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A kind of preparation method of alginic acid-dopamine/nano hydroxyapatite composite scaffold

A nano-hydroxyapatite and hydroxyapatite technology, which is applied in the fields of polymer materials and biological materials, can solve the problems that the compatibility of human body is not very ideal, achieve suitable porosity, promote tissue regeneration, and achieve good dispersion. sexual effect

Active Publication Date: 2020-05-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a scaffold material for bone repair, its human adaptability and compatibility with natural bone are not ideal

Method used

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  • A kind of preparation method of alginic acid-dopamine/nano hydroxyapatite composite scaffold
  • A kind of preparation method of alginic acid-dopamine/nano hydroxyapatite composite scaffold
  • A kind of preparation method of alginic acid-dopamine/nano hydroxyapatite composite scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1. Synthesis of quaternized chitosan (QCS)

[0016] 4g of chitosan (CS) was dispersed in 100mL of KOH-LiOH.H2O-urea-H2O mixed solution with a mass ratio of 7:8:8:77 to obtain the CS dispersion, which was placed in a refrigerator at -20°C overnight. Then thaw at 5°C and stir until a transparent CS solution is obtained. Then 62.3 mL of (3-chloro-2-hydroxypropyl) trimethylammonium chloride solution (CHPTAC) was added dropwise to the CS solution, and the reaction was stirred at 30° C. for 24 h. After the reaction, the solution was neutralized with hydrochloric acid, and lyophilized after being dialyzed in deionized water for a week. The white flocculent sample obtained was quaternized chitosan (QCS) with a quaternization degree of 96.8%.

[0017] It should be noted that by changing the addition amount of CHPTAC in Example 1 to 7.78, 15.6, and 31.1 mL, quaternized chitosan (QCS) with quaternization degrees of 17.7, 23.3, and 47.2% can be obtained, respectively.

Embodiment 2

[0018] Example 2: Synthesis of quaternized chitosan / hydroxyapatite (QCHA)

[0019] Dissolve a certain amount of QCS in 0.3M Na 2 HPO 4 In the solution, stir at room temperature for 1h; then heat to 90℃, under the condition of mechanical stirring, add a certain amount of 0.5M CaCl with the atomic ratio of Ca / P of 1.67 2 The solution was added to the above QCS solution at 2 mL / min. In this process, 1M NaOH was used to adjust the pH of the reaction solution to about 10. After 2 hours of reaction, a white precipitate was obtained by centrifugation, and the precipitate was washed with deionized water until the pH value was close to neutral, and then lyophilized to obtain QCHA. The theoretical ratio of quaternized chitosan to hydroxyapatite is 3:7, and the corresponding QCHA label is QCHA-30.

[0020] It should be noted that by changing the theoretical ratio of quaternized chitosan to hydroxyapatite in Example 2 to 1:9 and 2:8, QCHA-10 and QCHA-20 can be obtained respectively.

Embodiment 3

[0021] Example 3. Preparation of alginic acid-dopamine

[0022] Weigh 1g of alginic acid (Alg), put it into a three-necked flask, add 100mL PBS buffer (50mM, pH=5.5.), stir to dissolve at room temperature, when it is completely dissolved, add 1.94g 1-ethyl-(3- Dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 2.32g N-hydroxysuccinimide (NHS), n(COOH) / n(EDC) / n(NHS)=1 / 2 / 4, after activation at room temperature for 30 minutes, add 4.79g of dopamine hydrochloride (DA), repeatedly vacuum and pass nitrogen three times to remove air and prevent DA oxidation, react at room temperature under a nitrogen atmosphere for 24 hours, stop the reaction and dialyze in deionized water After removing EDC, NHS and unreacted monomers, until there is no dopamine ultraviolet absorption peak in the dialysate, freeze-drying to obtain a white cotton-like bio-modified macromolecule Alg-DA with a DA substitution degree of 52.6%.

[0023] It should be noted that by changing the amount of DA added in Ex...

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Abstract

The invention provides a preparation method for an alginic acid-dopamine / nano-hydroxyapatite composite bracket. The method comprises the following steps: utilizing (3-chlorine-2-hydroxypropyl) trimethyl ammonium chloride (CHPTAC) to perform quaternization modification on chitosan (CS), thereby acquiring quaternized chitosan (QCS); utilizing QCS as an organic matter to in-situ compound hydroxyapatite, thereby preparing the quaternized chitosan / hydroxyapatite (QCHA) with excellent dispersity; compounding with the dopamine modified alginic acid (Alg-DA) prepared in advance under the effect of electrostatic interaction, thereby acquiring an alginic acid-dopamine / nano-hydroxyapatite (Alg-DA / QCHA) composite bracket material. The structure and performance of the Alg-DA / QCHA composite bracket are regulated in the manner of changing the ratio of Alg-DA to QCHA; the calcium chloride is utilized to crosslink the composite bracket, so that the porosity of the bracket can be adjusted; the Alg-DA / QCHA composite bracket material prepared according to the invention has excellent mechanical property and mineralizing property, suitable degradation period, excellent cytocompatibility and cell adhesion property and wide application prospect in the field of biomedical engineering.

Description

Technical field [0001] The invention relates to a preparation method of an alginic acid-dopamine / nano hydroxyapatite composite scaffold, which belongs to the technical field of polymer materials and biological materials. [0002] technical background [0003] In recent years, with the increase in clinical demand for bone repair materials, bone repair materials have become one of the research hotspots in the field of biomedicine. Human natural bone is an inorganic-organic composite composed of apatite and polymer collagen fibers. The composition of single-component biomedical materials such as metals, ceramics, and polymers that have been widely used in clinical medicine for a long time is different from that. As a bone repair scaffold material, its human body adaptability and compatibility with natural bone are not very ideal. In order to overcome the limitations of single-component bone repair scaffold materials, bone repair composite materials are prepared by combining metal, ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/56A61L27/52A61L27/50
CPCA61L27/46A61L27/50A61L27/52A61L27/56A61L2430/02C08L5/04C08L5/08
Inventor 施冬健沈佳丽石畅李小杰陈明清
Owner JIANGNAN UNIV
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