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Dual-network bio-ink as well as preparation method and application thereof

A bio-ink, dual-network technology, applied in the field of 3D printing and biomedical materials, can solve the problems of printing, cell damage, unfavorable large structures for a long time, etc. Effect

Pending Publication Date: 2021-11-02
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, UV curing is commonly used at present. During the preparation process, long-term UV exposure may cause potential cell damage, which is not conducive to the long-term printing process of large structures.
Although it has been reported in the literature that visible light is used, it uses strong near-ultraviolet blue light of 405nm, and long-term exposure can also cause cell damage

Method used

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  • Dual-network bio-ink as well as preparation method and application thereof
  • Dual-network bio-ink as well as preparation method and application thereof
  • Dual-network bio-ink as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of double network bio-ink (3% methacrylated hyaluronic acid / 0.5% intervertebral disc cartilage decellularized matrix, abbreviated as 3% MeHA / 0.5% dECM)

[0052] A double-network bio-ink for 3D printing, the preparation raw materials are composed of methacrylated hyaluronic acid, digested intervertebral disc cartilage decellularized matrix powder, eosin Y, triethanolamine, N-vinylpyrrolidone and solvent, and its preparation method as follows:

[0053] 1) 1g of hyaluronic acid and 3ml of methacrylic anhydride in an aqueous solution, in an ice-bath alkaline environment, after the reaction, dialyzed and freeze-dried to prepare methacrylated hyaluronic acid;

[0054] 2) Preparation of digested intervertebral disc cartilage decellularized matrix powder: after rinsing the fresh intervertebral disc cartilage taken out from oxtail with deionized water, use 0.25% trypsin in EDTA solution containing 100 U / ml DNase and 10 U / ml RNA Enzyme mixed Tris-HCl soluti...

Embodiment 2

[0057] Example 2 Preparation of double network bio-ink (3% methacrylated hyaluronic acid / 1% decellularized matrix of intervertebral disc cartilage, abbreviated as 3% MeHA / 1% dECM)

[0058] A double-network bio-ink for 3D printing, the preparation raw materials are composed of methacrylated hyaluronic acid, digested intervertebral disc cartilage decellularized matrix powder, eosin Y, triethanolamine, N-vinylpyrrolidone and solvent, and its preparation method as follows:

[0059] 1) 1g of hyaluronic acid and 3ml of methacrylic anhydride in an aqueous solution, in an ice-bath alkaline environment, prepared by dialysis and freeze-drying after reaction;

[0060] 2) Preparation of digested intervertebral disc cartilage decellularized matrix powder: after rinsing the fresh intervertebral disc cartilage taken out from oxtail with deionized water, use 0.25% trypsin in EDTA solution containing 100 U / ml DNase and 10 U / ml RNA Enzyme mixed Tris-HCl solution (pH=7.4), penicillin and strept...

Embodiment 3

[0063] The preparation of embodiment 3 double network bioink

[0064] A double-network bio-ink for 3D printing, the preparation raw materials are composed of methacrylated hyaluronic acid, digested intervertebral disc cartilage decellularized matrix powder, eosin Y, triethanolamine, N-vinylpyrrolidone and solvent, and its preparation method as follows:

[0065] 1) 1g of hyaluronic acid and 3ml of methacrylic anhydride in an aqueous solution, in an ice-bath alkaline environment, prepared by dialysis and freeze-drying after reaction;

[0066] 2) Preparation of digested intervertebral disc cartilage decellularized matrix powder: after rinsing the fresh intervertebral disc cartilage taken out from oxtail with deionized water, use 0.25% trypsin in EDTA solution containing 100 U / ml DNase and 10 U / ml RNA Enzyme mixed Tris-HCl solution (pH=7.4), penicillin and streptomycin double-antibody aqueous solution with a volume fraction of 3% were repeatedly treated to obtain the acellular ma...

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Abstract

The invention discloses dual-network bio-ink as well as a preparation method and application thereof. The dual-network bio-ink is prepared from the following raw materials: a free radical photocurable natural polymer material, an acellular matrix, a visible light initiator, a co-initiator and a solvent, and the visible light initiator is an initiator suitable for visible light with the wavelength of 450-550 nm. When the acellular matrix is digested acellular matrix powder, the preparation method comprises the following steps: 1) dissolving the digested acellular matrix powder and a free radical photocurable natural polymer material in an acid solution, under an ice bath condition, adjusting the pH value to 7-8, and adding a PBS solution or a culture medium; and 2) adding an initiator and a co-initiator into the mixture obtained in the step 1), and uniformly mixing to obtain the dual-network bio-ink. The bio-ink disclosed by the invention has good biological activity and visible light 3D printing property.

Description

technical field [0001] The invention belongs to the technical fields of 3D printing and biomedical materials, and in particular relates to a novel double-network bio-ink that can be used for 3D bio-printing, its preparation method and application. Background technique [0002] 3D bioprinting is a technology that rapidly manufactures bioactive scaffold materials of a specified shape through the accurate 3D accumulation of materials, cells, and growth factors through the transformation of the model after the computer-aided design model of the printed object or the data obtained by computerized tomography. At present, although the in vivo implants manufactured by 3D printing methods can have certain biological activities, there are still great challenges in manufacturing organs or tissues that are truly suitable for in vivo implantation, and the most critical part is Development of suitable bioinks, biomaterials that can support cell survival and maintain factor activity. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/50B33Y10/00B33Y80/00B33Y70/10
CPCA61L27/3633A61L27/20A61L27/50B33Y10/00B33Y80/00B33Y70/10C08L5/08
Inventor 赖毓霄郑子卓秦岭
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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