33 results about "Retardation factor" patented technology

Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.

A liquid chromatograph control apparatus (1) includes a measured value storage (25a) that stores a measured retardation factor (Rf) value of a sample (3), which is obtained when components of the sample are separated on thin layer chromatography using eluent (4) containing a plurality of ingredients at a specified mixture ratio, in association with the specified mixture ratio, a rate-of-change-in-Rf-value storage (25b) that stores a rate of change in the Rf value of the sample (3) with respect to variation in mixture ratio of ingredients of eluent (10), a mixture ratio calculator (22a) that determines a mixture ratio of the eluent (10) at which a specified retardation factor value (RfO) of the sample is obtained, based on the measured Rf value corresponding to the specified mixture ratio stored in the measured value storage (25a) and on the rate of change in the Rf value stored in the rate-of-change-in-Rf-value storage (25b), and a mixture ratio controller (22b) that outputs a control signal to control the mixture ratio of the eluent (4) fed into a column (18) so that the Rf value of the sample (3) can be equivalent to the specified retardation factor value (RfO), based on calculated results by the mixture ratio calculator (22a).

The invention provides a narrow-band spiral chromatographic sheet and a preparation method of the narrow-band spiral chromatographic sheet. The narrow-band spiral chromatographic sheet comprises a substrate, wherein a spiral-line-shaped groove is formed at one side of the substrate, the starting end of the groove is positioned at the position of the geometric center of the substrate, and a stationary phase is arranged in the groove; according to the narrow-band spiral chromatographic sheet, the open range is increased, the difference of the retardation factors of the mixtures is increased under the action of the centrifugal force when the mixture is separated, and the separating efficiency is improved.

The invention relates to a rapid detection method of chemical components illegally added in Chinese patent medicine Xiaohuoluo pills. The method comprises the following steps: firstly, preparing a reference solution and a sample solution; respectively suctioning the reference solution and the sample solution; dripping on the same silica gel thin layer plate; adding a developing agent for ascending development; taking out and drying in the air; placing below an ultraviolet lamp and observing thin-layer chromatogram; spraying sulfuric acid-ethanol solution; placing in an oven and heating at a constant temperature until spots on the thin layer plate are clear; respectively placing below a fluorescent lamp and the ultraviolet lamp and observing thin-layer chromatogram; and determining that the sample result is positive if fluorescence quenching spots, fluorescence spots or spots with the same color occur at the same time at a point with a retardation factor value same as that of the reference. Obviously, the method provided by the invention is simple to operate, rapid, and cost-effective, not only solves the problem of false positive interference, but also avoids expensive cost and plenty of time consumed by the conventional infrared spectroscopy, HPLC-MS and the like, thereby making it possible to effectively monitor whether chemical components are illegally added in the Xiaohuoluo pills.

The invention provides an aeration zone soil adsorption parameter tester based on strong adsorption pollutants. The aeration zone soil adsorption parameter tester comprises a detachable soil column, a buffering section, a soil column fixing device, a sampling device, a constant water head fixing device, a constant water head water feeding device, a constant water head liquid supplying device and a water supplying and liquid supplying switching device, wherein a soil sample is put into the detachable soil column; the detachable soil column is composed of a soil column inner ring, a soil column outer ring, an inner ring handle and an outer ring handle; the buffering section is composed of an organic glass tube, a gauze element A, a gauze element B, an organic glass plate with holes and a quartz sand filtering layer; the constant water head water feeding device and the constant water head liquid supplying device are mounted on the constant water head fixing device, and the upper and lower positions of the constant water head water feeding device and the constant water head liquid supplying device can be adjusted; the bottom of the constant water head water supplying device is connected with a water outlet pipe; and the bottom of the constant water head liquid supplying device is connected with a liquid outlet pipe. The device can be used for accurately determining retardation factors and distribution factors of the pollutants with the relatively strong adsorption effect in aeration zone soil; and meanwhile, the method is simple and reliable.

The invention provides an aeration zone soil adsorption parameter tester based on weak adsorption pollutants. The aeration zone soil adsorption parameter tester at least comprises a sealing cover, an experiment section, a sampling device, a constant water head fixing device, a constant water head water feeding device, a constant water head liquid supplying device and a water supplying and liquid supplying switching device, wherein the experiment section is composed of a soil column, gauze elements, an organic glass plate with holes, a quartz sand filtering layer and a soil column bracket; the sealing cover is mounted at the top of the soil column and is used for sealing the top of the soil column; the sampling device is arranged on the upper part of the soil column; the constant water head water feeding device and the constant water head liquid supplying device are mounted on the constant water head fixing device, and the upper and lower positions of the constant water head water feeding device and the constant water head liquid supplying device can be adjusted; the bottom of the constant water head water supplying device is connected with a water outlet pipe; the bottom of the constant water head liquid supplying device is connected with a liquid outlet pipe; and the water outlet pipe, the liquid outlet pipe and an input pipe are connected with the water supplying and liquid supplying switching device. The device can be used for accurately determining retardation factors and distribution factors of the pollutants with the relatively weak adsorption effect in aeration zone soil; and meanwhile, the method is simple and reliable.

The invention relates to a method for rapidly screening 10 illegally added chemicals in small collateral activating pills. The method comprises the following steps: firstly, preparing a reference sample solution and a test solution; then respectively sucking the reference sample solution and the test solution, dripping the sucked reference sample solution and the test solution on the same silica gel thin layer plate, carrying out ascending development by a developing solvent, taking out the silica gel thin layer plate and air-drying; placing the silica gel thin layer plate under an ultraviolet lamp to observe thin-layer chromatographies; spraying an ethanol sulfate solution; placing the thin layer plate into an oven to carry out constant-temperature heating until colors of spots on the thin layer plate are clearly developed; then respectively placing the thin layer plate under sunlight and the ultraviolet lamp to observe thin layer chromatographies; if fluorescence quenching spots, fluorescence spots or spots with the same color are simultaneously generated at the position with the same retardation factor value with a reference sample, judging that the sample result is positive. Obviously, the method is simple, convenient and rapid to operate, is low in cost, not only solves the problem of false positive interference, but also avoids high cost and long time, which are generated when an infrared spectroscopy, HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry) and the like are adopted to carry out measurement conventionally, and enables effective monitoring on the illegally added chemicals in the Chinese patent medicine small collateral activating pills to be possible.