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Method for fast separating polysaccharides from dragon fruit by counter-current chromatography

A technology of countercurrent chromatography and dragon fruit is applied in the fields of food processing and utilization and functional food manufacturing, which can solve the problems of slow speed, limited amount of separation and high cost, and achieve the expansion of application range, fast separation speed and large injection volume. Effect

Inactive Publication Date: 2017-06-13
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing methods for extracting and preparing polysaccharides mainly use methods such as gel column chromatography, membrane separation, and ion exchange chromatography. The limitations are that the materials (fillers or membranes) used are expensive, costly, and slow. The amount of each separation is limited
Therefore, the existing polysaccharide separation technology is difficult to meet the needs of the separation of dragon fruit polysaccharides
[0004] At present, there have been reports on the separation of laminarin, lentinan, and spirulina polysaccharides by countercurrent chromatography, but the separation conditions and methods reported by countercurrent chromatography are not suitable for the separation of dragon fruit polysaccharides

Method used

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  • Method for fast separating polysaccharides from dragon fruit by counter-current chromatography
  • Method for fast separating polysaccharides from dragon fruit by counter-current chromatography
  • Method for fast separating polysaccharides from dragon fruit by counter-current chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, a kind of method (utilize counter-current chromatography to separate the method for polysaccharide rapidly from pitaya) of polysaccharide from pitaya, carry out following steps successively:

[0047] (1) Peel the dragon fruit and take the pulp, slice the pulp to a thickness of 3-5mm, and freeze-dry it at a temperature of -50-55°C and a vacuum of 50-100Pa until it is made into a product with a water content of ≤0.05%. Dried dragon fruit slices (drying time is about 24h).

[0048] Put 200g of dried dragon fruit slices into a blender and crush until passing through a 200-300 mesh sieve to obtain dried dragon fruit powder, add it to a large beaker filled with 8.0L distilled water, heat to boiling, keep at 100°C, cook and extract for 3 hours ;

[0049] Replace the dried pitaya slices with the residue obtained after the extraction and repeat the above-mentioned boiling water extraction for 2 times, and combine all the extracts.

[0050] (2) The combined extracts...

Embodiment 2

[0062] Embodiment 2, a kind of method for separating and preparing polysaccharide from pitaya, carries out following steps successively:

[0063] (1), the preparation method of dragon fruit dried slice is the same as embodiment 1.

[0064] Put 180g of dried dragon fruit slices into a blender and crush until passing through a 200-300 mesh sieve to obtain dried dragon fruit powder, add it to a large beaker filled with 7.0L distilled water, heat to boiling, keep at 100°C, cook and extract for 2.5 h;

[0065] Replace the dried pitaya slices with the residue obtained after the extraction and repeat the above-mentioned boiling water extraction for 2 times, and combine all the extracts.

[0066] (2) The combined extracts are cooled to room temperature and filtered, and the filtrate is concentrated, depigmented, deproteinized in turn, and concentrated again to obtain a concentrated solution;

[0067] details as follows:

[0068] Concentrate the filtrate (concentrate with a rotary e...

Embodiment 3

[0073] Embodiment 3, a kind of method for separating and preparing polysaccharide from pitaya, carries out following steps successively:

[0074] (1), the preparation method of dragon fruit dried slice is the same as embodiment 1.

[0075] Put 180g of dried dragon fruit slices into a grinder and grind until passing through a 200-300 mesh sieve to obtain dried dragon fruit powder, add it to a large beaker filled with 8.0L distilled water, heat to boiling, keep at 100°C, cook and extract for 3 hours ;

[0076] Replace the dried pitaya slices with the residue obtained after the extraction and repeat the above-mentioned boiling water extraction for 2 times, and combine all the extracts.

[0077] (2) The combined extracts are cooled to room temperature and filtered, and the filtrate is concentrated, depigmented, deproteinized in turn, and concentrated again to obtain a concentrated solution;

[0078] details as follows:

[0079] Concentrate the filtrate (concentrate with a rotar...

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Abstract

The invention discloses a method for fast separating polysaccharides from dragon fruit by counter-current chromatography. The method comprises the following steps of crushing dry dragon fruit slices; then, performing leaching extraction by boiling water; cooling leaching extraction liquid to room temperature; then, filtering; sequentially performing concentration, depigmentation and deproteinization on the filter liquid; performing concentration again to obtain concentration liquid; adding absolute ethyl alcohol into the concentration liquid for precipitation; performing freeze drying on the obtained precipitates to obtain dragon fruit coarse polysaccharides; taking 0.25 to 0.35g of dragon fruit coarse polysaccharides to be dissolved into 25+ / -5ml of a lower phase of a double-water-phase solvent system; injecting into the counter-current chromatography; performing pH regulation on the upper phase of the double-water-phase solvent system so as to respectively obtain a first flowing phase, a second flowing phase and a third flowing phase; respectively taking the first flowing phase, the second flowing phase and the third flowing phase for elution; collecting elution liquid with the same retardation factor value in each flowing phase; and respectively performing concentration, dialysis and freeze drying on the three kinds of collected elution liquid so as to respectively obtain three kinds of dragon fruit polysaccharides.

Description

technical field [0001] The invention belongs to the field of food processing and utilization and functional food manufacturing. It mainly uses chromatographic methods and chemical methods to extract and separate biologically active substances or functional components from biological materials that have utility value or contain biologically active components. In order to study the activity of this component Mechanism or development of new products, etc. to provide technical support. Background technique [0002] Dragon fruit (Hylocereus undulates Britt), known as "fairy fruit" and "auspicious fruit", is a nutritious fruit suitable for all ages. It contains polysaccharides, organic acids, dietary fiber and potassium, calcium, magnesium, phosphorus and other Nutrients. According to reports, dragon fruit polysaccharides have good functions in promoting intestinal peristalsis and preventing constipation. [0003] The extraction and separation of functional polysaccharides is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 宋广磊于达
Owner ZHEJIANG GONGSHANG UNIVERSITY
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