Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent

A hydrogel and biological matrix technology, applied in biochemical equipment and methods, microbial-based methods, immobilized enzymes, etc., to achieve environmental friendliness, excellent adsorption effect, and easy promotion and use

Pending Publication Date: 2020-02-21
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the biomass-based high-performance dual-network hydrogel adsorbent for immobilized yeast

Method used

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  • Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent
  • Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent
  • Biomass-based hydrogel adsorbent of immobilized yeast as well as preparation method and application of biomass-based hydrogel adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] This embodiment provides a hydrogel, including tea residue and Pichia pichia cells.

[0034] The preparation method comprises the following steps:

[0035] (A) Preparation of Pichia pedoriasis sludge preparation.

[0036] (2) Preparation of tea dregs powder aqueous solution.

[0037] (3) Preparation and processing of tea residue hydrogel.

[0038] Step (1) Preparation of yeast sludge The specific steps of preparation are:

[0039] The strains were inoculated on the slant medium, cultured in a constant temperature incubator for 24 hours, then taken out, inoculated into YPD, and expanded on a shaker for 24 hours; then 1 mL of the bacterial liquid was added to YPD and YPD stressed with NaCl, respectively. After cultivating on a shaker for 24 hours, put the bacterial solution into 5mL centrifuge tubes, and centrifuge at 3700r for 5min to obtain the bacterial sludge. The number of bacteria in the bacterial sludge is 4×10 6 .

[0040] Step (2) The specific steps for the ...

Embodiment 2

[0046] Example 2: Validation of the hydrogel of Example 1

[0047] 1 SEM results

[0048] Take a small amount of unground TR / PAA hydrogel samples after freeze-drying, fix them on copper plates with conductive glue, spray gold on the surface of the samples, and observe the surface and Cross-sectional morphology to determine its microstructure. The swelling of the hydrogel made the internal structure contain a lot of water, and it was freeze-dried. The microstructure and surface morphology of the swollen TR / PAA xerogel were studied under scanning electron microscopy (SEM) (such as figure 1-d) It can be clearly seen that the TR / PAA xerogel has a three-dimensional porous network structure inside, and there are interconnected and irregular pores inside. According to the scale on the figure, it can be estimated that the pore diameter ranges from 100.0 to 200.0 μm, which belongs to large hole structure. Obviously, the functional groups in this loose and porous adsorbent can be com...

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PUM

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Abstract

The invention discloses biomass-based hydrogel of immobilized yeast and a preparation method of the biomass-based hydrogel. The hydrogel has a three-dimensional macroporous network structure, adsorption sites are fully exposed, water-soluble small molecules can be freely diffused, and the hydrogel is a dual-network hydrogel adsorbent with very high adsorption efficiency on metal ions in an aqueoussolution. According to the invention, microorganisms with an adsorption effect are immobilized into the gel adsorbent, so that the gel adsorbent can fully diffuse water-soluble small molecular substances, has an excellent adsorption effect on heavy metal ions in water, and is environment-friendly, low in cost and convenient to popularize and use.

Description

technical field [0001] The invention relates to the preparation of a biomass-based gel of immobilized yeast and its application as a heavy metal adsorbent, and belongs to the technical fields of environmental functional materials and water pollution treatment. Background technique [0002] Due to the environmental persistence, non-biodegradability, easy bioaccumulation and lethal toxicity of heavy metal pollution in water bodies, it has serious toxic effects on humans and organisms even at relatively low concentrations. In recent years, a variety of treatment technologies have been used to remove toxic metal ion pollutants in wastewater, such as ion exchange, filtration, chemical precipitation, reverse osmosis, redox, electrocoagulation, adsorption, etc. Compared with other methods, the adsorption method has the advantages of high efficiency, low cost, easy operation and implementation, and no secondary pollution. Therefore, the preparation of a low-cost, high-efficiency, a...

Claims

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Application Information

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IPC IPC(8): B01J20/28B01J20/22C12N11/12C12N11/087C12N11/04C08F289/00C08F220/06C08F222/38C02F3/34C12R1/84C02F101/20
CPCB01J20/28047B01J20/22C12N11/12C12N11/08C12N11/04C08F289/00C02F3/34C02F3/347C02F2101/20
Inventor 汪东风张帅中徐莹陈鹏李钰金
Owner OCEAN UNIV OF CHINA
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