Microbial preparation with formaldehyde removal effect as well as preparation method and application thereof

A microbial preparation and formaldehyde technology, applied in the field of microbial preparations, can solve the problems of nail softening, poor effect, easy fatigue, etc., and achieve the effect of good removal effect, wide energy source, and strong adaptability

Inactive Publication Date: 2020-04-21
SHENZHEN HE MIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when the nails are repeatedly stimulated by formaldehyde, it will also cause the nails to soften, change color, etc.
[0005] (2) Toxic effects: High concentration of formaldehyde can cause dizziness, insomnia, fatigue, distraction, abdominal pain, nausea, vomiting, general weakness, etc.; induce bronchial asthma and other respiratory diseases; stimulate the human body to produce IgG antibodies and The decrease in the proportion of T lymphocytes affects the immune system and leads to a decline in immunity; it can also cause menstrual disorders, infertility, and neonatal deformities, etc. Children and pregnant women are especially sensitive
[0006] (3) Carcinogenesis: formaldehyde is also a substance that is toxic to genes. Various animal experiments have proved that formaldehyde is both a carcinogen and a cancer accelerator, and may cause lung, nasopharynx, oral cavity, skin, colon and other tissues. organ cancer
[0009] Some existing microbial formaldehyde removal products have the defect that their functional strains cannot be obtained stably. For example, the invention patent application with the application number 201610923087.9 uses the combined fermentation of garbage fermentation liquid and activated sludge from the aeration tank of a polyoxymethylene plant to obtain dominant colonies. Large-scale application in industry is very limited
There are also some existing products whose functional bacteria are usually used alone or in combination with several strains that directly consume formaldehyde. They mainly use formaldehyde as an energy source, and for most environments that require formaldehyde removal, the formaldehyde is released continuously. Yes, in the middle and late stages of use, there are fewer and fewer viable bacteria, and the effect is getting worse and worse.

Method used

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  • Microbial preparation with formaldehyde removal effect as well as preparation method and application thereof
  • Microbial preparation with formaldehyde removal effect as well as preparation method and application thereof
  • Microbial preparation with formaldehyde removal effect as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1 Microbial preparation with formaldehyde removal function of the present invention and preparation method thereof

[0039] s1), each functional bacteria were obtained through pure culture

[0040] Halomonas, arsenic-oxidizing bacteria and Lactobacillus were activated according to conventional operations, and cultured in the culture medium, adjusted to pH 7.3, and cultured at 37°C for 24 hours; formaldehyde was added to the culture medium to reach a final mass concentration of 0.2% , and continue to cultivate for 36 hours, and the surviving colonies after the end of the cultivation are set aside.

[0041] The medium of the Halomonas is: LB liquid medium containing 3% NaCl by mass fraction; the medium of the arsenic-oxidizing bacteria is the LB medium; the medium of the Lactobacillus is the MRS medium.

[0042] s2), each functional bacterium obtained in step s1) is cultivated respectively

[0043] The clean Halomonas, arsenic-oxidizing bacteria and Lactobaci...

Embodiment 2

[0047] Example 2 The microbial preparation with formaldehyde removal function of the present invention and its preparation method

[0048] s1), each functional bacteria were obtained through pure culture

[0049] Halomonas, arsenic-oxidizing bacteria and Lactobacillus were respectively activated according to routine operations, and cultured in the culture medium, the pH was adjusted to 7.2, and cultured at 37°C for 30 hours.

[0050] The medium of the Halomonas is: LB liquid medium containing 8% NaCl by mass fraction; the medium of the arsenic-oxidizing bacteria is MSM medium; the medium of the Lactobacillus is LB medium.

[0051] s2), each functional bacterium obtained in step s1) is cultivated respectively

[0052] The clean Halomonas, arsenic-oxidizing bacteria and Lactobacillus in step s1) are picked out and inoculated in the culture medium respectively; the culture medium of each functional bacteria is the same as that of s1.

[0053] s3), mixed culture

[0054] The ha...

Embodiment 3

[0056] Embodiment 3 Microbial preparation with formaldehyde removal function of the present invention and preparation method thereof

[0057] s1), each functional bacteria were obtained through pure culture

[0058] Halomonas, arsenic-oxidizing bacteria and Lactobacillus were activated according to conventional operations, and cultured in the culture medium, the pH was adjusted to 7.4, and cultured at 37°C for 36 hours; formaldehyde was added to the culture medium to a final concentration of 0.5%. The culture was continued for 30 hours, and the surviving colonies were used after the culture was over.

[0059] The medium of the Halomonas is: LB liquid medium containing 12% NaCl by mass fraction; the medium of the arsenic-oxidizing bacteria is beef extract peptone medium; the medium of the Lactobacillus is RSMA medium .

[0060] s2), each functional bacterium obtained in step s1) is cultivated respectively

[0061] The Halomonas, Arsenic Oxidizing Bacteria and Lactobacillus t...

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Abstract

The invention relates to the technical field of microbial preparations, in particular to a microbial preparation with a formaldehyde removal effect as well as a preparation method and application thereof. The microbial preparation provided by the invention comprises functional bacteria, namely, halomonas, aliihoeflea and lactobacillus. The bacterial colony number ratio of the halomonas to the aliihoeflea to the lactobacillus is (30-45): (18-25): (9-20). The preparation method of the microbial preparation comprises the following steps: carrying out pure culture to obtain functional bacteria; respectively culturing the obtained functional bacteria; and carrying out mixed culture on the functional bacteria obtained by respective culture to obtain the microbial preparation. According to the microbial preparation, the raw materials are easy to obtain, the preparation process is easy for industrial large-scale popularization, the microbial preparation is prepared from a culture stock solution, a stable micro ecological system is formed, and the performance of functional bacteria and functional substances in the product can be maintained for a longer time.

Description

technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a microbial preparation with formaldehyde-removing effect and its preparation method and application. Background technique [0002] Formaldehyde, also known as formaldehyde, has a chemical formula of HCHO, a molecular weight of 30.0, and a slightly higher density than air. It is a colorless gas with a strong pungent odor at room temperature. It is chemically active and can react with various compounds. Formaldehyde has a low boiling point (-21°C), is volatile and flammable, and is easily soluble in water, alcohol and ether, and its dissolution process is an exothermic reaction. [0003] Formaldehyde, as a native toxin, can combine with protein, and its main hazards are as follows: [0004] (1) Irritant effect: Formaldehyde has a strong irritating effect on human skin and mucous membranes. After human contact, it will cause eye dryness, tearing, blurred vision, re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/225C12R1/245C12R1/01
CPCC12N1/20B01D53/84B01D53/72B01D2251/95Y02A50/20
Inventor 林大淇
Owner SHENZHEN HE MIN BIOTECH CO LTD
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