Preparation method of fish skin collagen peptide jelly capable of being stored and transported at normal temperature
A fish skin collagen and collagen peptide technology, which is applied in the field of food engineering and functional food, can solve the problems of unfavorable sustainable development and limited economic benefits, and achieve the effects of increasing added value, widening the audience, and enriching the taste
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0038] This embodiment provides a method for making fish skin collagen peptide jelly that can be stored and transported at room temperature, including the following steps:
[0039] (1) Extraction of collagen: Homogenize the fish skin, add purified water 10 times the weight of the fish skin to the homogenized fish skin, stir and extract in a water bath at 90°C for 2 hours, filter After filtration, centrifuge at 5000r / min for 15min, and the supernatant is the crude collagen extract.
[0040] (2) Extraction of collagen peptides: the crude collagen extract obtained in the step (1) is added at 35-55°C with a compound protease combined with various enzyme systems, and the enzymatic hydrolysis time is 1.5h, and the enzyme preparation is added The amount is 0.3% of the total weight of fish skin, and then the temperature is raised to 121° C. for 10 minutes to inactivate the complex protease, and finally a crude collagen peptide extract is obtained.
[0041] The compound protease used ...
Embodiment 2
[0052] The mensuration of embodiment 2 hydroxyproline content
[0053] Amino acid determination was carried out according to the fish skin collagen peptide jelly prepared in Example 1.
[0054] 1. Instrument
[0055] Electric constant temperature blast drying oven, BP221S (Sartorius) electronic balance, 721 spectrophotometer, HWS26 electronic constant temperature water bath, etc.
[0056] 2. Reagents and preparation
[0057] (1) 6mol / L hydrochloric acid, 0.01mol / L hydrochloric acid, 0.1mol / L NaOH solution.
[0058] (2) Citric acid buffer solution: Dissolve 120g of sodium citrate, 12mL of glacial acetic acid, 46g of citric acid, and 34g of sodium hydroxide in distilled water, and adjust the volume to 1000mL, and adjust the pH to 6.0 before use.
[0059] (3) 0.05mol / L chloramine T solution: Weigh 7.05g of chloramine T, dissolve it in 100mL of distilled water, then add 150mL of ethylene glycol monomethyl ether, then add 250mL of citric acid buffer and mix well.
[0060] (4) p...
Embodiment 3
[0082] Example 3 Collagen peptide extraction rate and gel strength measurement
[0083] Instruments and Equipment
[0084] Electric constant temperature water bath Shanghai Yiheng Scientific Instrument Co., Ltd.; Texture Analyzer FTC Company of the United States; FD-3 Freeze Dryer Beijing Boyikang Experimental Instrument Co., Ltd.
[0085] Determination of Collagen Peptide Extraction Rate
[0086] Collagen peptide is hydrolyzed by a hydrochloric acid solution containing a certain amount of stannous chloride to release hydroxyproline, and after being oxidized by the oxidant chloramine T, it can react with p-dimethylaminobenzaldehyde solution at a certain temperature to form a red complex , has a maximum absorption peak at a wavelength of 550nm, therefore, it can be quantitatively determined by UV spectrophotometry. Hydroxyproline content was determined using a Hydroxyproline (Hyp) test kit (digestion method). Collagen peptide content and collagen peptide yield were calculate...
PUM
Property | Measurement | Unit |
---|---|---|
strength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com