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Composite repair gel containing umbilical cord mesenchymal stem cells and platelet-rich plasma for injection

A kind of mesenchymal stem cell and compound repair technology, which is applied in the direction of drug combination, medical preparations with non-active ingredients, non-effective ingredients of polymer compounds, etc., can solve the problems of easy degradation, single type, and short half-life of growth factors, etc., to prevent Effects of anoikis, promotion of tissue regeneration, and prolongation of action time

Active Publication Date: 2021-02-26
中科细胞科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after platelet-rich plasma is activated, more than 90% of the pre-synthesized growth factors will be released within 1 hour, and the growth factors have a short half-life and are easily degraded in the body, so they cannot continue to play a role in promoting angiogenesis locally
Different stages of the skin repair process require different types of growth factors to participate, and cells have different requirements for the types and concentrations of various growth factors at different stages of growth, so the application of platelet-rich plasma or stem cells alone cannot meet the needs of skin repair. However, the current release of growth factors in tissue engineering is mostly limited to a single type and a single concentration, and the results are often unsatisfactory, which is difficult to meet the needs of facial beauty

Method used

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  • Composite repair gel containing umbilical cord mesenchymal stem cells and platelet-rich plasma for injection
  • Composite repair gel containing umbilical cord mesenchymal stem cells and platelet-rich plasma for injection
  • Composite repair gel containing umbilical cord mesenchymal stem cells and platelet-rich plasma for injection

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Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1 The cultivation and detection of umbilical cord mesenchymal stem cells

[0043] 1. Experimental steps

[0044] 1. Umbilical cord mesenchymal stem cell culture

[0045] The umbilical cords of newborns with a length of 15-20 cm were taken, and the tests of HBV antigen, anti-HCV antibody, anti-HIV antibody, anti-Treponema pallidum antibody, mycoplasma, and anti-CMV antibody were all negative. Place the umbilical cord of the newborn in the umbilical cord protection solution, within 6 hours from the collection to the preparation of the umbilical cord, wash it with PBS under sterile conditions until there is no blood, cut it longitudinally, remove the blood vessels and cut it into pieces to a size of 1mm×1mm×1mm Tissue block, add 0.5ml umbilical cord tissue suspension to 75cm 2 Add 10ml serum-free complete medium to the culture flask at the same time, put it in 37℃, 5%CO 2 cultured in an incubator. Change the medium in half on the 3rd day and the full volume ...

Embodiment 2

[0052] The preparation of embodiment 2 umbilical cord mesenchymal stem cell gel

[0053] 1. Preparation of silk fibroin-hyaluronic acid gel

[0054] (1) Degumming of mulberry silk fiber: put 200g of mulberry silk fiber in 5L of 0.6% sodium carbonate solution dissolved in deionized water, cook three times at 98-100°C, each time for 30-45min, each treatment Then rinse with deionized water to remove the sericin in the raw silk, and then loosen the degummed silk and dry it in an oven at 60°C to obtain silk fiber.

[0055] (2) Preparation of regenerated silk fibroin solution: preparation of CaCl 2 、C 2 h 5 OH, H 2 Ternary solution of O (molar ratio 1:2:8). Slowly stir and dissolve the dried silk fiber in the ternary solution at 72°C according to the bath ratio of 1:10 (g:mL). The silk fiber can be chopped in advance and the dissolution time is 1 hour to obtain silk and A mixed solution of a ternary solution. After the mixed solution is cooled, put it into a dialysis bag, the...

Embodiment 3

[0060] Embodiment 3 Preparation of platelet-rich plasma (PRP) gel

[0061] Take 10ml whole blood from autologous venous blood, put it into two 10mL anticoagulant tubes containing 1ml compound sodium citrate, shake well, centrifuge at 200g centrifugal force for 10min, absorb all the supernatant to 2mm below the interface and transfer to another centrifuge tube After the solution is balanced, centrifuge at a centrifugal force of 200g for 10 minutes, absorb 3 / 4 of the supernatant and discard it, and the rest is platelet-rich plasma. Mix platelet-rich plasma and activator (1 mL of 10% calcium chloride and 1000 U thrombin) at a ratio of 9:1 and shake well, and let it stand for about 2 minutes to form a platelet-rich plasma gel.

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Abstract

The invention discloses a composite repair gel containing umbilical cord mesenchymal stem cells and platelet-rich plasma for injection. According to the repair gel, mesenchymal stem cell gel and platelet-rich plasma gel are mixed to form the composite repair gel for injection, and the composite repair gel can improve the survival rate of stem cells, prolong the action time of bioactive molecules in vivo and realize local medication; and active substances secreted by the umbilical cord mesenchymal stem cells can recruit endogenous stem cells, promote tissue regeneration and have a great improvement effect on wrinkles and spots of human face skin.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a composite repair gel for injection containing umbilical cord mesenchymal stem cells-platelet-rich plasma. Background technique [0002] Changes in facial skin texture, the appearance of wrinkles and fissures, and loss of cartilage and bone are the root causes of facial aging. Therefore, facial rejuvenation focuses on restoring the lost soft tissue volume in the face as well as improving skin texture and removing wrinkles. [0003] Current treatments for facial rejuvenation include surgical and non-surgical treatments. Surgical treatment can quickly resolve the loosening of the upper and lower eyelid skin or the displacement of the subcutaneous tissue caused by the loosening of the supporting structure, but it has no advantage in improving the skin texture, and the trauma is large, the recovery time is long, and infection and scarring may even occur. complication. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/42A61K47/36A61K35/28A61P17/00
CPCA61K9/0019A61K9/06A61K47/42A61K47/36A61K35/28A61P17/00Y02A50/30
Inventor 唐忆琳钟伟珍陈巧林
Owner 中科细胞科技(广州)有限公司
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