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Multi-enzyme-modified microcrystalline Chinese yam powder for relaxing bowel and preparation method thereof

A technology of laxative and laxative yam powder, which is applied in the field of preparation of multi-enzyme modified microcrystalline yam powder, can solve the problems of unfavorable consumption by diabetic patients in large quantities and weak effect of laxative laxative, and achieve laxative laxative effect, Improve intestinal propulsion and relieve constipation

Inactive Publication Date: 2016-02-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the pharmacological substances in yam are small molecular components. However, yam also contains a lot of starch, which will be converted into glucose in a large amount during human metabolism, which is not conducive to diabetics to eat in large quantities. At the same time, the effect of laxative and laxative is weak

Method used

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  • Multi-enzyme-modified microcrystalline Chinese yam powder for relaxing bowel and preparation method thereof
  • Multi-enzyme-modified microcrystalline Chinese yam powder for relaxing bowel and preparation method thereof
  • Multi-enzyme-modified microcrystalline Chinese yam powder for relaxing bowel and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of a multi-enzyme modified microcrystalline yam powder with laxative effect, comprising the steps:

[0022] (1) Weigh the whole yam powder passed through a 200-mesh sieve, add 5 times the mass of acetic acid-sodium acetate buffer solution with pH=5.2, and mix well; stir at 80°C for 30 minutes to obtain a suspension;

[0023] (2) Add α-amylase to the suspension so that the ratio of α-amylase to whole yam powder is 6U:1g, stir at 83°C for 40min; cool to 45°C, add pullulanase to make the pullulan The ratio of blue enzyme to whole yam powder is 2-6U / g, stir at 40-50°C for 20-28h; cool to 4°C and place for 24h; heat in boiling water bath for 90min to inactivate the enzyme, and obtain the enzymolysis solution;

[0024] (3) Wash the enzymolyzate with 80% ethanol aqueous solution until the supernatant is clear, filter, dry the precipitate, pulverize and sieve. The recovery rate of the multi-enzyme modified microcrystalline yam powder with laxative effect c...

Embodiment 2

[0026] A preparation method of a multi-enzyme modified microcrystalline yam powder with laxative effect, comprising the steps:

[0027] (1) Weigh the whole yam powder passed through a 200-mesh sieve, add 4 times the mass of acetic acid-sodium acetate buffer solution with pH=5.2, and mix well; stir at 75°C for 40 minutes to obtain a suspension;

[0028] (2) Add α-amylase to the suspension so that the ratio of α-amylase to whole yam powder is 4U: 1g, stir at 85°C for 30min; cool to 40°C, add pullulanase to make the pullulan The ratio of blue enzyme to whole yam powder is 2-6U / g, stirred at 40-50°C for 20-28h; cooled to 4°C and left for 28h; heated in a boiling water bath for 90min to inactivate the enzyme to obtain the enzymolysis solution;

[0029] (3) Wash the enzymolyzate with 80% ethanol aqueous solution until the supernatant is clear, filter, dry the precipitate, pulverize and sieve. The recovery rate of the multi-enzyme modified microcrystalline yam powder with laxative e...

Embodiment 3

[0031] A preparation method of a multi-enzyme modified microcrystalline yam powder with laxative effect, comprising the steps:

[0032] (1) Weigh the whole yam powder passed through a 200-mesh sieve, add 6 times the mass of acetic acid-sodium acetate buffer solution with pH=5.2, and mix well; stir at 85°C for 20 minutes to obtain a suspension;

[0033] (2) Add α-amylase to the suspension so that the ratio of α-amylase to whole yam powder is 8U:1g, stir at 80°C for 50min; cool to 50°C, add pullulanase to make the pullulan The ratio of blue enzyme to whole yam powder is 2-6U / g, stir at 40-50°C for 20-28h; cool to 4°C for 20h; heat in a boiling water bath for 90min to inactivate the enzyme, and obtain the enzymolysis solution;

[0034] (3) Wash the enzymolyzate with 80% ethanol aqueous solution until the supernatant is clear, filter, dry the precipitate, pulverize and sieve. The recovery rate of the multi-enzyme modified microcrystalline yam powder with laxative effect can reach...

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Abstract

The present invention discloses multi-enzyme-modified microcrystalline Chinese yam powder for relaxing bowel and a preparation method thereof. The preparation method includes the following steps: (1) weighting whole Chinese yam powder, adding acetic acid-sodium acetate buffer (with pH being 5.2) into the whole Chinese yam powder and evenly mixing the mixture; and stirring the mixture to obtain suspension; (2) adding alpha-amylase into the suspension and stirring the suspension; adding pullulanase into the suspension and stirring the suspension; cooling the suspension to 4 DEGC and standing; and heating the suspension in boiling water bath to inactivate enzymes to obtain enzymatic hydrolysate; and (3) washing the enzymatic hydrolysate with aqueous ethanol solution until the supernatant is clear and transparent, filtering the enzymatic hydrolysate, and drying, crushing and sieving precipitates. Through the preparation method, the Chinese yam powder is modified by two biological enzymes, and the Chinese yam powder has significant changes in the aspects of micro-morphology and physical and chemical properties of. The multi-enzyme-modified microcrystalline Chinese yam powder can prevent and relieve constipation, and pharmacological experiments prove that the multi-enzyme-modified microcrystalline Chinese yam powder can improve the intestinal propulsion, and has a significant effect in relaxing bowel.

Description

technical field [0001] The invention belongs to the field of health food, in particular to a preparation method of multi-enzyme modified microcrystalline yam powder with laxative effect. Background technique [0002] In modern society, with the great abundance of material life, the incidence of constipation is on the rise rapidly, and most of them are functional constipation, mostly due to eating too finely, drinking too little water, lack of exercise, bad bowel habits and intestinal irritability Syndrome, etc. As a result, in recent years, products (mostly health food) that can moisten the intestines and laxatives have sprung up like mushrooms. Most of the traditional Chinese medicine products that can relieve bowel movements contain senna leaf and rhubarb. Although senna leaf and rhubarb are two traditional Chinese medicines often used in traditional Chinese medicine, doctors will not only strictly control the dosage, but also give medicines according to the actual situat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/214A23L1/29A61K36/8945A61K9/14A61P1/10
Inventor 高文远李霞王婷婷黄含含
Owner TIANJIN UNIV
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