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Scindapsus aureus cluster bud induced culture medium and scindapsus aureus cluster bud culture method

A culture method and bud induction technology, applied in the field of plant tissue culture, can solve problems affecting the research of radish, and achieve the effects of improving the reproduction coefficient and improving the efficiency of tissue culture

Inactive Publication Date: 2017-06-13
东方上彩现代农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] But at present, there is no medium suitable for the tissue culture of pothos, which seriously affects the research on the classic material of pothos

Method used

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  • Scindapsus aureus cluster bud induced culture medium and scindapsus aureus cluster bud culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A method for cultivating clustered buds of pothos, comprising the following steps:

[0064] (1) Prepare cluster bud induction medium: dissolve 6-benzylaminoadenine, indolebutyric acid, naphthaleneacetic acid, sucrose, banana puree and agar powder in MS basic medium, and cool to room temperature after autoclaving. The dosage of each component is 1L of MS basic medium, 0.05mg of 6-benzylaminoadenine, 1.0mg of indolebutyric acid, 0.5mg of naphthaleneacetic acid, 30g of sucrose, 30g of banana puree, and 7g of agar powder.

[0065] (2) Inoculation: Inoculate the callus of the pothos osmanthus into the cluster bud induction medium prepared in step (1) on the ultra-clean workbench and under aseptic conditions.

[0066] (3) Inducing and cultivating clustered buds: the inoculated pothos callus is sterile, the light intensity is 2000~30001x, the light time is 12h / d, the temperature is 25~28°C, and the humidity is 50~65% culture conditions Next, cultured for 30 days to obtain clu...

Embodiment 2

[0068] A method for cultivating clustered buds of pothos, comprising the following steps:

[0069] (1) Prepare cluster bud induction medium: dissolve 6-benzylaminoadenine, indolebutyric acid, naphthaleneacetic acid, sucrose, banana puree and agar powder in MS basic medium, and cool to room temperature after autoclaving. The dosage of each component is 1L of MS minimal medium, 0.08mg of 6-benzylaminoadenine, 1.5mg of indolebutyric acid, 0.5mg of naphthaleneacetic acid, 30g of sucrose, 30g of banana puree, and 7g of agar powder.

[0070] (2) Inoculation: Inoculate the callus of the pothos osmanthus into the cluster bud induction medium prepared in step (1) on the ultra-clean workbench and under aseptic conditions.

[0071] (3) Inducing and cultivating clustered buds: the inoculated pothos callus is sterile, the light intensity is 2000~30001x, the light time is 12h / d, the temperature is 25~28°C, and the humidity is 50~65% culture conditions Next, cultured for 30 days to obtain c...

Embodiment 3

[0073] A method for cultivating clustered buds of pothos, comprising the following steps:

[0074] (1) Prepare cluster bud induction medium: dissolve 6-benzylaminoadenine, indolebutyric acid, naphthaleneacetic acid, sucrose, banana puree and agar powder in MS basic medium, and cool to room temperature after autoclaving. The dosage of each component is 1L of MS minimal medium, 0.1mg of 6-benzylaminoadenine, 2.0mg of indolebutyric acid, 0.5mg of naphthaleneacetic acid, 30g of sucrose, 30g of banana puree, and 7g of agar powder.

[0075] (2) Inoculation: Inoculate the callus of the pothos osmanthus into the cluster bud induction medium prepared in step (1) on the ultra-clean workbench and under aseptic conditions.

[0076] (3) Inducing and cultivating clustered buds: the inoculated pothos callus is sterile, the light intensity is 2000~30001x, the light time is 12h / d, the temperature is 25~28°C, and the humidity is 50~65% culture conditions Next, cultured for 30 days to obtain cl...

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Abstract

The invention discloses a scindapsus aureus cluster bud induced culture medium. The scindapsus aureus cluster bud induced culture medium is prepared from an MS basic culture medium, 6-benzylamino adenine, indolebutyric acid, naphthylacetic acid, sucrose, mashed banana and agar powder. According to the scindapsus aureus cluster bud induced culture medium and a scindapsus aureus cluster bud culture method, disclosed by the invention, scindapsus aureus cluster buds are induced by utilizing calluses of scindapsus aureus to obtain a lot of homogeneous clones; the MS culture medium is a basic culture medium, and the 6-benzylamino adenine, the indolebutyric acid, the naphthylacetic acid plant growth regulator, which have specific concentrations, and the mashed banana, the sucrose and the agar powder are combined, so that the cluster bud induced culture medium can be effectively induced. The cluster bud induced culture medium is used for carrying out cluster bud culture on the calluses of the scindapsus aureus, and the induction efficiency can reach 92 percent or more; the reproduction coefficient of scindapsus aureus cluster buds is improved and the tissue culture efficiency is remarkably improved; scindapsus aureus tissue culture seedlings are easily industrially produced.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a medium for inducing bushy buds of pothos and a method for cultivating bushy buds of pothos. Background technique [0002] Green dill (scientific name: Epipremnum aureum), belonging to the Araceae genus Kylin, a large evergreen vine, likes a warm and humid environment, grows in tropical areas, often climbs and grows on rocks and tree trunks in the rainforest, and has strong entanglement and strong air. The roots are well developed and can be grown hydroponically. [0003] Pollen is one of the very good indoor decorative plants, climbing vines and foliage flowers. The radish stem is thin and soft, and the leaves are delicate and delicate. Set pots high on the top of the furniture cabinet, let the vines hang down calmly, or hang them in a circle after the vines hang too long, like emerald emerald reliefs. This not only makes full use of the space, purifies the air,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 王健刘坤申亮郑德助卢迪浩包宇飞李健施金玉
Owner 东方上彩现代农业有限公司
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