium has the positive effects that after the culture medium is utilized, 8.6 buds can be induced by one stem apical meristem of a sweet potato, so that the induction efficiency is 860%, and the tissue culture efficiency is remarkably improved.

The invention discloses a culture medium for tissue culture of valeriana jatamansi and a culture method. The culture medium for the tissue culture has the advantages of simple components, low cost andeasiness for obtaining; the induction rate, multiplication coefficient and rooting rate of the valeriana jatamansi can be remarkably improved; the tissue culture efficiency can be effectively improved and the production method is reduced; the culture medium has market-oriented application value.

The invention belongs to the technical field of plant tissue culture and relates to a plant tissue culture method using a culture dish. The plant tissue culture method comprises five stages: the first stage is a stage at which aseptic treatment is performed and a culture solution enters a box body: pouring a water absorbing substrate into a tray with holes; the second stage is an aseptic culture stage: opening a front cover, and putting plant tissue materials on the water-absorbing substrate for culturing; the third stage is a stage of changing the culture liquid: opening a liquid leaking hole of the culture dish for draining the culture solution, closing the liquid leaking hole, opening the front cover, then pouring a new culture solution, and closing the cover; the fourth stage is a seedling establishing and rooting stage: cutting out seedlings grown into strains, changing into a rooting culture solution, replacing the tray with the holes with a fixed tray, and putting the seedlings grown into the strains into fixed sleeves; the fifth stage is a seedling hardening stage: opening a gland and an adjustable gland, and transplanting or transporting after about 3 days. Compared with a traditional plant culture method, the method disclosed by the invention has the advantages that cultured plants are shorter in period, and the seedlings are vigorous and high in survival rate.

The invention discloses a scindapsus aureus cluster bud induced culture medium. The scindapsus aureus cluster bud induced culture medium is prepared from an MS basic culture medium, 6-benzylamino adenine, indolebutyric acid, naphthylacetic acid, sucrose, mashed banana and agar powder. According to the scindapsus aureus cluster bud induced culture medium and a scindapsus aureus cluster bud culture method, disclosed by the invention, scindapsus aureus cluster buds are induced by utilizing calluses of scindapsus aureus to obtain a lot of homogeneous clones; the MS culture medium is a basic culture medium, and the 6-benzylamino adenine, the indolebutyric acid, the naphthylacetic acid plant growth regulator, which have specific concentrations, and the mashed banana, the sucrose and the agar powder are combined, so that the cluster bud induced culture medium can be effectively induced. The cluster bud induced culture medium is used for carrying out cluster bud culture on the calluses of the scindapsus aureus, and the induction efficiency can reach 92 percent or more; the reproduction coefficient of scindapsus aureus cluster buds is improved and the tissue culture efficiency is remarkably improved; scindapsus aureus tissue culture seedlings are easily industrially produced.

The invention discloses an efficient tissue culture method of Cattleya hybrida, comprising: sterilizing explants; preventing browning, and performing primary culture, callus induction and subculture; rooting and strengthening, to be specific, culturing with 0.1 mg / L of MS+NAA, 2.0 mg / L of 6-BA, 10 g / L of banana, 100 g / L of mashed potato, 30 g / L of sucrose, 1 g / L of activated carbon and 4 g / L of agar under pH of 5.7 at the temperature of about 23-27 DEG C, and exposing to light under the intensity of 60 Mumol.m<-2>s<-1> for 12 hours per day. The Cattleya hybrida callus induction and proliferation system is optimized, and factory tissue culture efficiency of Cattleya hybrida is improved.

The invention discloses a pinus sylvestris tissue culture medium and a rooting method. The rooting method comprises the following steps: selecting a tender needle leaf part of pinus sylvestris as an explant, and performing sterilization and disinfection treatment; soaking the explant into a NaCl solution for pretreatment before induction; inducing calluses; performing proliferation of the calluses; and performing rooting culture. By adopting the method for inducing and culturing the pinus sylvestris callus, the problems of high difficulty and low efficiency of an existing pinus sylvestris tissue culture technology are solved. The invention solves the problem of selection of tissue culture explants of pinus sylvestris plants, and provides a tissue culture method for induced differentiation,proliferation and rooting. The pinus sylvestris tissue culture medium and the rooting method are of great significance to breeding of pinus sylvestris and other coniferous plants, and have great ecological and economic significance.

The present invention proposes a method for disinfecting seed embryos used in bamboo tissue culture, comprising the following steps: (1) peeling off the lemma of bamboo seed embryos; (2) soaking the seed embryos at a constant temperature of 28 to 32°C for 30 to 48 hours, and changing the water during 6 hours 1 time; (3) Rinse with running water for 1-3 hours; (4) In the ultra-clean workbench, first soak the seed embryos with alcohol, then rinse with sterile water 4-6 times, and then disinfect with 0.8-1.6% formaldehyde solution for 3 ~5h, (5) Dissection: in the ultra-clean workbench, cut off the tail of the bamboo germ embryo with awn. The disinfection method proposed by the present invention is simple to operate, the core step uses formaldehyde solution with relatively low toxicity, and solves the difficult problem of endophytic bacteria pollution in the process of bamboo embryo tissue culture, greatly reduces the pollution rate, and greatly promotes the Tissue culture of bamboo seed embryos. The invention lays a technical foundation for raising seedlings of Phyllostachys pubescens, establishing a callus induction regeneration system of molecular breeding, and the like.

The invention discloses a method for rapid propagation by tissue culture of ox gall bladder, comprising (1) explant selection, (2) explant disinfection, (3) cluster bud induction, (4) cluster bud proliferation, and (5) cluster bud proliferation Rooting series of steps. The invention establishes a high-efficiency and low-cost tissue culture and rapid propagation system of ox bile, which makes the large-scale cultivation of ox bile possible.

The invention discloses a mature barley embryo in-vitro culture medium comprising a callus inducing culture medium, a differential medium and a rooting and strengthening culture medium. The invention further discloses a mature barley embryo tissue culture method for inhibiting sprout and rooting effectively by using the mature barley embryo in-vitro culture medium, wherein the mature barley embryo tissue culture method comprises the following steps: firstly, an embryo is scraped as an in-vitro embryo so as to be inoculated on the callus inducing culture medium after seeds are disinfected; secondly, the in-vitro embryo inoculated on the callus inducing culture medium is put in a culture box at the temperature of 22 to 26 DEG C for culture in the dark; thirdly, the obtained callus is transferred to the differential medium and is subjected to illumination culture at the temperature of 22 to 26 DEG C until 2 to 3 leaves are grown; fourthly, the green plant is transferred to the rooting and strengthening culture medium for illumination culture at the temperature of 22 to 26 DEG C until roots of more than or equal to 2 cm are grown; and fifthly, the green plant is placed indoor for 1-2 days, the culture medium on the roots is washed, and then a plant capable of being transplanted is obtained.

The invention discloses a method for tissue culture and propagation of sundew spoon leaf, which comprises (1) explant selection, (2) explant disinfection, (3) cluster bud induction, (4) cluster bud proliferation, (5) A series of steps for clustering shoots and rooting. The tissue culture propagation method of the invention significantly improves the induction rate, multiplication coefficient and rooting rate of the sundew spoon-leaf sundew, has high tissue culture efficiency, low cost, and has market application value.

The invention discloses a method for preparing a medium for direct seedling growth of Zizania zizania seed embryos. Take 1LMS basic culture solution and adjust the pH value to 5.7-5.9, then add 2.5-4.2g plant gel and 28-32g sucrose, and sterilize at high temperature When the temperature of the sterilized solution drops to 50-60° C., add 2.0 mg of 6-BA mother solution, 2.0 mg of KT mother solution, and 0.2 mg of NAA mother solution to obtain a seedling growth medium. The invention also discloses a method for direct seedling tissue culture of Zizania zizania seed embryos: selecting Zizania zizania seeds for disinfection and sterilization to obtain the seed embryos; inoculating the seed embryos on the seedling growth medium, and performing light irradiation at a temperature of 22-27°C For cultivation, the light intensity is 40‑60 μmol / m 2 / s, the daily light time is 16 hours, until 2 to 3 leaves grow, and green seedlings are obtained, which can significantly reduce the time from mature seeds to seedlings of Zizania zalea.

The invention discloses an open-type culture method for apple dwarfing stock tissue culture seedlings at the rooting stage and a culture medium adopted by the method, and belongs to the technical field of plant tissue culture. The culture method comprises the steps of (1) selecting apple dwarfing stock P3 tissue culture seedlings and apple dwarfing stock MARK tissue culture seedlings; (2) transferring the apple dwarfing stock P3 tissue culture seedlings and the apple dwarfing stock MARK tissue culture seedlings both of which are selected in step (1) into a rooting culture medium containing 0.01-0.015% of Shenxian water for rooting culture, wherein the culture temperature is 25 + / - 2 DEG C, and the illumination time is 12 hours per day, and the rooting culture medium is formed by adding 2.0mg / L IBA, 30 g / L sugar and 6 g / L agar into 1 / 2 MS and not sterilized at high temperature. The method has the following advantages that by adding the bacteriostatic agent Shenxian water into the rooting culture medium, the normal growth of the tissue culture seedlings is ensured, the functions of sterilization and bacterial resistance are achieved, and no harm can be caused to the rooting processof the tissue culture seedlings.

The invention discloses a tissue culture medium for directly growing a seedling from a barley embryo. The tissue culture medium comprises a seedling growing culture medium and a root growing and seedling strengthening culture medium. A preparation method of the seedling growing culture medium comprises the following steps: firstly, regulating the pH value of every 1L of mother solution of the seedling growing culture medium to be 5.7-5.9; secondly, adding plant gel; thirdly, performing sterilization; and finally, adding filtered and sterilized vitamin B1, vitamin B6, nicotinic acid, 6-benzylaminopurine and 2,4-dichlorphenoxyacetic acid. A preparation method of the root growing and seedling strengthening culture medium comprises the following steps: firstly, adding inositol, casein hydrolysate, proline and malt sugar into every 1L of MS basic culture solution; secondly, regulating the pH value to be 5.8-6.0, then adding the plant gel and then performing the sterilization; and finally, adding the filtered and sterilized vitamin B1. The invention also provides a tissue culture method for directly growing the seedling from the barley embryo, and in the method, tissue culture medium is used.

The invention discloses a rapid propagation technology of rhizoma bletillae tissue culture seedlings. The rapid propagation technology includes the steps of 1), seed selection; 2), harvest of pods; 3), plant tissue culture including: A, disinfection; B, primary culture; C, subculture multiplication; D, root induction; E, acclimatization and transplant. With the technology, good provenance is guaranteed through seed selection, tissue culture time and cycle are greatly reduced through scientific tissue culture, survival rate of the tissue culture seedlings is more than 99%, and tubers obtained from culture of the tissue culture seedlings are detected to meet the standard according to Chinese pharmacopoeia.

The invention discloses a spider incense tissue culture medium and a culture method, wherein the tissue culture medium has the advantages of simple components, low cost and easy acquisition, can significantly improve the induction rate, proliferation coefficient, and rooting rate of spider incense, and can Effectively improve tissue culture efficiency, reduce production costs, and have market application value.

The invention provides a polygonatum cyrtonema immature embryo culture micro-stem tuber breeding method. The method comprises the following steps: selection of excellent single plant of polygonatum cyrtonema, stripping of immature seed immature embryos, induction culture of immature embryos, induction formation of micro stem tuber, propagation of micro stem tuber, and direct transplanting of micro stem tuber for seedling establishment. The polygonatum cyrtonema immature embryo culture has the advantages of rapid growth, strong differentiation force, and large propagation coefficient, and propagation and transplanting of the micro stem tuber have the advantages of fast propagation speed, large quantity, short period, convenient transplanting, storage resistance, transport resistance, direct transplanting, simple management and high survival rate; so that the seedling growing problems of slow breeding and seeding formation of the polygonatum cyrtonema seeds, low rhizome propagation coefficient and high cost in the prior art can be solved, polygonatum cyrtonema seedling can be rapidly breed, and the method is suitable for industrial large-scale production.

The invention discloses a rapid propagation method for seed tissue culture of Dendrobium Dendrobium drumsticks. The seeds of Dendrobium Dendrobium Dendrobium are sown in an induction medium to induce germination, and then the small buds of Dendrobium Dendrobium Dendrobium are transferred to a subculture medium and a rooting medium successively. Continue to cultivate in the middle to obtain Dendrobium Dendrobii rooting tissue culture seedlings, which can be transplanted into a greenhouse for hardening. The tissue culture method of the invention is simple, simple in steps and convenient in operation, and the obtained Dendrobium Dendrobii rooting tissue culture seedlings are robust and strong in growth ability, and are suitable for industrial production.

The invention discloses a rapid tissue culture method for lycium ruthenicum murr, belonging to the technical field of plant tissue culture. The rapid tissue culture method comprises the following steps: explants sterilization, induction culture, rooting culture, subculture, acclimatization and transplantation. According to the rapid tissue culture method, the rooting culture can be carried out during the subculture, so that the tissue culture is high, the operation is simple and convenient, the period is short, and complete plants can be rapidly acquired; the tissue culture process is not influenced by environmental factors.

The invention discloses a method for culturing and rapidly propagating tissue culture seedlings of lotus. The method comprises the following steps of (1) taking lotus germs on the 15th-18th day afterpollination as a material, inoculating the lotus germs into an aseptic seedling culture medium under an aseptic condition, and sealing and putting the aseptic seedling culture medium into a tissue culture room; (2) culturing for 3 months to obtain rooted stolon aseptic seedlings; (3) cutting each section of the stolon seedling into new buds, inoculating the new buds into a subculture medium for subculture, obtaining a large number of rooted stolon aseptic seedlings after one month, and repeating the steps until 5 times of subculture multiplication culture is carried out; and (4) transplantingthe stolon seedlings obtained in the step (3) to a greenhouse for acclimatization and domestication, and transplanting to a natural condition for growth after 3 weeks. According to the method, the embryo of the fresh lotus seed is taken as the explant in a breakthrough manner, the stolon seedling is successfully cultured, the success rate is up to 98%, the pollution rate is lower than 5%, the propagation coefficient of each subculture can reach 9.6, the period is short, the propagation efficiency is high, large-scale production of the lotus seedling is facilitated, and a new way is provided for meeting the market demand of the lotus seedling.

The invention discloses a method for establishing a rapid propagation system of tripterygium hypoglaucum. The method comprises the following steps: seeding terminal buds or axillary buds of tripterygium hypoglaucum into an induction culture medium to induce sprouting, subsequently transferring and transplanting small buds of tripterygium hypoglaucum into a subculture medium and a rooting medium in sequence to culture continuously, thereby obtaining root tissue culture seedlings of tripterygium hypoglaucum, and further transplanting the root tissue culture seedlings into a seedbed to harden the seedlings. The tissue culture method disclosed by the invention is simple and convenient, simple in step and convenient to operate, the prepared tripterygium hypoglaucum seedlings are healthy and strong, high in growing ability and applicable to large-scale production, the culture time is effectively shortened and the production cost is also lowered.