Living body nerve scaffold with central perfusion system and manufacturing method thereof

A nerve and living technology, applied in the field of the manufacture of living nerve scaffolds, can solve the problems of insufficient local blood supply, easy formation of glial scars, etc., and achieve the effects of reducing concentration, promoting proliferation and differentiation, and high cell density.

Active Publication Date: 2020-04-07
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of insufficient local blood supply and easy formation of glial scars after the existing living nerve stent is implanted in the body, the present invention provides a living nerve stent with a central perfusion system and its manufacturing method

Method used

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  • Living body nerve scaffold with central perfusion system and manufacturing method thereof
  • Living body nerve scaffold with central perfusion system and manufacturing method thereof
  • Living body nerve scaffold with central perfusion system and manufacturing method thereof

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Experimental program
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Effect test

Embodiment 1

[0050] This embodiment provides a living nerve support, such as figure 1 As shown, it includes nerve bundle 1 and endothelial cell layer 2. There is a perfusion channel 3 inside the nerve bundle, and the endothelial cell layer 2 is modified.

[0051] According to the phenomenon that nerve cells grow linearly into a bundle structure on a surface with a large curvature, the living nerve scaffold is formed by linearly growing neural stem cells SH-SY5Y in a hydrogel catheter.

Embodiment 2

[0053] This embodiment provides a mold for manufacturing the living nerve support according to the first aspect of the present invention, including: a first positioning plate 4 , a lower mounting plate 5 , an upper mounting plate 6 and a needle 9 .

[0054]There are two first positioning plates 4 , which are respectively provided with a certain number of first holes for installing the needles 9 .

[0055] The lower mounting plate 5 and the upper mounting plate 6 can be assembled together to form a certain number of lumens, and surfaces capable of cooperating with the first positioning plate are formed at both ends of the lumens. Thus, when the lower mounting plate 5 and the upper mounting plate 6 are placed between the two first positioning plates 4, the first hole is aligned with the axis of the lumen.

[0056] In a preferred embodiment, the lower mounting plate 5 has a first surface parallel to the lumen, and a certain number of first grooves are arranged on the first surfac...

Embodiment 3

[0063] This embodiment provides a method for manufacturing a living nerve scaffold as described in Embodiment 1 using the mold in Embodiment 2, and the specific implementation method is as follows.

[0064] First, a hydrogel conduit was fabricated to provide a three-dimensional linearized growth environment for neural stem cell lines.

[0065] The material of the hydrogel catheter is agarose, the outer diameter is 2 mm, and the lumen diameter is 1 mm. The manufacturing method of the agarose catheter is specifically to weigh 3g of agarose powder, dissolve it in 100ml of deionized water, add a magnetic stirrer in the beaker, and heat at 100°C and 120 rpm until the agarose solution boils , the boiling agarose solution was left to stand for 1 minute until the bubbles disappeared, poured into a mold, and left to stand until the agarose solution cooled to room temperature to obtain a structurally stable agarose catheter.

[0066] The mold as figure 2 As shown, it is composed of a...

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Abstract

The invention provides a living body nerve scaffold with a central perfusion system and a manufacturing method of the living body nerve scaffold. The living body nerve scaffold is manufactured by three-dimensionally culturing a nerve bundle in vitro with a lumen structure, modifying endothelial cells on the inner wall of the lumen of the nerve bundle, and manufacturing under the condition of perfusion culture. In the perfusion culture process, the shear stress of a fluid can improve the activity of endothelial cells, construction of vascularized nerve tracts is facilitated, and therefore localblood supply of the nerve tracts in the early stage after the nerve tracts are implanted into the body is improved. Meanwhile, the shear stress of the fluid can promote proliferation and differentiation of the neural stem cells, and perfusion culture can enable the nerve tracts to obtain more sufficient nutrient supply and reduce the concentration of metabolic wastes in a cell growth environment,so that the nerve tracts with higher cell density are obtained.

Description

technical field [0001] The invention relates to a manufacturing method of a living nerve support with a central perfusion system, belonging to the field of tissue engineering. Background technique [0002] At present, due to accidents, malignant diseases and other reasons, there are many patients with peripheral nerve injuries worldwide, and the number of new patients continues to increase at a rate of more than 1 million per year. The areas innervated by the injured nerves of patients with nerve injuries will Problems such as perception disorders, movement disorders, and nutritional disorders appear, which bring a lot of inconvenience to the lives of patients. Due to the weak self-repair ability of nerve tissue, research on reliable nerve repair technology has become a key issue in the field of neural tissue engineering. [0003] The current methods for nerve repair are mainly divided into two methods based on autologous nerve cell transplantation and methods based on biom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/50C12N5/071C12M3/00
CPCA61L27/3808A61L27/383A61L27/3895A61L27/50A61L2430/32C12M21/08C12M23/06C12M23/10C12N5/069C12N2502/088C12N2513/00
Inventor 石青魏子厚孙韬陈勰下田真吾黄强福田敏男
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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