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Culture medium for agrocybe cylindracea and preparation process of culture medium

A preparation process and culture medium technology, applied in the field of tea tree mushroom culture medium and its preparation, can solve the problems of slow growth of mycelium, high cost, damage, etc., and achieve the advantages of shortening the maturation period, increasing the output, and preventing soft rot. Effect

Inactive Publication Date: 2018-08-28
HUAIBEI ZHIHUAI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, it is generally used as main raw materials to prepare tea tree mushroom medium with miscellaneous wood chips, cottonseed hulls and corncobs. Growth is slow. In addition, during the fungus and fruiting stage, it is vulnerable to the damage of Neurospora mold and mushroom fly, which reduces the quality and yield of tea tree mushroom
[0003] The patent with the application number CN201711025129.8 discloses a tea tree mushroom culture medium containing compound nutrients and its preparation method. The acid regulates the optimum PH, which not only provides sufficient nutrients for the growth of tea tree mushrooms, but also prevents the occurrence of bacterial pollution and pests. However, the price of camellia oleifera is relatively expensive, and its main purpose is to produce tea seed oil, with camellia oleifera sawdust as the main The cost of cultivating tea tree mushrooms is high, which reduces the original value and additional output value of camellia oleifera trees.
The patent application number is CN201410039506.3, which discloses a culture medium and cultivation method of tea tree mushroom. The nutritional components and medicinal value of camellia and hemp have been improved, but in terms of pest control, they have not yet reached the standard of professional cultivation, which is easy to cause pathogenic infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1: After cutting 20% ​​rape straw, 30% rice straw, and 40% wheat straw into 3cm short branches, soak them in 3% clarified lime water for 15 hours, adjust the pH to 5.8, and the temperature is 25°C, and inoculate with 5wt% Composite microorganisms were fermented for 16 days under aerobic conditions to obtain modified straw;

[0027] The composite microorganism is formed by mixing white-rot fungi, Trichoderma, Pseudomonas, and Bacillus according to a weight ratio of 2:1.5:1:1;

[0028] Step 2: Stir potassium dihydrogen phosphate, polypeptide urea, and superphosphate evenly according to the weight ratio of 1:0.5:3 to prepare compound fertilizer, prepare 60mg / Lα-naphthaleneacetic acid, 2mg / L6-benzylaminopurine, 80mg / L L3-indoleacetic acid, 200mg / L gibberellin, and uniformly mixed to obtain a growth-promoting regulator, mixed 8 parts of 70% thiophanate-methyl wettable powder, 15 parts of 2-hydroxybenzamide, 1 part of 50% Phoxim emulsifiable concentrate, the anti-increa...

Embodiment 2

[0031] The operation is the same as in Example 1, the difference is that step 1: the percentage by weight of each component of the modified straw is 22%, 33%, and 45% successively, the length of the cut branch is 5cm, the concentration of clarified lime water is 4%, and the soaking time is 20h. The pH is 6.1, the temperature is 30°C, the inoculum amount of the compound microorganism is 6wt%, and the fermentation days are 18 days; step 2: the weight ratio of the components of the compound fertilizer is 1:0.6:3.5, and the concentration of each component of the growth-promoting regulator is 80mg / L, 3.5mg / L, 100mg / L, 250mg / L, the weight parts of each component of the antibiotic enhancer are 9 parts, 18 parts, 2 parts in turn; step 3: the weight parts of each component of tea tree mushroom culture medium are 40 parts in turn .

Embodiment 3

[0033] The operation is the same as in Example 1, the difference is that step 1: the percentage by weight of each component of the modified straw is 24%, 36%, and 50% successively, the length of the cut branch is 7cm, the concentration of clarified lime water is 5%, and the soaking time is 25h. The pH is 6.4, the temperature is 35°C, the inoculum amount of the compound microorganism is 7wt%, and the fermentation days are 20 days; step 2: the weight ratio of each component of the compound fertilizer is 1:0.7:4, and the concentration of each component of the growth-promoting regulator is 100mg / L, 5mg / L, 120mg / L, 300mg / L, the parts by weight of each component of the antibiotic enhancer are 10 parts, 21 parts, 3 parts successively; Step 3: the parts by weight of each component of tea tree mushroom medium are 50 parts, 120 parts, 30 parts, 45 parts, 70 parts, 25 parts, 2 parts, 0.5 parts, the cake fertilizer is soybean cake fertilizer, the homogenization rate and time are 2000-3000...

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PUM

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Abstract

The invention discloses a culture medium for agrocybe cylindracea. The culture medium comprises the following components in parts by weight: 30-50 parts of cottonseed hulls, 80-120 parts of modified straws, 20-30 parts of bran, 35-45 parts of furfural residues, 50-70 parts of cake fertilizer, 15-25 parts of compound fertilizer, 1-2 parts of growth regulator and 0.3-0.5 part of antifungal agent. The compound fertilizer is prepared by mixing monopotassium phosphate, polypeptide urea and calcium superphosphate according to the weight ratio of 1:(0.5-0.7):(3-4). A preparation process of the culture medium for agrocybe cylindracea is as follows: pretreating the straws for 15-25h by using clarified lime water, regulating the pH value to 5.8-6.4, carrying out aerobic fermentation for 16-20d, then, mixing the straws with raw materials including the furfural residues, the cake fertilizer, the growth regulator and the antifungal agent, and carrying out irradiation sterilization by using Co60-gamma rays. Cellulose and lignin are converted into monosaccharide or disaccharide, so that the actual utilization ratio of the culture medium is increased, and the produced culture medium contains abundant extracelluar enzymes and is capable of promoting the fission and differentiation of the agrocybe cylindracea, shortening the growth period, inhibiting pathogenic infection and improving the yieldand quality.

Description

technical field [0001] The invention belongs to the technical field of edible fungus production and cultivation, and in particular relates to a tea tree mushroom culture medium and a preparation process thereof. Background technique [0002] Tea Tree Mushroom, the scientific name is columnar mushroom, is a kind of edible and medicinal fungus, fresh and delicious, high in nutrition, high in protein, rich in vitamins, mineral elements and a variety of essential amino acids. The effect of the spleen. Compared with other kinds of edible fungi, the activity of extracellular cellulase and laccase of tea tree mushroom is lower, and its ability to degrade cellulose and lignin is weak. Wild tea tree mushroom only grows on camellia oleifera. In the prior art, it is generally used as main raw materials to prepare tea tree mushroom medium with miscellaneous wood chips, cottonseed hulls and corncobs. Growth is slow. In addition, in the stage of germination and fruiting, it is easy to b...

Claims

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Application Information

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IPC IPC(8): A01G18/20
CPCC05B1/02C05G3/00C05B7/00C05C9/00C05D3/02C05F5/002C05F5/006C05F11/00C05F11/10
Inventor 马敏戚晓楠
Owner HUAIBEI ZHIHUAI TECH CO LTD
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