Three-dimensional porous sponge scaffold with meniscus matrix source and preparation method and application

Abstract

The invention discloses a three-dimensional porous sponge scaffold with a meniscus matrix source and a preparation method and an application. The method comprises the following steps: with meniscus as a raw material, removing cells through mechanical crushing treatment, acetic acid enzyme digestion and a differential centrifugation method to obtain meniscus extracellular matrix slurry; building a three-dimensional porous scaffold through freeze-drying; and carrying out crosslinked reinforcement on the scaffold through ultraviolet and chemical methods. The prepared three-dimensional porous sponge scaffold with the meniscus matrix source has a controllable microstructure, appropriate degradation rate, good biocompatibility and certain biomechanical strength, is beneficial to cell adhesion, uniform distribution of seed cells to enter into the scaffold and proliferation migration growth, is free of immunological rejection when implanted in vivo, can be widely applied to the field of meniscus and cartilage tissue engineering, and has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of medical tissue engineering, and relates to a scaffold for tissue engineering, in particular to a method for preparing a scaffold for tissue engineering that promotes meniscal fibrocartilage and articular cartilage regeneration and a scaffold product prepared by the method. Background technique [0002] The meniscus is a kind of fibrocartilage tissue located between the femur and the tibia of the knee joint. Once the meniscus is damaged, especially in the inner region, due to its very limited self-repair ability, the treatment method is relatively monotonous, and a total or partial meniscus resection has to be performed. However, after meniscectomy, the knee meniscus completely or partially loses the biomechanical function of the normal meniscus, which will cause articular cartilage degeneration in the long run and lead to severe osteoarthritis. Allogeneic meniscus transplantation can replace the missing ...

AI Technical Summary

Problems solved by technology

The disadvantages of artificial synthetic materials are: hydrophobicity, which is not conducive to cell adhesion, the product is acidic when degraded, and it is easy to cause inflammation in the body, etc.

However, the collagen fibers and components of the material are quite different from those of the natural meniscus, and the degradation of the material will affect the microenvironment of the damaged repair site, which is not conducive to tissue reconstruction and regeneration

Patent 201210224724.5 provides a biomaterial for meniscus tear repair and its preparation method. The biomaterial is composed of acellular matrix membrane, a double-layer porous collagen structure with cytokines and fibrochondrocytes, and a fibrochondrocyte membrane on the surface Composition, the microenvironment of meniscus regeneration constructed of biological materials is also difficult to fully meet the special requirements of meniscus regeneration

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