Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A preparation method and application of berberine hydrochloride complex nanoparticles

A technology of berberine hydrochloride and complexes, applied in the field of preparation of berberine hydrochloride complex nanoparticles, can solve the problems of berberine hydrochloride difficult to absorb and low bioavailability

Inactive Publication Date: 2020-10-30
ZHENGZHOU UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method of berberine hydrochloride complex nanoparticles, which can effectively solve the problem that berberine hydrochloride is not easily absorbed in the body, resulting in low bioavailability The problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method and application of berberine hydrochloride complex nanoparticles
  • A preparation method and application of berberine hydrochloride complex nanoparticles
  • A preparation method and application of berberine hydrochloride complex nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: A preparation method of berberine hydrochloride complex nanoparticles of the present invention includes the following steps: (1) Preparation of berberine hydrochloride complex: 10 mg of berberine hydrochloride and 30 mg of soybean phospholipids are dissolved Place 3 mL of absolute ethanol in a round-bottomed flask. Stir in an oil bath at 30°C for 1 hour, and then rotate to evaporate to remove the organic solvent. A thin film, the first compound, is formed on the wall of the round-bottomed flask. The substance was dissolved in 2ml of chloroform and centrifuged at 4000r / min for 5min to remove excess and unreacted berberine hydrochloride to obtain the filtrate. The filtrate was rotary evaporated and dried in a vacuum drying oven to obtain the second compound;

[0019] (2) Dissolve 5 mg of the second compound and 75 mg of polyethylene glycol monomethyl ether-polycaprolactone in 2 ml of a mixed solvent made with a volume ratio of tetrahydrofuran and ethanol of 1:2, an...

Embodiment 2

[0020] Example 2: A preparation method of berberine hydrochloride complex nanoparticles of the present invention includes the following steps: (1) Preparation of berberine hydrochloride complex: 10 mg of berberine hydrochloride and 60 mg of soybean phospholipids are dissolved Place 20 mL of absolute ethanol in a round-bottomed flask. Stir in an oil bath at 60°C for 4h, and then rotate to evaporate to remove organic solvents. A thin film, the first compound, is formed on the wall of the round-bottomed flask. The substance was dissolved in 2ml of chloroform and centrifuged at 4000r / min for 5min to remove excess and unreacted berberine hydrochloride to obtain the filtrate. The filtrate was rotary evaporated and dried in a vacuum drying oven to obtain the second compound;

[0021] (2) Dissolve 5 mg of the second compound and 75 mg of polyethylene glycol monomethyl ether-polycaprolactone in 2 ml of a mixed solvent made with a volume ratio of tetrahydrofuran and ethanol of 1:2, and ultr...

Embodiment 3

[0022] Example 3: A preparation method of berberine hydrochloride complex nanoparticles, including the following steps: (1) Preparation of berberine hydrochloride complex: 10 mg of berberine hydrochloride and 60 mg of soybean phospholipids were dissolved in 5 mL In absolute ethanol, place it in a round-bottomed flask, stir for 3 h in an oil bath at 40 ℃, and then rotate to evaporate to remove the organic solvent. A thin film, the first compound, is formed on the wall of the round-bottomed flask. Dissolve in 2ml of chloroform, centrifuge at 4000r / min for 5min to remove excess and unreacted berberine hydrochloride to obtain the filtrate. The filtrate is rotary evaporated and dried in a vacuum drying oven to obtain the second compound;

[0023] (2) Dissolve 5 mg of the second compound and 75 mg of polyethylene glycol monomethyl ether-polycaprolactone in 2 ml of a mixed solvent made with a volume ratio of tetrahydrofuran and ethanol of 1:2, and ultrasonically assist the solvent to for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

A method for preparing berberine hydrochloride composite nanoparticles comprise the following steps: (1) preparing berberine hydrochloride composite nanoparticle; (2) preparing berberine hydrochloridecomposite nanoparticles; as the only active ingredient, the berberine hydrochloride composite nanoparticles prepared by the invention can inhibit MCF-7 cell drugs; the berberine hydrochloride composite nanoparticles prepared by the invention are used as the only active ingredient in preparing anti-staphylococcus aureus and anti-escherichia coli medicines. The above preparation method is simple, the prepared composite nanoparticles have uniform particle size, encapsulation efficiency is high, which can reduce the recognition and phagocytosis of reticuloendothelial phagocytosis system (RES), and has slow release effect, increase the long circulation of carrier in vivo, improve the biological activity of drug, and achieve high efficiency and low toxicity, which provides conditions for the clinical application of berberine hydrochloride, and has significant economic and social benefits.

Description

Technical field [0001] The invention relates to a medicine, in particular to a preparation method and application of berberine hydrochloride composite nanoparticles. Background technique [0002] Berberine hydrochloride is an isoquinoline alkaloid, which is distributed in many plants of the Berberiaceae family. At present, many studies have been carried out at home and abroad, and the research on hydrophobic drugs encapsulated by amphiphilic polymers has been carried out. Berberine hydrochloride is often used clinically in the treatment of bacterial diarrhea, acute and chronic ulcerative inflammation, and it also has certain effects on the digestive system, diabetes, and tumors. [0003] However, due to its low solubility, it is difficult to be absorbed in the body, resulting in low bioavailability, which limits the clinical application of berberine hydrochloride. [0004] Nano-drug carrier delivery system has injected new vitality into the research of pharmaceutical preparations. N...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/34A61K47/24A61K31/4375A61P31/04A61P35/00
CPCA61K9/5123A61K9/5146A61K31/4375A61P31/04A61P35/00Y02A50/30
Inventor 李爱荣王改利王瑞郭丽梁成林张振中
Owner ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com