Black material ferment, preparation method thereof and use thereof for increasing antioxidant activity and protecting eyes
A technology of fermented products and manufacturing methods, which is applied in the direction of drug combinations, pharmaceutical formulas, plant raw materials, etc., can solve problems such as blurred vision and eye fatigue, and achieve the effects of increasing flexibility, preventing injuries, and improving blurred vision
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[0038] The present invention provides a black material fermented product and its preparation method and its use for increasing antioxidant activity and eye protection. The black material fermented product of the present invention is a mixed juice of black sesame, black date juice, and black currant juice. Yeast, Lactobacillus, and Acetobacter are obtained by sequentially performing a three-stage fermentation, wherein the yeast is a strain of BCRC20271, the Lactobacillus is a strain of preservation number DSM 33108, and the Acetobacter is a strain of BCRC12324. The black material fermented product of the present invention can effectively increase antioxidant activity, prevent retinal cells from being damaged by blue light, increase eye adjustment flexibility, improve eye fatigue, and improve blurred vision.
[0039] At the same time, the composition for increasing antioxidant activity and protecting eyes of the present invention may also include an effective amount of fermented ...
Embodiment 1
[0041] Embodiment 1 The preparation method of the black material fermented product of the present invention
[0042] In one embodiment of the present invention, black sesame seeds, black date juice, and black currant juice are uniformly mixed in a weight ratio of 1:2-5:1-3, and heated at 95°C for 1.5 hours, the mixed solution Cool to room temperature for subsequent three-stage fermentation. First, implant 8x10 9 -1.2x10 10 The saccharomyces cerevisiae of cfu / g (Saccharomycescerevisiae, purchased from Biological Resources Conservation and Research Center, Taiwan, China, numbering is BCRC20271), the number of bacteria is preferably 1×10 10 cfu / g, pre-fermentation at room temperature for 20-24 hours, the actual time varies depending on the state of fermentation. Then directly into the 8x10 10 -1.2x10 11 cfu / g of Lactobacillus (Lactobacillus plantarum experiment number TCI028, preserved in DSMZ-German Microorganism Collection Center, preservation number DSM 33108), the number...
Embodiment 2
[0043] Embodiment 2 The superoxide dismutase (SOD) activity promotion of the fermented product of black material of the present invention
[0044] One embodiment of the present invention is to test the three-stage fermentation steps of the present invention to increase the activity of superoxide dismutase (Superoxide dismutase, SOD) in the black material fermented product, to inhibit gallicol (1,2,3-Trihydroxybenzene, Pyrogallol, purity >99.7%) for the quantification of SOD activity from the efficiency of the oxidation reaction. Among them, SOD is an enzyme that can catalyze superoxide through disproportionation reaction and convert it into oxygen and hydrogen peroxide. Oxidant, protects cells exposed to oxygen.
[0045] First, configure A solution and B solution for determination, wherein A solution is a mixture of 1.2114g of Tris(hydroxymethyl)aminomethane, Tris) and 37.2mg of ethylenediaminetetraacetic acid (EDTA-2Na) Dissolve in 62.4mL of hydrochloric acid (HCl) solution...
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