Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium for inducing sweet potato cluster buds

A culture medium and bud induction technology, which is applied in the field of culture medium for the induction of clustered buds of sweet potato, can solve the problems of time-consuming and labor-intensive efficiency, and achieve the effect of increasing the reproduction coefficient and improving the efficiency of tissue culture

Inactive Publication Date: 2014-06-11
QINGDAO BOITE BIOPHARM
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to overcome the problem of low reproductive coefficient in the shoot tip tissue culture of sweet potato, to provide a kind of medium for the efficient induction of clustered buds of sweet potato, which is used for the group of sweet potato Rapid Propagation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Method: Inoculate the stem apex meristems of sweet potato Xuzhou 18 on the culture medium, inoculate 1 stem apex meristem per test tube, place at 25 ℃, light intensity about 2000 Lux, daily light for 12 hours, and statistical results after 4 weeks of cultivation It can be seen that the induction rate of buds is 466%, the seedlings are slender and the growth rate is fast. Based on the above analysis, it is concluded that the best medium for the induction of sweet potato cluster buds is:

[0018] MS 1L

[0019] KT 1.0mg

[0020] 6-BA 1.0mg

[0021] IAA 0.2 mg

[0022] CH 200 mg

[0023] Sucrose 20 g

[0024] Agar 6g.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a culture medium for inducing sweet potato cluster buds, belonging to a sweet potato tissue culture technology. The culture medium is formed by dissolving kinetin (KT), 6-benzyl purine (6-BA), indoleacetic acid (IAA), casein hydrolysate (CH), cane sugar and agar into a basic culture medium (MS), wherein the dosage of all the components is as follows: 1L of MS, 1.0-2.0mg of KT, 1.0-2.0mg of 6-BA, 0.2-2.0mg of IAA, 200mg of CH, 20g of cane sugar and 6g of agar. The culture medium has the positive effects that after the culture medium is utilized, 8.6 buds can be induced by one stem apical meristem of a sweet potato, so that the induction efficiency is 860%, and the tissue culture efficiency is remarkably improved.

Description

technical field [0001] The invention relates to a medium for inducing clustered buds of sweet potato, which belongs to the technology of sweet potato tissue culture. In particular, a medium for inducing clustered shoots from the shoot apical meristem of sweet potato. Background technique [0002] The common method of culturing and propagating sweet potato shoot apical meristem is to induce differentiation of one shoot apical meristem into one bud, which is labor-intensive, time-consuming and inefficient. In the tissue culture and rapid propagation of many plants, the technology of inducing clustered buds is used to increase the reproduction coefficient and achieve twice the result with half the effort. Contents of the invention [0003] The purpose of the present invention is to overcome the problem of low reproduction coefficient in the tissue culture of sweet potato shoot tips, and provide a medium for efficiently inducing clustered buds of sweet potato, which is use...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 郝智慧符其中栾明娜
Owner QINGDAO BOITE BIOPHARM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com