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Aleuritopteris argentea polysaccharide and preparation method and application thereof

A technology for clearing herbs and polysaccharides, applied in the field of medicine, can solve the problem of unclear polysaccharide composition, and achieve the effects of significant antioxidant activity, improved extraction rate, and significant anti-inflammatory effect.

Active Publication Date: 2021-10-22
HUAQIAO UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above-mentioned prior art studies on the polysaccharides of Tongjing grass are limited to the level of crude polysaccharides, and the polysaccharide components are not clear. Therefore, it is necessary to carry out in-depth research on the preparation method and characteristics of Tongjing grass polysaccharides, in order to be a substitute for natural non-toxic and side effects drug components. Product development provides new objects

Method used

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  • Aleuritopteris argentea polysaccharide and preparation method and application thereof
  • Aleuritopteris argentea polysaccharide and preparation method and application thereof
  • Aleuritopteris argentea polysaccharide and preparation method and application thereof

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preparation example Construction

[0038] A method for preparing polysaccharides of Tongjing grass, comprising the steps of:

[0039] (1) Take the dried Tongjingcao powder, add distilled water according to the material-to-liquid ratio of 1:40 to 1:120, extract in a water bath at a constant temperature of 95°C for 140 to 180 minutes, and centrifuge to remove the precipitate to obtain a crude extract;

[0040] (2) Concentrate the crude extract obtained in step (1) to obtain a concentrated solution, measure the volume of the concentrated solution, add ethanol twice the volume of the concentrated solution, store in a refrigerator at 4°C for more than 15 hours, centrifuge at 5000r / min for 5min, take the precipitate and dry it Obtain first grade crude extract;

[0041] (3) Continue to add ethanol with 2 to 3 times the volume of the concentrated solution described in step (2) to the supernatant obtained by centrifugation in step (2), place it in a refrigerator at 4°C for more than 15 hours, centrifuge at 5000r / min for...

Embodiment 1

[0043] Example 1 Response Surface Method Optimizing the Extraction Process of Tongjingcao Polysaccharide

[0044] Experimental method: A single factor experiment was used to study the influence of three factors, liquid-to-solid ratio, extraction temperature and extraction time, on the extraction rate of polysaccharides from Tongjingcao. The specific experiment is to investigate the other factor under the experimental conditions of fixing two factors, so as to obtain the influence of this factor on the extraction rate of T. japonicus polysaccharide at different experimental levels.

[0045] In order to optimize the extraction conditions of T. japonicus polysaccharides, orthogonal experiments and response surface experiments were used to take liquid-to-material ratio, extraction temperature and extraction time as experimental factors. Weighed 1 g of T. japonicus powder and arranged according to the orthogonal table and Box-Behnken scheme. experiment. Use distilled water to extr...

Embodiment 2

[0063] Embodiment 2 through the separation and purification of herbaceous polysaccharides, comprising the following steps:

[0064] (1) Take 150g of dried Tongjingcao powder, add it to 6000mL of distilled water, extract in a constant temperature water bath at 95°C for 155min, centrifuge at 5000r / min for 5min, remove the precipitate, and obtain a crude extract;

[0065] (2) Concentrate the extract obtained in step (1) to 120mL, add 240mL of absolute ethanol, place in a refrigerator at 4°C for more than 15h, centrifuge at 5000r / min for 5min, take the precipitate and dry it to obtain a primary crude extract;

[0066] (3) Add 240 mL of absolute ethanol to the supernatant obtained after centrifugation in step (2), place in the refrigerator at 4°C for more than 15 hours, centrifuge at 5000 r / min for 5 minutes, and obtain a secondary crude extract after precipitation and drying;

[0067] (4) Get 300 mg of the secondary crude extract obtained in step (3), dissolve it in 15ml distilled...

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Abstract

The invention discloses aleuritopteris argentea polysaccharide and a preparation method and application thereof. According to the preparation method, an extracting solution of aleuritopteris argentea powder is subjected to fractional precipitation through ethyl alcohol, a first-stage crude extract and a second-stage crude extract with different sugar components are obtained, the aleuritopteris argentea polysaccharide is simply and effectively subjected to primary separation, the second-stage crude extract is directly purified, the aleuritopteris argentea polysaccharide with the anti-inflammation and anti-oxidation effects is obtained, and the steps of the method are simple. The aleuritopteris argentea polysaccharide prepared by the method is clear in component and consists of homogeneous polysaccharide with the weight-average molecular weight of 480KDa, and monosaccharide consists of glucose, galactose, mannose, xylose and arabinose. The aleuritopteris argentea polysaccharide prepared by the method has obvious anti-inflammatory effect and antioxidant activity, and is non-toxic to cells. Through scavenging activity research on superoxide free radicals, hydroxyl free radicals and DPPH free radicals, the anti-oxidation effect of the aleuritopteris argentea polysaccharide is superior to that of vitamin c, and the aleuritopteris argentea polysaccharide can be used for preparing anti-inflammatory and anti-oxidation preparations for cosmetics and medicines.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a polysaccharide of Tongjing grass and its preparation method and application. The obtained Tongjing grass polysaccharide can be used to prepare anti-inflammatory and antioxidant preparations, and is applied to cosmetics and pharmaceutical products. Background technique [0002] Tongjingcao (Aleuritopteris argentea) is the whole herb of Pteridaceae in my country. It is mainly distributed in Shandong, Inner Mongolia, Hebei, Yunnan and other provinces and regions in China, and is rich in resources. Tongjingcao is widely used in ethnic minority areas in China, such as Koreans, Tibetans, and Mongolians. It has extremely high medicinal value. According to reports, Mongolian medicine uses it to treat irregular menstruation, cough, wound suppuration, and coma. [0003] At present, there are few researches on T. syringae at home and abroad, mainly focusing on the chemical co...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K8/73A61P29/00A61P39/06A61Q19/08A61Q19/00A61K36/12
CPCC08B37/0003A61K31/715A61K8/73A61P29/00A61P39/06A61Q19/08A61Q19/00A61K36/12A61K2236/331A61K2236/39A61K2236/53A61K2236/51Y02A50/30
Inventor 王昭晶
Owner HUAQIAO UNIVERSITY
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