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Gel polypeptide capable of being used for preparing hemostatic material

A technology of hemostatic materials and products, applied in the direction of medical preparations containing active ingredients, peptides, blood diseases, etc., can solve the problems of easy cracking and falling off, poor hemostatic effect, rebleeding, etc., and achieve good biocompatibility, The effect of good coagulation effect

Pending Publication Date: 2018-01-23
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, gelatin sponge has poor wound tissue adhesion, is easy to break and fall off, and has a poor hemostatic effect on larger bleeding; microfibrous collagen and collagen sponge are also heterogeneous animal protein products like fibrin glue and gelatin. Risk of immunogenicity and viral transmission; oxidized cellulose is loose and quickly absorbed by the body, but may cause rebleeding, and the highly acidic environment it produces can cause nerve damage through a diffuse chemical mechanism; chitosan, carboxylate Methyl chitosan, alginic acid, modified starch, etc. are natural polysaccharides, which have problems such as poor batch stability and difficult quality control in production

Method used

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  • Gel polypeptide capable of being used for preparing hemostatic material
  • Gel polypeptide capable of being used for preparing hemostatic material
  • Gel polypeptide capable of being used for preparing hemostatic material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Design and synthesis of polypeptide

[0022] The applicant has designed and screened ion-complementary short peptides, so that the screened peptides have the driving force and guiding effect of self-assembly. The driving force of self-assembly refers to the synergistic effect of non-covalent bond forces between molecules, which provides energy backing for the self-assembly of peptide molecules; while the guiding effect of self-assembly refers to the geometric complementarity of the spatial structure of peptide molecules , only when the spatial structure of peptide molecules is complementary, can they be stacked and rearranged in spatial size and direction.

[0023] In order to realize the self-assembly effect of the designed polypeptide combined with the blood, the applicant designed the self-assembling peptide to contain acidic amino acid residues and basic amino acid residues as polar amino acids, and the insertion position and quantity of non-polar amin...

Embodiment 2

[0030] Example 2: Determination of coagulation activity

[0031] In order to evaluate the in vitro coagulation performance of the designed polypeptide, the activated partial thromboplastin time (APTT) and prothrombin time (PT) were used for analytical tests: activated partial thromboplastin time (APTT) and prothrombin time (PT) ) was determined using a commercial kit.

[0032] Blood sample preparation:

[0033] First, SD rats were anesthetized with pentobarbital sodium solution (0.3 μl / 100mg), blood was drawn from the rat femoral artery, and then it was anticoagulated by 3.8% sodium citrate anticoagulant, at 37 ° C, Platelet-poor plasma was obtained by centrifugation at 3000r / min.

[0034] Preparation of peptide solution:

[0035] Precisely weighed short peptide freeze-dried powder was added to ultrapure water to prepare a mother solution with a concentration of 500mg / ml, sealed and stored in a refrigerator at 4°C in the dark for later use, and diluted to a corresponding co...

Embodiment 3

[0043] Carry out atomic force microscope (AFM) detection to the polypeptide whose amino acid sequence is SEQ ID NO:4:

[0044] Place 20 μl of the peptide solution on the newly dissociated mica sheet, let it stand for 15 s, and then wash 3 times along the edge of the droplet with 100 μl of ultrapure water. Stand at room temperature at 30°C, and observe with AFM after the mica sheet dries naturally. The AFM parameters are set to tapping mode, the scanning area is 2μm×2μm, and the scanning frequency is 1.0Hz; the scanning probe (Model: Arrow UHFAuD) parameters: the elastic coefficient (k) is 6 (1.5~21.0) N / m, and the material is Si , uncoated, with a tip radius of (10±2) nm.

[0045] Carry out transmission electron microscopy (TEM) detection to the polypeptide whose amino acid sequence is SEQ ID NO:4:

[0046] Drop 30 μl of peptide solution on the parafilm, then place a 300-mesh carbon-coated copper grid on the sample for about 30 seconds, take out the copper grid with clean tw...

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Abstract

The invention provides gel polypeptide capable of being used for preparing a hemostatic material. The amino acid sequences of the polypeptide are SEQ ID NO: 1 to 4. The polypeptide provided by the invention has good blood coagulation effect and forms gel when being in contact with blood so as to effectively stop bleeding. The polypeptide has high biodegradability and biocompatibility, and can be used for preparing hemostatic products with different forms.

Description

technical field [0001] The invention provides a gel polypeptide used for preparing hemostatic materials. The provided polypeptide can effectively stop bleeding and belongs to the technical field of medical material preparation. Background technique [0002] In daily life, due to trauma, surgery and other conditions that lead to massive bleeding, if the bleeding is not stopped in time, it may cause infection and aggravate trauma. Therefore, controlling bleeding is an important step in first aid and trauma treatment. Appropriate hemostatic materials can significantly shorten the operation time and are essential for trauma or postoperative recovery. When choosing a hemostatic material, comprehensive consideration should be given to various aspects, including the bleeding site, the shape and size of the operation cavity, the packing requirements of different bleeding situations, the hemostatic mechanism of different hemostatic materials, the compatibility with the body, the pati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61K9/08A61P7/04A61L15/44A61L15/32
Inventor 陈强姜永梅刘姣张凌云姜艳
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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