Method for rapid screening of 10 kinds of illegally added chemicals in Xiaohuoluo Pills

Abstract

The invention relates to a method for rapidly screening 10 illegally added chemicals in small collateral activating pills. The method comprises the following steps: firstly, preparing a reference sample solution and a test solution; then respectively sucking the reference sample solution and the test solution, dripping the sucked reference sample solution and the test solution on the same silica gel thin layer plate, carrying out ascending development by a developing solvent, taking out the silica gel thin layer plate and air-drying; placing the silica gel thin layer plate under an ultraviolet lamp to observe thin-layer chromatographies; spraying an ethanol sulfate solution; placing the thin layer plate into an oven to carry out constant-temperature heating until colors of spots on the thin layer plate are clearly developed; then respectively placing the thin layer plate under sunlight and the ultraviolet lamp to observe thin layer chromatographies; if fluorescence quenching spots, fluorescence spots or spots with the same color are simultaneously generated at the position with the same retardation factor value with a reference sample, judging that the sample result is positive. Obviously, the method is simple, convenient and rapid to operate, is low in cost, not only solves the problem of false positive interference, but also avoids high cost and long time, which are generated when an infrared spectroscopy, HPLC-MS (High Performance Liquid Chromatography-Mass Spectrometry) and the like are adopted to carry out measurement conventionally, and enables effective monitoring on the illegally added chemicals in the Chinese patent medicine small collateral activating pills to be possible.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicines, in particular to a method for quickly screening 10 kinds of illegally added chemicals in Xiaohuoluo pills. Background technique [0002] Xiaohuoluo Pills is a traditional Chinese medicine preparation composed of 6 Chinese medicine decoction pieces including Dannanxing, Zhichuanwu, Zhicaowu, Dilong, frankincense (made) and myrrh (made). The main effects are dispelling wind and cold, resolving phlegm and dehumidification, promoting blood circulation and relieving pain. It is used for arthralgia caused by wind-cold-damp pathogen blockage and phlegm-stasis blockage of collaterals. Symptoms include limb joint pain, or cold pain, or tingling pain, or pain that is worse at night, joint flexion and extension difficulties, numbness and spasm. Its preparation method is to grind Dannanxing, Zhichuanwu, Zhicaowu, Dilong, frankincense (made), and myrrh (made) into fine powder...

AI Technical Summary

Problems solved by technology

However, because infrared spectrometers, HPLC-MS, and GC-MS instruments are expensive and must be operated by professional technicians, the maintenance costs are also extremely high, and rapid screening cannot be achieved to achieve the purpose of monitoring

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