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Preparation method of nano emulsion for improving bioavailability of xanthophyll

A technology of nanoemulsion and utilization, which is applied in the direction of emulsion delivery, medical preparation and application of non-active ingredients, which can solve the problems of low bioavailability and poor stability, and achieve the expansion of application range, good stability and efficacy Improved effect

Inactive Publication Date: 2017-05-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the technical deficiencies such as poor stability and low bioavailability of existing lutein emulsions, and to prepare CFG with high stability and bioavailability by extracting CFG with high emulsifying and antioxidant activity. Containing lutein nanoemulsion, its lutein embedding rate reaches 86%, and the bioavailability of lutein in the emulsion increases to 55%, which significantly improves the efficacy of lutein in functional health food

Method used

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  • Preparation method of nano emulsion for improving bioavailability of xanthophyll
  • Preparation method of nano emulsion for improving bioavailability of xanthophyll

Examples

Experimental program
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Effect test

Embodiment 1

[0052] a) Weigh the corn bran sample, add n-hexane containing 0.01% (w / w) di-tert-butyl-p-cresol according to the ratio of material to liquid 1:10 (g / mL), stir slowly for 2h, filter and collect the filter residue, then Washed twice with absolute ethanol;

[0053] b) In the above-mentioned washed sample, add deionized water according to the ratio of material to liquid 1:10 (g / mL), take a boiling water bath for 10 min, adjust the pH of the solution to 6 with NaOH solution (20%, w / w), and the temperature of the water bath Adjust to 95°C, add α-amylase, the amount of enzyme added is 0.3% of the dry weight of the sample, enzymatically hydrolyze for 2h, collect the precipitate by centrifugation, and then dry it at 60°C for 24h for use;

[0054] c) Weighing the dried corn bran treated in step b), adding deionized water at a ratio of 1:10 (g / mL) to the material to liquid, and adding 1.5% (w / w) of corn bran dry mass at the same time NaOH and 1.5% (w / w) Ca(OH) 2 , mechanically stirred...

Embodiment 2

[0063] a) Weigh the corn bran sample, add n-hexane containing 0.01% (w / w) di-tert-butyl-p-cresol according to the ratio of material to liquid 1:10 (g / mL), stir slowly for 2h, filter and collect the filter residue, then Washed twice with absolute ethanol;

[0064]b) To the above washed sample, add deionized water according to the ratio of material to liquid 1:10 (g / mL), boil the water bath for 10min, adjust the pH of the solution to 6.5 with NaOH solution (20%, w / w), and the temperature of the water bath Adjust the temperature to 90°C, add α-amylase, the amount of enzyme added is 0.4% of the dry basis weight of the sample, carry out enzymatic hydrolysis for 2h, collect the precipitate by centrifugation, and then dry it at 60°C for 24h for use;

[0065] c) Weighing the dry corn bran treated in step b), adding deionized water at a ratio of 1:10 (g / mL) to the material to liquid, and adding 2% (w / w) of the dry weight of the corn bran at the same time. NaOH and 2% (w / w) Ca(OH) 2 ,...

Embodiment 3

[0073] a) Weigh the corn bran sample, add n-hexane containing 0.01% (w / w) di-tert-butyl-p-cresol according to the ratio of material to liquid 1:10 (g / mL), stir slowly for 2h, filter and collect the filter residue, then Washed twice with absolute ethanol;

[0074] b) In the above-mentioned washed sample, add deionized water according to the ratio of material to liquid 1:10 (g / mL), take a boiling water bath for 10 minutes, adjust the pH of the solution to 7 with 20% (w / w) NaOH solution, and the temperature of the water bath Adjust the temperature to 85°C, add α-amylase, the amount of enzyme added is 0.5% of the dry basis weight of the sample, carry out enzymatic hydrolysis for 2h, collect the precipitate by centrifugation, and then dry it at 60°C for 24h for use;

[0075] c) Weigh the dry corn bran treated in step b), add deionized water at a ratio of 1:10 (g / mL) to the liquid, and add 2.5% (w / w) of the dry corn bran mass at the same time. NaOH and 2.5% (w / w) Ca(OH) 2 , mechan...

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Abstract

The invention discloses a preparation method of a nano emulsion for improving the bioavailability of xanthophyll. The method comprises the steps: firstly, preparing a corn fiber gum CFG, then dissolving the corn fiber gum CFG, citric acid and sodium benzoate in deionized water, and stirring slowly for 2-3 h, to obtain an aqueous phase; then adding xanthophyll to corn oil, and carrying out ultrasonic treatment to make the xanthophyll completely dissolved, to obtain an oil phase; mixing the oil phase and the aqueous phase, adding deionized water to quantify to 100%, homogenizing by a high-speed shear homogenizer, to prepare a crude emulsion, and carrying out high-pressure homogenization, to prepare the stable xanthophyll nano emulsion. The nano emulsion prepared by the method has the particle size in the range of 100-200 nm, has good storage stability, and has the xanthophyll embedding rate reaching 86%; the bioavailability of the xanthophyll in the emulsion is increased from 10% to 55%, and the efficacy of the xanthophyll in functional health food can be significantly improved.

Description

technical field [0001] The invention relates to the field of functional health food, in particular to a method for extracting natural plant polysaccharide corn fiber gum with antioxidant activity, and a method for preparing a nano-emulsion used as an emulsifier to improve the bioavailability of lutein. Background technique [0002] Carotenoids in plants are forty-carbon isoprenoid polyene chain compounds containing 9 to 15 conjugated double bonds. Lutein is an important member of the carotenoid family, and its content can be as high as 50% of the total carotenoid. Lutein participates in the assembly of photosystem pigment-protein complexes, and has the functions of absorbing, transmitting light energy, dissipating excess excitation energy and anti-oxidation. Natural lutein extracted from plants has the functions of delaying lung aging, protecting retina, reducing cataract and night blindness, and reducing the risk of breast cancer and lung cancer. Due to the existence of n...

Claims

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Application Information

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IPC IPC(8): A23L29/10A23L33/10A61K9/107A61K31/047A61K47/36A61P11/00A61P27/02A61P27/12A61P35/00C08B37/14
CPCA61K9/1075A23V2002/00A61K31/047A61K47/36C08B37/0003A23V2200/222A23V2250/502
Inventor 黄强冯慧敏李超扶雄
Owner SOUTH CHINA UNIV OF TECH
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