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Application of 3,5-dichloroanthranilic acid in induction of resistance of arabidopsis thaliana to gray mold and method of 3,5-dichloroanthranilic acid

An anthranilic acid, Arabidopsis technology, applied in 3. The compound is in the field of plant resistance induction, which can solve the problems of high cost of Botrytis cinerea, poor water solubility of the inducer, and environmental pollution, so as to achieve no secondary pollution and water solubility. The effect of good sex and low dosage

Active Publication Date: 2020-08-07
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide the application of 3,5-dichloroanthranilic acid to induce the resistance of Arabidopsis to Botrytis in order to solve the problems of high cost, environmental pollution and poor water solubility of the inducer in preventing and treating Botrytis thaliana at present. and its methods

Method used

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  • Application of 3,5-dichloroanthranilic acid in induction of resistance of arabidopsis thaliana to gray mold and method of 3,5-dichloroanthranilic acid
  • Application of 3,5-dichloroanthranilic acid in induction of resistance of arabidopsis thaliana to gray mold and method of 3,5-dichloroanthranilic acid
  • Application of 3,5-dichloroanthranilic acid in induction of resistance of arabidopsis thaliana to gray mold and method of 3,5-dichloroanthranilic acid

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Effect test

Embodiment 1

[0037] Embodiment one: the cultivation of Arabidopsis plant

[0038] The seeds of wild-type Arabidopsis (Col-0) were suspended in 0.1% agarose solution, vernalized at 4°C for 2-4 days at low temperature, and sown in mixed nutrient soil (vermiculite: plant ash: perlite = 6 :2:1), apply fertilizer, dilute the fertilizer and water according to the mass volume ratio l:1000, and cover with film for 2-3 days to facilitate the emergence of seedlings. The culture temperature is maintained at 22-24 °C, the relative humidity is 80%, and the light intensity is about 85 μmol·s -1 m -2 , the light time is 16 hours. Other moisture, insect pests and cultivation management are as usual. 4-week-old Arabidopsis plants were used for the experimental treatments.

Embodiment 2

[0039] Embodiment two: the processing of 3,5-dichloroanthranilic acid

[0040] Prepare 10 μM 3,5-dichloroanthranilic acid aqueous solution. The roots of Arabidopsis plants were treated with 10 μM 3,5-dichloroanthranilic acid aqueous solution. The Arabidopsis plants were irrigated with the same volume of water as the control group, and two days later, Botrytis cinerea was inoculated.

Embodiment 3

[0041] Embodiment three: the Arabidopsis thaliana obtained by the processing of embodiment two is inoculated with Botrytis cinerea

[0042] Use 2×V8 solid medium (36% V8 fruit juice, 0.2% CaCO 3 , 2% agar) to cultivate Botrytis cinerea at a temperature of 20-25°C. After the dish is full of spores, put the mycelium block into the resuspension solution (4% maltose, 1% tryptone) and vigorously vortex to release the spores, filter the spores with gauze, and adjust the spore concentration with a hemocytometer 2×10 5Spores / mL. When inoculating, spray the spore suspension evenly on the inoculated seedlings until most of the leaves are covered with fine mist droplets. The inoculated seedlings were placed at 24°C, the photoperiod was 12 hours light / 12 hours dark, and the light intensity was 200 μE·m -2 S -1 , Observe the incidence after 4 days of highly moisturizing film. The same volume of resuspension was sprayed on Arabidopsis control plants as a control (MOCK).

[0043] as a...

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Abstract

The invention discloses an application of 3,5-dichloroanthranilic acid in induction resistance of arabidopsis thaliana to gray mold and a method of the 3,5-dichloroanthranilic acid, and belongs to thefield of induction of plant resistance by compounds. According to the method, after a 3,5-dichloroanthranilic acid aqueous solution is subjected to root irrigation or page spraying treatment on the cultivated arabidopsis thaliana of all ages for two days, botrytis cinerea is inoculated, and the result shows that arabidopsis thaliana seedlings treated with 3,5-dichloroanthranilic acid show that the resistance to botrytis cinerea is obviously improved; the 3,5-dichloroanthranilic acid induced arabidopsis thaliana has the advantages of being good in water solubility, small in use amount, safe, green, cheap, easy to obtain and the like when used for inducing the arabidopsis thaliana to generate resistance to gray mold.

Description

technical field [0001] The invention belongs to the field of compound-induced plant resistance, and relates to an application of a compound in plant-induced resistance and a method thereof, in particular to 3,5-dichloroanthranilic acid-induced Arabidopsis resistance to Botrytis cinerea applications and methods. Background technique [0002] 3,5-Dichloroanthranilic Acid (DCA), also known as 2-amino-3,5-dichlorobenzoic acid, is a white crystal powder with low toxicity and is often used as an intermediate in medicine and spices body. The chemical formula is C7H5Cl2NO2, the molecular weight is 206.026, the density is 1.607g / cm3, the melting point is 227-230°C (lit.), the boiling point is 344.4ºCat760mmHg, and the CAS registration number is 2789-92-6. [0003] When people study the growth and development of plants, they have used corn, tomato, pea, rice, barley, petunia and snapdragon as model gene systems, but the results are not satisfactory. Until 2000, Arabidopsis thaliana...

Claims

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Application Information

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IPC IPC(8): A01N37/44A01P21/00A01P3/00A01G7/06
CPCA01N37/44A01G7/06
Inventor 张译匀陆玲悦张慧娟
Owner TAIZHOU UNIV
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