Adenovirus vector bivalent vaccine for simultaneously preventing avian influenza H5 and H9 subtypes and preparation method of adenovirus vector bivalent vaccine

Abstract

The invention provides an adenovirus vector bivalent vaccine for simultaneously preventing avian influenza H5 and H9 subtypes and a preparation method of the adenovirus vector bivalent vaccine. Hemagglutinin antigens of the avian influenza H5 subtype and H9 subtype are used as core antigens to synthesize a fusion gene H5H9HA, an adenovirus is used as a vector, the fusion gene H5H9HA is cloned to an adenovirus shuttle vector pDC315, then the fusion gene H5H9HA and skeleton plasmids pBHGlox (delta) E1 and 3Cre are co-transfected into 293 cells to complete recombinant adenovirus production; and then through amplification and purification, the high-purity recombinant adenovirus rAd-H5H9HA carrying the hemagglutinin antigens is obtained, and through freeze-drying and packaging post-treatment, an adenovirus vector bivalent vaccine capable of preventing avian influenza H5 and H9 subtypes at the same time is obtained. The vaccine can induce a high-level H5 subtype specific antibody and a high-level H9 subtype specific antibody, the level of the antibodies is consistent with that of an existing commercial totivirus inactivated vaccine and exceeds the immune standard specified by China, andthe vaccine has huge application prospects and popularization value in the field of avian influenza immunoprophylaxis.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to an adenovirus vector bivalent vaccine for simultaneously preventing avian influenza H5 and H9 subtypes and a preparation method thereof. Background technique [0002] Avian influenza is an acute, virulent, and contagious respiratory infectious disease of poultry caused by type A influenza virus. Avian influenza can be divided into highly pathogenic avian influenza and low pathogenic avian influenza. Highly pathogenic avian influenza has become an endemic disease in some Asian countries, and is listed as a "notifiable animal disease" by the World Organization for Animal Health (OIE), and is also listed as a first-class animal infectious disease in my country. Avian influenza can infect not only birds, but also humans and other mammals, so the prevention and control of avian influenza has public health significance. [0003] According to the difference of AIV hemagglut...

AI Technical Summary

Problems solved by technology

However, the disadvantage of this vaccine is that it can only be specifically immunized against one avian influenza H7 subtype virus, has a single function, and cannot produce immune protection against subtypes of avian influenza infections such as H5 and H9; according to authoritative epidemiological According to the scientific survey, the two subtypes of avian influenza that are most prevalent in domestic chicken flocks and cause the greatest harm are H5 subtype and H9 subtype, and relatively speaking, H7 subtype is more harmful than H5 subtype in chicken flocks. Much milder than the H9 subtype

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