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Preparation method of tissue engineering acellular vascular scaffold

A vascular stent and tissue engineering technology, which is applied in the field of preparation of tissue engineered decellularized vascular stents, can solve problems such as insufficient mechanical properties, achieve the effects of improving mechanical properties, reducing immune reactions, and improving vascular mechanical properties

Inactive Publication Date: 2017-05-31
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, when these allogeneic or xenogeneic decellularized scaffolds are applied to small-caliber (inner diameter less than 6 mm) vascular grafts, there are problems such as insufficient mechanical properties in the environment of small-caliber and high-tension vessels, which may lead to serious aneurysms.

Method used

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  • Preparation method of tissue engineering acellular vascular scaffold
  • Preparation method of tissue engineering acellular vascular scaffold

Examples

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Embodiment 1

[0019] A preparation method of tissue engineering decellularized vascular scaffold, adopting melt spinning technology to prepare with PCL as raw material, comprising the following steps:

[0020] 1) Add 20 grams of PCL with a molecular weight of 8000 into the barrel of the melt spinning machine, raise the temperature of the barrel to 210°C and keep it for 1h, adjust the distance between the needle of the barrel and the medical silicone tube with an outer diameter of 2mm to 1cm, set The propulsive flow rate of the fixed barrel is 0.5mL / h, the speed of the receiving silicone tube is 300rpm, the horizontal moving speed is 10mm / s, the moving distance is 10cm, and the spinning time is 12min. Mesh vascular stent;

[0021] 2) Cut the above-mentioned PCL ordered mesh vascular stent with silicone tubes into 2cm sections, sterilize with ethylene oxide, anesthetize the rats, shave the sides of the back to grow a 6cm long, 2cm wide rectangular surface, and use Cut a 1 cm long incision wi...

Embodiment 2

[0025] A preparation method of tissue engineering decellularized vascular scaffold, adopting wet spinning technology to prepare PLCL as raw material, comprising the following steps:

[0026] 1) Dissolve 1 gram of PLCL into 10 mL of hexafluoroisopropanol, stir at room temperature until completely dissolved, connect a medical silicone tube with a diameter of 2 mm to the rotating motor, draw the PLCL solution into the syringe, and put the needle of the syringe into the ethanol coagulation bath The middle distance is 1cm from the silicone tube. Set the solution flow rate to 2ml / h, receive the silica gel tube at a speed of 500rpm, and spin the tube for 40 minutes to obtain an orderly network vascular stent with spirally wound fibers outside the silicone tube. After the preparation is completed, the vascular stent is vacuum-dried for later use;

[0027] 2) The above-obtained ordered mesh vascular stent with an inner diameter of 2mm was cut and grown into a vascular stent of 3cm, ste...

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Abstract

The invention discloses a preparation method of a tissue engineering acellular vascular scaffold. Enhanced tissue engineering blood vessels without immunogenicity can be obtained by embedding micron high-molecular polymer scaffold into subcutaneous part or abdominal cavity of a host and performing acellular treatment on the blood vessel scaffold wrapped by utilizing a host immune protection mechanism. The preparation method has the advantages that deficient mechanical performance of the tissue engineering acellular blood vessel can be solved, and the mechanical performance of the original tissue engineering acellular vascular scaffold can be fully improved through a multilayer cross-linked mesh type high-molecular scaffold; the problem of immunogenicity of the tissue engineering blood vessel in allotransplantation can be solved, the scaffold blood vessel formed by migrating cells caused by immunologic mechanism is established in a host animal body, the transplanted immunoreaction can be reduced through acellular treatment, extracellular matrix is fully utilized to provide a good environment for cell regeneration; and different acellular vascular scaffolds can be prepared through designing scaffolds of different shapes and sizes of by utilizing the method, so as to be applied under different blood vessel transplanting conditions.

Description

technical field [0001] The invention relates to a preparation method of tissue engineering decellularized vascular scaffold. Background technique [0002] Cardiovascular disease is the number one killer of human health, and the first choice for more than half of patients is vascular transplantation, but it is difficult for some patients to implement it due to the limitation of their own blood vessel sources. Allogeneic or xenograft blood vessels have been used as a substitute for autologous blood vessels to repair diseased or missing blood vessels, but the potential risk of disease transmission, acute immune rejection and limited donor sources limit their application in transplantation. The development of tissue engineering technology provides a new solution for the repair and reconstruction of human vascular defects. At present, a variety of synthetic and degradable materials have been developed for the construction of tissue engineering scaffolds, which have been applied c...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/36A61L27/50A61L27/58
CPCA61L27/18A61L27/3633A61L27/3687A61L27/50A61L27/507A61L27/58A61L2430/40
Inventor 王恺孔德领程曲汉朱美峰张秋影傅迪宏
Owner NANKAI UNIV
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