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Preparation method for natural basin-based bionic structure bone scaffold

A natural protein and structural bone technology, which is applied in the field of preparation of natural protein-based biomimetic structural bone scaffolds, can solve the problem of complete repair and reconstruction of defect areas that are difficult to achieve osseointegration, unfavorable cell communication and information transmission, and cell growth and migration. Limitation and other issues, to achieve the effect of low price, huge development potential and broad market prospects

Active Publication Date: 2019-03-01
NANTONG TEXTILE & SILK IND TECH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the common problems of these materials are that the growth of new bone is limited by the local microenvironment, and the crawling replacement is slow. When treating large-scale bone defects, it is difficult to achieve the integration of the scaffold and bone and the complete repair and reconstruction of the defect area.
The repair and regeneration of bone defects requires a suitable external microenvironment. If the internal framework structure of the material cannot simulate the network structure formed by the extracellular matrix of autologous bone, it is not conducive to the communication and information transmission between cells, and the growth and migration of cells involved in bone repair. Limited, difficult to achieve osseointegration

Method used

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  • Preparation method for natural basin-based bionic structure bone scaffold
  • Preparation method for natural basin-based bionic structure bone scaffold
  • Preparation method for natural basin-based bionic structure bone scaffold

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preparation example Construction

[0035] The preparation method of the above-mentioned composite porous material, the steps are as follows:

[0036] (1) Preparation of wool keratin by ion precipitation method;

[0037] (2) Preparation of silk fibroin by high-pressure boiling method;

[0038] (3) Two natural protein solutions were mixed and stirred to prepare natural protein-based biomimetic structural bone scaffolds.

[0039] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with specific embodiments and accompanying drawings.

[0040] A method for preparing a natural protein-based biomimetic structure bone scaffold, comprising:

[0041] Step 1, preparation of wool keratin by ion precipitation method:

[0042] The raw wool was immersed in a 2% NaOH solution in a constant temperature water bath. After the wool fibers were completely dissolved, the hydrolyzed solution was kep...

Embodiment 1

[0051] This implementation case shows a preparation method of a natural protein-based biomimetic structure bone scaffold, including:

[0052] (1) Alkaline method to prepare wool protein: use 1%~5% NaOH solution to dissolve a certain amount of wool fiber, filter it, and then use a dialysis bag with a molecular weight cut-off of 2000Da~20000Da for dialysis for 1~5 days , after concentration of a 15% polyethylene glycol (PEG) solution with a molecular weight of 20,000 for 12-24 hours, a wool protein solution with a mass fraction of 3-10% is obtained.

[0053] (2) Preparation of silk fibroin by high-pressure water boiling: degumming and dissolving natural silk, dialysis and centrifugation according to the method in step (1), and concentration of 15% PEG solution with a molecular weight of 20,000 for 6-12 hours to obtain The mass fraction is 10-20% silk fibroin solution.

[0054] (3) Mixing method of two natural protein solutions: Measure a certain amount of silk fibroin solution ...

Embodiment 2

[0057] This implementation case shows a preparation method of a natural protein-based biomimetic structure bone scaffold, including:

[0058] (1) Wool protein was prepared by reduction method: 7M urea, 0.08M Na 2 S and 0.8g sodium dodecyl sulfate (SDS) were prepared into 100 mL solution, then 5g of wool fiber was added, and the water bath temperature was 50°C for 8h water bath treatment, followed by filtration, and then carried out with a dialysis bag with a molecular weight cut-off of 2000Da~20000Da Dialysis, dialysis for 1-5 days, after concentration of 15% polyethylene glycol (PEG) solution with a molecular weight of 20,000 for 12-24 hours, a wool protein solution with a mass fraction of 3-10% was obtained.

[0059] (2) Preparation of silk fibroin by high-pressure water boiling: degumming and dissolving natural silk, dialysis and centrifugation according to the method in step (1), and concentration with a 15% PEG solution with a molecular weight of 20,000 to obtain a mass f...

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Abstract

The invention discloses a preparation method for a natural basin-based bionic structure bone scaffold. Silk protein and wool protein are taken as main raw materials, a silk protein solution and glycerinum are uniformly mixed according to the mass ratio of 60-120:20-60 and injected in a bone scaffold mold, a wool keratin solution is slowly injected into the mixed solution, and then through -40--20DEG C freezing and -72-5 DEG C vacuum freezing drying under 1.0*105-1.0 Pa, the silk protein / wool keratin bionic bone scaffold material is obtained. The prepared scaffold material is similar to the autogenous bone structure, the porosity rate reaches 70-95%, the hole diameter is uniform in distribution, the outer layer is compact, the hole diameter is smaller, the diameter range is 30-150 microns,the scaffold material has certain mechanical supporting performance, the diameter of inner holes is larger, the diameter range is 250-600 microns, and the scaffold material is suitable for migration,dependence and proliferation of cells.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of a natural protein-based biomimetic structure bone scaffold. Background technique [0002] Bone defect diseases caused by factors such as congenital defects or infection, trauma and tumor resection have been plagued by tens of millions of patients in the world. Traditional surgical treatment methods and radiotherapy and chemotherapy methods are usually difficult to effectively achieve the purpose of treatment. Organ transplantation is not only expensive and has few donor resources, but also has the risk of disease transmission and cross-infection, so it cannot be used as a routine treatment. In recent years, the development of tissue engineering materials has provided a new and effective way to treat such diseases. Among them, scaffold materials for bone defect repair have become one of the most clinically demanded biomedical mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/50A61L27/56
CPCA61L27/26A61L27/50A61L27/56A61L2430/02C08L89/04C08L89/00
Inventor 李刚田焰宽郑兆柱王晓沁
Owner NANTONG TEXTILE & SILK IND TECH RES INST
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