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Eye drops containing canine mesenchymal stem cell factor and preparation method thereof

A technology of stem cells and eye drops, applied in the field of veterinary medicine, can solve the problems of lack of very effective treatment plan, weakened vision, etc., and achieve the effect of accelerating the healing speed, promoting the repair of corneal damage, and being widely used.

Active Publication Date: 2019-02-05
维赛(佛山)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Canine corneal injury accounts for at least half of the serious eye injuries in dogs. If the canine corneal injury is not treated and repaired in time, the scar tissue formed will lead to greatly weakened vision. However, there is no very effective treatment for dog corneal injury repair at present Program

Method used

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  • Eye drops containing canine mesenchymal stem cell factor and preparation method thereof
  • Eye drops containing canine mesenchymal stem cell factor and preparation method thereof
  • Eye drops containing canine mesenchymal stem cell factor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An eye drop containing canine mesenchymal stem cell factor, which consists of canine adipose mesenchymal stem cell culture supernatant 48%, chloramphenicol 0.2%, sodium hyaluronate 0.45%, polyethylene glycol 400 5%, 1,2-propanediol 3.5% and the balance of normal saline.

[0024] Preparation:

[0025] 1) Take about 3ml of dog abdominal fat, wash it with PBS containing 10% double antibody, use sterilized ophthalmic scissors to cut the fat block to 1mm size, use type Ⅰ collagenase to enzymolyze it in a 37°C incubator for 40min, and sterilize Sieve through the bacterial single-cell filter membrane, centrifuge at 1200r / min for 5min to remove the supernatant, resuspend the cells with complete medium, add an appropriate amount of complete medium to a petri dish, and culture in a 5% carbon dioxide incubator at 37°C. Cells grow to a density of about 80% and pass down to 3-6 passages for identification. Take the culture supernatant and centrifuge it at 4000r / min for 20min, filte...

Embodiment 2

[0029] An eye drop containing canine mesenchymal stem cell factor, which consists of canine placental mesenchymal stem cell culture supernatant 40%, chloramphenicol 0.28%, sodium hyaluronate 0.25%, polyethylene glycol 600 8%, 1,2-propanediol 1.5% and the balance of normal saline.

[0030] As a further improvement of the above scheme, the supernatant of the canine mesenchymal stem cell culture medium is obtained by collecting the supernatant of canine mesenchymal stem cells through cell culture, centrifuging at 4000r / min for 20min, and filtering through a 0.22μm filter membrane.

[0031] Preparation:

[0032] 1) Take about 3ml of canine placenta, wash it with PBS containing 10% double antibody, use sterilized ophthalmic scissors to cut the placenta into 1mm size, enzymolyze it with type Ⅰ collagenase in a 37°C incubator for 40min, and sterilize Filter the single-cell filter membrane, centrifuge at 1200r / min for 5min to remove the supernatant, resuspend the cells with complete ...

Embodiment 3

[0036] An eye drop containing canine mesenchymal stem cell factor, which consists of 55% supernatant of canine amniotic fluid mesenchymal stem cell culture medium, 0.15% chloramphenicol, 0.65% sodium hyaluronate, polyethylene glycol 800 3%, 1,2-propanediol 5%, and the balance of normal saline.

[0037] As a further improvement of the above scheme, the supernatant of the canine mesenchymal stem cell culture medium is obtained by collecting the supernatant of canine mesenchymal stem cells through cell culture, centrifuging at 4000r / min for 20min, and filtering through a 0.22μm filter membrane.

[0038] Preparation:

[0039] 1) Take about 3ml of canine amniotic fluid, hydrolyze it with type I collagenase in a 37°C incubator for 40min, sieve through a sterilized single-cell filter membrane, centrifuge at 1200r / min for 5min to remove the supernatant, and resuspend the cells in complete medium. Add appropriate amount of complete medium to a petri dish and culture in a 5% carbon dio...

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Abstract

The invention discloses eye drops containing a canine mesenchymal stem cell factor. The eye drops are prepared from the following components in percentage by mass: 40-55% of canine mesenchymal stem cell culture solution supernatant, 0.15-0.28% of chloramphenicol, 0.25-0.65% of sodium hyaluronate, 3-8% of surfactant, 1.5-5% of cosurfactant and the balance of normal saline. In the invention, the canine mesenchymal stem cell culture solution supernatant contains a plurality of active cell factors and has the effects of promoting corneal injury repair, accelerating the healing of scar tissue, andeffectively reducing the generation of eye secretion after injury and the like; through the compound formula, the eye drops have a repairing function as well as excellent antibacterial ability, thus effectively lowers the risk of secondary infection, and solves the problems of incomplete repair such as watery eyes, eye secretion increase, photophobia and difficulty in scar tissue repair caused bysever injury of cornea of canine eyes. The invention also discloses a preparation method of the eye drops.

Description

technical field [0001] The invention relates to the field of veterinary medicine, in particular to an eye drop. Background technique [0002] Mesenchymal stem cells (Adipose mesenchymal stem cells, AD-MSC) are ubiquitous in fat, bone marrow, umbilical cord, placenta, amniotic fluid and other tissues or body fluids. They have a wide range of sources, are easy to separate, and have self-renewal, tissue damage repair, and immune regulation. It also has the ability to differentiate into epidermal cells. [0003] The secretion of mesenchymal stem cells cultured in vitro contains a large number of active ingredients, such as stem cell growth factor (SCF), fibroblast growth factor (FGF), epidermal growth factor (EGF), cell colony stimulating factor, collagen, etc. 80 A variety of active ingredients, which have a certain effect on promoting the recovery of corneal damage. [0004] Canine corneal injury accounts for at least half of the serious eye injuries in dogs. If the canine c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/08A61K47/10A61P27/02A61K31/165A61K31/728
CPCA61K47/10A61P27/02A61K9/0048A61K9/08A61K31/165A61K31/728A61K35/28A61K2300/00
Inventor 陈淑仪陈胜锋王丙云陈志胜林嘉颖姚家威
Owner 维赛(佛山)生物科技有限公司
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