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Double column production method of gamma-aminobutyric acid by immobilized cell and D101 resin

A D101, aminobutyric acid technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of high cost of biosynthesis of GABA

Active Publication Date: 2017-10-24
LINGNAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0009] The object of the present invention is to provide a kind of by D101 macroporous adsorption resin column and immobilized Enterococcus faecium cell column coupling to form the cyclic compound conversion reaction system, through the promoting effect of D101 macroporous adsorption resin to the activity of Enterococcus faecium GAD, finally Realize the purpose of increasing GABA production and solve the problem of high cost of biosynthetic GABA

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  • Double column production method of gamma-aminobutyric acid by immobilized cell and D101 resin

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Embodiment 1

[0045] A kind of method of producing gamma-aminobutyric acid with immobilized cell and D101 resin double-column type of the present invention mainly comprises the following steps:

[0046] ①Refer to the cell immobilization method of Jiao Yang, Wang Jianmin and Yang Shengyuan in the abstract (Journal of Nuclear Agriculture, 2009, 23 (6): 1026-1031), and prepare immobilized Enterococcus faecium GDMCC 60203 by calcium alginate cell embedding method Cells, and then the immobilized Enterococcus faecium GDMCC 60203 cells were loaded into a column with a 0.2 mol / L acetic acid-sodium acetate buffer solution of pH 4.2 according to a conventional method to prepare an immobilized cell reaction column;

[0047] ② Pretreat the D101 macroporous adsorption resin with 95% ethanol and distilled water according to the instructions of the resin, and then prepare the auxiliary reaction column with 0.2 mol / L acetic acid-sodium acetate buffer solution of pH 4.2 according to the conventional method; ...

Embodiment 2

[0057] A kind of method of producing gamma-aminobutyric acid with immobilized cell and D101 resin double-column type of the present invention mainly comprises the following steps:

[0058] ① Referring to the cell immobilization method of Shen Yu et al. in the abstract (Fine Chemical Industry, 2008, 25(5): 459-462), the immobilized Enterococcus faecium GDMCC 60203 cells were prepared by embedding with carrageenan and gelatin, and then the pH 4.2 0.2 mol / L acetic acid-sodium acetate buffer solution to prepare the immobilized Enterococcus faecium GDMCC 60203 cells into the column according to the conventional method to prepare the immobilized cell reaction column;

[0059] ② Pretreat the D101 macroporous adsorption resin with 95% ethanol and distilled water according to the instructions of the resin, and then prepare the auxiliary reaction column with 0.2 mol / L acetic acid-sodium acetate buffer solution of pH 4.2 according to the conventional method;

[0060] ③ Combine the immobi...

Embodiment 3

[0069] A kind of method of producing gamma-aminobutyric acid with immobilized cell and D101 resin double-column type of the present invention mainly comprises the following steps:

[0070] ① Referring to Zhao Weirui et al.’s abstract (Journal of Chemical Engineering of Universities, 2015, 29(1): 138-144), firstly, an organic solvent with low dielectric constant (0.68) was used to treat Enterococcus faecium GDMCC 60203 cells were permeabilized, and then referring to the cell immobilization method of Jiao Yang et al. Enterococcus GDMCC 60203 cells, and then use 0.2 mol / L acetic acid-sodium acetate buffer solution with pH 4.2 to pack the immobilized Enterococcus faecium GDMCC 60203 cells into a column according to a conventional method to prepare an immobilized cell reaction column;

[0071] ② Pretreat the D101 macroporous adsorption resin with 95% ethanol and distilled water according to the instructions of the resin, and then prepare the auxiliary reaction column with 0.2 mol / L...

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Abstract

The invention relates to a double column production method of gamma-aminobutyric acid by immobilized cell and D101 resin, which belongs to the technical field of green biological production; according to the double column production method, a D101 macroporous adsorption resin column is taken as an auxiliary reaction column and is coupled mutually with an immobilized enterococcus faecium cell reaction column, and the coupled macroporous adsorption resin column and the immobilized enterococcus faecium cell reaction column are connected with other auxiliary installation or equipment to form a cyclic reaction system, efficient conversion is realized as a reaction substrate is cycled between the D101 macroporous adsorption resin column and the immobilized enterococcus faecium cell reaction column, and productivity of the gamma-aminobutyric acid is improved; compared with a catalytic reaction method only using the immobilized enterococcus faecium cell reaction column, the output of the gamma-propalanine produced by the invention can be increased by 196.32%. Besides, purification of the gamma-aminobutyric acid also can be realized through the adsorption effect of the D101 resin column, a downstream process is simplified, the cost is reduced, and is environmentally friendly.

Description

technical field [0001] The invention relates to a method for producing gamma-aminobutyric acid with immobilized cells and D101 resin double column, belonging to the field of green biological manufacturing. Background technique [0002] γ-Aminobutyric acid (γ-Aminobutyric acid, GABA) is a non-protein amino acid with 4 carbon atoms. It is the main inhibitory neurotransmitter in the mammalian central nervous system. Antioxidant, anti-inflammatory, anti-cancer, blood pressure lowering, sedative and sleep improving effects have become the ingredients of pharmaceuticals and health products that have attracted much attention. GABA is also an important raw material for the production of bioplastic polyamide 4 due to the formation of 2-pyrrolidone by lactamation. [0003] Microbes grow and reproduce quickly, and their production of GABA is not limited by time and space. Therefore, the production of GABA by microorganism GAD (Glutamate decarboxylase, GAD, EC4.1.1.15) has attracted mu...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12R1/46
CPCC12P13/005
Inventor 杨胜远韦锦
Owner LINGNAN NORMAL UNIV
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