Manufacturing method of 3D printing assemblable paper-based micro-fluidic chip

A microfluidic chip and 3D printing technology, which is applied in the fields of engineering and biosensing, can solve the problems that paper-based microfluidic chips cannot be designed and manufactured, microchannel construction is not precise enough, and the operation process is complicated, so as to achieve fast and convenient analysis The effect of detection, broad commercialization prospects, and simple operation

Pending Publication Date: 2022-02-18
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned methods are either expensive to manufacture, or the operation process is complicated, or the microchannel construction is not precise enough, and it is impossible to design and manufacture a paper-based microfluidic chip with a real complex structure.

Method used

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  • Manufacturing method of 3D printing assemblable paper-based micro-fluidic chip
  • Manufacturing method of 3D printing assemblable paper-based micro-fluidic chip
  • Manufacturing method of 3D printing assemblable paper-based micro-fluidic chip

Examples

Experimental program
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Effect test

Embodiment 1

[0032] In order to avoid the interference of the external environment and build a closed channel, the paper-based functional module should be in close contact with the motherboard during assembly. In the same way, each paper-based functional module should be closely attached to ensure that the fluid can be transported quickly. According to this principle, paper-based microfluidic chips with various classical channel styles were assembled to demonstrate that they can construct various detection and analysis modes.

[0033] like figure 2 As shown, the motherboard 1 and the paper-based functional module 2 can be flexibly assembled and spliced ​​into mixed channels of different modes, such as straight-line channels, criss-cross channels, Y-shaped channels and T-shaped channels.

Embodiment 2

[0035] A 3D-printable assembleable paper-based microfluidic chip and its manufacturing method draw the linear relationship for instant detection of hydrogen peroxide as follows:

[0036] like image 3 As shown in the figure, the device was built, the detection area 9 was immersed in 5U HRP solution for pretreatment, and incubated in a constant temperature incubator at 37 °C for 10 min. 0 μmol / L, 100 μmol / L, 500 μmol / L, 1 mmol / L, 5 mmol / L and 10 mmol / L of hydrogen peroxide were added dropwise to sample port 8 respectively, and the two were mixed in the “T” channel. Flow to the detection area 9, react with HRP for 8 minutes and turn blue, remove the detection area 9 module and dry it with nitrogen, place it on the groove 3, and move the groove 3 to the bottom card slot of the detection part. The smartphone is assembled above the signal collection area 5, and the entire device is wrapped with tin foil to eliminate interference from external light sources. Turn on the flash of t...

Embodiment 3

[0039] A 3D-printable assembleable paper-based microfluidic chip and its manufacturing method. The detection steps of hydrogen peroxide in a commercially available 3% hydrogen peroxide disinfectant are as follows:

[0040] According to the method described in Example 2, after diluting the commercially available 3% hydrogen peroxide disinfectant 1000 times, quantitative detection was carried out on the T-shaped paper chip, and compared with the classic potassium permanganate titration method.

[0041] As shown in table 1, the difference of two kinds of detection methods is less, and content is all at 2.50%-3.50%, all can prove that the content of this hydrogen peroxide disinfectant meets Chinese Pharmacopoeia standard. As shown in Table 2, in the standard addition recovery experiment, the standard addition recovery rate of this device is between 93.26%-103.06%, indicating that the present invention detects the high accuracy of hydrogen peroxide in real time.

[0042] Table 1 is...

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Abstract

The invention discloses a manufacturing method of a 3D printing assemblable paper-based micro-fluidic chip. Based on the characteristics of low cost, portability and easy integration of a paper-based micro-fluidic chip, the paper-based micro-fluidic chip is combined with a smart phone mobile terminal, a new technology capable of quantitatively and instantly detecting hydrogen peroxide is established, and instant detection of a medical hydrogen peroxide disinfectant is realized. The smart phone mobile is used for photographing, Image J software is used for data analysis, simple and convenient instant hydrogen peroxide detection is achieved, and a new technology is provided for manufacturing a multifunctional portable instant detection platform.

Description

technical field [0001] The invention belongs to the technical field of engineering and biosensing, and more specifically relates to a 3D printing assembling paper-based microfluidic chip and a manufacturing method thereof. Background technique [0002] Hydrogen peroxide, also known as hydrogen peroxide, is a strong oxidizing agent that is easily soluble in water and is widely used in medical, military, and chemical fields. At the same time, active oxygen, mainly hydrogen peroxide, is an important signaling molecule in the human body, which is of great significance to maintaining the redox balance of cells in the body. Therefore, it is of great practical significance to be able to quickly and conveniently detect hydrogen peroxide. [0003] Paper-based microfluidic chip is a rapid detection analysis platform, which has the advantages of low price, simple operation, good biocompatibility, and adaptability to various equipment. At the same time, the capillary action of the pap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B29C64/118B29C64/124B29C64/379B29C64/393B33Y10/00B33Y40/20B33Y80/00B01L3/00
CPCB29C64/118B29C64/124B29C64/379B29C64/393B33Y10/00B33Y40/20B33Y80/00B01L3/5027B01L2200/027
Inventor 刘爱林刘萌萌杨元杰刘辉雷云
Owner FUJIAN MEDICAL UNIV
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