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A kind of anti-new coronavirus antibody and its preparation method and application

A coronavirus and a new type of technology, applied in the field of anti-new coronavirus antibodies and their preparation, can solve the problems of limited auxiliary role in group virus prevention and control, inability to achieve specific killing of pathogens, and high degree of dependence on the external environment, which is not easy to achieve. Allergy, wide range of protection, low-dose effect

Active Publication Date: 2021-03-26
CHENGDU YUKANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical killing method is to kill viruses by using broad-spectrum chemical agents that kill bacteria and viruses, such as 84 disinfectant, medical alcohol, etc. Chemical agents generally have certain toxicity to the human body, so they are mostly used as environmental antivirus. However, it cannot be directly applied to the surface of sensitive organs of the human body, such as eyes, ears, mouth, nose, etc. Some chemical agents with strong chemical toxicity cannot even have excessive contact with human skin, so the protective effect on the human body is very limited; while the physical killing method requires high temperature Viruses are killed by physical means such as high pressure, ultraviolet rays, etc., but this method is highly dependent on the external environment, and it cannot be directly applied to the protection of the human body surface. Neither the chemical killing method nor the physical killing method can achieve pathogens Specific killing, large side effects, very limited auxiliary effect on the prevention and control of mass viruses

Method used

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  • A kind of anti-new coronavirus antibody and its preparation method and application
  • A kind of anti-new coronavirus antibody and its preparation method and application
  • A kind of anti-new coronavirus antibody and its preparation method and application

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Comparison scheme
Effect test

Embodiment 1

[0028] The present invention specifically provides an anti-new coronavirus antibody, which is characterized in that the antibody is a high-titer specific antibody extracted from chicken egg yolk, and the chicken egg yolk is mainly obtained from eggs produced by immunized hens. Obtained, the immunized hen is a healthy hen injected with a new coronavirus vaccine.

[0029] Wherein, the novel coronavirus vaccine is mainly prepared from recombinantly expressed novel coronavirus (SARS-CoV-2) S antigen or virus-inactivated novel coronavirus (SARS-CoV-2) antigen.

[0030] Its specific preparation method comprises the following steps:

[0031] 1. Antibody preparation:

[0032] S1. Vaccine preparation:

[0033] Take the recombinantly expressed new coronavirus (SARS-CoV-2) S antigen or the virus-inactivated new coronavirus (SARS-CoV-2) antigen, and add the antigen to Freund's complete adjuvant at a ratio of 1:1 (initial immunization) or Freund's incomplete adjuvant (boosting immunizat...

Embodiment 2

[0056] This embodiment compares the yolk antibody production situation of two groups of different antigen immunization doses, and the preferred antigen immunization dose is specifically shown in the following table:

[0057] Table 1 The rise and fall of egg yolk antibody titers with different antigen immunization doses

[0058]

[0059] (NT: As the hen did not lay eggs after immunization, no detection was performed.)

[0060] It can be seen from the above table that the 5 μg / feather antigen dose group is preferred, and the yolk antibody titers produced in this dose group after 6, 8, and 10 weeks of primary vaccination are not lower than the 10 μg / feather antigen dose group.

Embodiment 3

[0062] In this embodiment, in order to verify the anti-new coronavirus yolk antibody neutralizing virus performance, the following tests were carried out:

[0063] Take 105~107 TCID50 / ml of the new coronavirus liquid and mix them with the new coronavirus egg yolk antibody samples with concentrations of 1mg / ml, 2mg / ml, and 3mg / ml according to the volume ratio of 1:1, and mix them evenly, and neutralize at 25~37℃ for 1 After ~2 hours, add it to a 96-well plate containing cells, incubate in a carbon dioxide incubator at 33-35°C, and observe cell changes daily under a microscope.

[0064] Under the condition of 25-37 ℃, the concentration of 2mg / ml and 3mg / ml of the new coronavirus egg yolk antibody was mixed with the new coronavirus liquid of 105~107TCID50 / ml, and the cells had no pathological changes. As a positive control, the novel coronavirus liquid can cause pathological changes in cells.

[0065] It can be seen from the test results that the anti-new coronavirus IgY prepare...

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Abstract

The invention discloses an antibody against novel coronavirus. The antibody is a high-titer specific antibody extracted from chicken egg yolk. The chicken egg yolk is mainly obtained from eggs produced by immunized hens. The immune The hens are healthy hens injected with the new coronavirus vaccine. The invention also discloses an auxiliary drug for the prevention and control of novel coronavirus with the anti-new coronavirus antibody as the main component. It can be used for the prevention and blocking of new coronavirus infection, as well as the adjuvant treatment of early infection, and can be used for group virus prevention Control adjuvant medication.

Description

technical field [0001] The invention relates to the technical field of biological antibody immunization, and specifically refers to an anti-new coronavirus antibody and its preparation method and application. Background technique [0002] Coronaviruses belong to the order Nidovirales, the family Coronaviridae, and the genus Coronavirus. They are important pathogens of many domestic animals, pets, and human diseases, causing a variety of acute and chronic diseases. The Ninth International Committee on Virological Classification reported that Coronaviridae was divided into three genera namely α, β and γ genera. The novel coronavirus belongs to the Sarbecovirus subgenus of betacoronaviruses. [0003] Novel coronavirus pneumonia is a new infectious disease, and the population has no immunity and is generally susceptible. Novel coronavirus-infected pneumonia can occur in people with low immune function and normal immune function, and it has a certain relationship with the amoun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/02C07K1/36C07K1/34C07K1/30A61K39/42A61K9/08A61P31/14
CPCA61K9/08A61K2039/505A61K2039/542A61K2039/543A61K2039/544A61P31/14C07K16/02C07K16/10C07K2317/11C07K2317/76
Inventor 林小军杨鹏辉武婕廖明王欢朱玲雷雨胡方焱叶向阳叶琳
Owner CHENGDU YUKANG BIOTECHNOLOGY CO LTD
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