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Application of mogrol to preparation of antiviral drugs

Inactive Publication Date: 2016-11-09
CHENGDU BIOPURIFY LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] At present, there is no clinical report on the use of mogrosanol for antivirals, and there is no report on the combination of mogrosanol and existing antiviral drugs for antiviral treatment or / and prevention

Method used

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  • Application of mogrol to preparation of antiviral drugs
  • Application of mogrol to preparation of antiviral drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Effect of Mogrosanol on the expression of TLRs receptors in suckling mice infected with rotavirus

[0055] 1. Experimental materials

[0056] LLC-MK2 cells (virus culture cells); simian rotavirus SA11 strain; natural delivery of suckling mice (meet clean-grade requirements, average body weight 1.88g, breastfeeding after birth, laboratory environment: SPF); 1640 medium; fetal cow Serum; trypsin; double antibody; rotavirus detection kit; Trizol total RNA extraction kit; SYBR Green qPCRSuperMix; cell growth medium (under strict sterile conditions, take 90ml of 1640 culture medium and add 10ml of fresh fetal bovine serum, 4 ℃ storage); virus maintenance solution (trypsin was added to 1640 medium, the concentration was 1 μg / ml); water-proof constant temperature incubator; ultra-low temperature refrigerator; optical microscope; electronic balance; liquid nitrogen tank; low-temperature centrifuge; fluorescent PCR instrument . All target gene sequences used were syn...

Embodiment 2

[0070] Example 2 Effect of Mogrosanol on Hepatitis C Virus Activity

[0071] 1. Experimental materials and instruments

[0072] Huh7.5.1 cells; plasmid pJFH1 containing the complete genome sequence of HCV2a type JFH1 virus strain; virus JFH1-5AGFP with reporter gene; DMEM medium (purchased from GIBCO); MTT detection reagent (purchased from biomol); Endonuclease (purchased from NEB); Zeiss advanced inverted microscope Axio observer A1 (Carl Zeiss); PerkinElmer multi-function detector; mogrosin (self-made, purity 98%).

[0073] Experimental method and results

[0074] 2.1 Construction of plasmid pJFH1-5AGFP and preparation of virus JFH1-5AGFP

[0075] Plasmid transformation was carried out on pJFH1, and a restriction site Xho (nt7523-nt7528, aa419-aa420) at the C-terminus of the NS5A coding region was selected and inserted into the EGFP gene (amplified from the PEGFP-N1 plasmid). After the plasmid was successfully constructed, it was sequenced and identified. Using the plasmi...

Embodiment 3

[0091] Example 3 Anti-HIV activity experiment of mogrosvenol

[0092] In this experiment, the cytopathic effect method (CPE) and RNA reverse transcription fluorescent quantitative PCR method were used to evaluate the anti-HIV effect of Mogrosanol in vitro.

[0093] 1 Materials and reagents

[0094]CEMxl74 cells and HIV-1 virus (both from the Aarond Diamond AIDS Research Center in the United States, donated by the Institute of Experimental Animals, Chinese Academy of Medical Sciences); DMSO (imported by the Academy of Military Medical Sciences); RPMI Medium 1640basic (1×), F etalBovine Serum (Shanghai Lifei Biotechnology Co., Ltd.); Zidovudine (AZT, 3'-Azido-3'-deoxythymidine) was purchased from Sigma. The mRNA quantitative PCR primers of each gene were synthesized by Shanghai Handsome Biotechnology Co., Ltd. Monk Fruit Alcohol (self-made, purity greater than 98%).

[0095] 2. Test method

[0096] 2.1 Cell culture

[0097] The CEMx174 cell line was inoculated in RPM11640 m...

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Abstract

The invention provides new medical application of mogrol and particularly relates to medical application of the mogrol and the related compounds of the mogrol to the treatment or / and prevention of viral infection. The invention further provides application of the mogrol and the related compounds of the mogrol to the preparation of drugs for treating or / and preventing the viral infection and particularly relates to application of the mogrol and the related compounds of the mogrol to the resisting of hepatitis C viruses and HIV viruses. Experiments prove that the mogrol can evidently activates the expression of body TLRs receptors so as to activate the innate immunity system of a human body to bring an antiviral effect into play. Experiment researches show that the mogrol has a high inhibiting and eliminating effect on the hepatitis C viruses and HIV viruses and is a potential drug for resisting the hepatitis C viruses and HIV viruses. The mogrol is prepared from natural plants which are wide in distribution and rich in resource, so that the mogrol is low in industrial preparation cost and capable of being favorably popularized and applied.

Description

technical field [0001] The invention belongs to the field of natural medicine preparation, and relates to a natural compound that can be used to prevent or / and treat viral infection, in particular, it relates to the antiviral medical use of mogrosvenol and its related compounds and their application in the preparation of antiviral medicines. Background technique [0002] Viruses can be simply divided into non-enveloped viruses and enveloped viruses according to whether their shells are wrapped with lipid-rich membranes. Non-enveloped viruses mainly enter infected cells through pinocytosis mediated by clathrin; enveloped viruses mainly enter the infected cells through the fusion process of the viral envelope and the host cell membrane. According to the conformational changes of envelope surface glycoproteins during virus invasion, enveloped viruses can be divided into three categories: Orthomyxoviridae, Retroviridae, Filoviridae, Paramyxoviridae and Coronaviridae are It is c...

Claims

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Application Information

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IPC IPC(8): A61K31/575A61K45/06A61P31/12A61P31/14A61P31/16A61P31/20A61P31/22A61P31/18
CPCA61K31/575A61K45/06A61K2300/00A61K31/12A61K31/14A61K31/16A61K31/20A61K31/22Y02A50/30
Inventor 罗明锋谢海峰胡云岭谢期林张朝凤郭建龙
Owner CHENGDU BIOPURIFY LTD
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