The invention relates to a dynamic taint analysis device and application of the dynamic taint analysis device to document format reverse analysis, wherein the dynamic taint analysis device comprises a dynamic instrumentation executive logging module and a static snapshoot analysis taint tracking module, wherein the dynamic instrumentation executive logging module is used for calling and executing a tested program by using a binary program instrumentation platform, monitoring opening, analyzing and closing behaviors of a data document including original taint data in a process of executing the tested program, and acquiring snapshoot logs of all command processes, context information and memory access information in the process of executing the tested program according to the process of executing the tested program; the static snapshoot analysis taint tracking module is used for analyzing the snapshoot logs and simulating replay execution of a progress according to information obtained by analysis and recording processing and spread information of original taint data in the data document to obtain a taint data stream path. According to the dynamic taint analysis device, I / O (Input / Output), time and space overhead of dynamic taint analysis in a dynamic execution process can be reduced, an extended instruction set can be supported, and the continuity and incidence relation of original taint data can be obtained.