Fermentation production process of lactobacillus fermentum

Abstract

The invention provides a fermentation production process of lactobacillus fermentum, which comprises the following steps: respectively and independently culturing lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus plantarum and bifidobacterium for 24-36 hours by using a seed culture medium to prepare a seed solution; respectively transferring the lactobacillus acidophilus seed solution, the lactobacillus rhamnosus seed solution, the lactobacillus plantarum seed solution and the bifidobacterium seed solution with the mass fraction of 3-4% into a basic culture medium, and carryingout closed culture for 10-16 hours; and inoculating into a fermentation culture medium according to the inoculum size of 4-5%, carrying out sealed fermentation for 8-12 hours, centrifuging, collecting bacterial sludge, adding a freeze-drying protective agent into the bacterial sludge, and carrying out freeze-drying to obtain freeze-dried powder. Within the same fermentation time, the fermentationproduction process provided by the invention simultaneously ferment lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus plantarum and bifidobacterium, so that the proliferation speed ofthe strain is higher, and meanwhile, the freeze-dried powder prepared from a fermented product of the lactobacillus acidophilus, the lactobacillus rhamnosus, the lactobacillus plantarum and the bifidobacterium is also higher in bacterial activity rate and smaller in strain loss.

Description

technical field [0001] The invention relates to biotechnology, in particular to a fermentation production process of Lactobacillus fermentum. Background technique [0002] Lactobacillus fermentum belongs to the genus Lactobacillus in the Lactobacillus family, is Gram-positive, and is facultatively anaerobic. The strains are rod-shaped, with a diameter of 0.5-0.9 μm, and the difference in length is relatively large. Most of them grow singly, and some appear in pairs. Colonies approximately 1 mm in diameter when grown on MRS agar medium, shiny, dome-shaped, opaque, and appear viscous when touched with an inoculating loop; non-spore-forming, catalase-negative, non-motile, able to utilize glucose , galactose, maltose, sucrose, lactose, melibiose, fructose, ribose, trehalose, raffinose, mannose and L-arabinose produce acid; catalase negative, gelatin liquefaction negative, hydrogen sulfide test negative, starch hydrolysis Negative, negative for ammonia production, negative for ...

AI Technical Summary

Problems solved by technology

[0003] But ordinary Lactobacillus fermentum can only survive in a relatively restricted environment, and its vitality is easily affected by the environment. In the existing Lactobacillus products, the number of viable Lactobacillus is usually not high, and the storage conditions are harsh and the stability is poor. , so it is necessary to develop a lactobacillus fermentation process with high bacterial viability and good stability