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3D stent for constructing in-vitro tumor model and preparation method and application of 3D stent

A construct, 3D technology, applied in the field of biomedical materials, can solve the problems of low cell culture efficiency, long cycle, signal stimulation, etc., and achieve the effect of important clinical application value, moderate degradation rate and moderate expansion rate.

Pending Publication Date: 2020-01-24
THE FIFTH PEOPLES HOSPITAL OF SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] 2. Using cell culture dishes or cell culture plates to culture cells has the disadvantage of the cell microenvironment being too simple. According to literature review, there are complex signal stimuli such as physical, chemical and biological signals in the microenvironment of colorectal cancer cells in vivo, while cell culture plates Or cell culture dishes are difficult to fully simulate the above physical, chemical and biological signals;
[0007] 3. The culture area of ​​2D cell culture plate or petri dish is relatively limited. It takes 2-3 days to culture colon cancer cells for cell passage, which is time-consuming and laborious. The efficiency of cell culture is not high, and passage is an exogenous operation.
[0008] 4. The use of nude mice to construct an in vitro colon cancer model requires a large amount of scientific research funding, an SPF-level animal room (difficult to meet in grass-roots scientific research institutions), and complex ethical review procedures. The success rate of constructing an in vitro colon cancer model is low and the cycle is long
[0010] There is no report about a 3D scaffold for constructing an in vitro tumor model of the present invention and its preparation method and application

Method used

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  • 3D stent for constructing in-vitro tumor model and preparation method and application of 3D stent
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  • 3D stent for constructing in-vitro tumor model and preparation method and application of 3D stent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1 prepares silk fibroin solution

[0077] The preparation method is as follows:

[0078] (1) Take the clean cocoon shell and cut it into 0.5-1cm 2 1000ml of 0.5% sodium carbonate solution is prepared, and heated to boiling in a water bath, 5g of silkworm cocoon shells are immersed in 0.5% sodium carbonate solution, stirred with a stirring rod, and silkworm cocoon Boil the shell 3 times, 1 hour each time;

[0079] (2) Wash the silkworm cocoon with natural water for 3 times, then wash with deionized water for 3 times, then oven at 60°C for 10 hours;

[0080] (3) Dissolve the dried silkworm cocoons in (2) in lithium bromide solution, configure 9M lithium bromide solution, stir and heat with a magnetic stirrer at 60°C, add the dried silk fibroin to fully dissolve the silk fibroin; funnel filter;

[0081] (4) Dialysis: the filtrate was packed into a dialysis bag and dialyzed in a 4°C refrigerator with deionized water for 5 days, and the water was changed every...

Embodiment 2

[0084] Embodiment 2 prepares chitosan solution

[0085] The preparation method is as follows:

[0086] Weigh 1.5g of CS powder, add deionized water to make up to 100ml, and then add 2ml of glacial acetic acid solution to obtain a light yellow CS solution with a concentration of 1.5% and high viscosity.

Embodiment 3

[0087] Embodiment 3 prepares alginate solution

[0088] The preparation method is as follows:

[0089] Weigh 1.5g of sodium alginate, prepare 100ml of solution with distilled water, stir for 1.5h in a constant temperature water bath at 50°C, prepare a sodium alginate solution with a concentration of 1.5%, let stand at 10°C for 24h for degassing, and set aside.

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Abstract

The present invention relates to the technical field of biomedical materials and particularly to a 3D stent for constructing an in-vitro tumor model and a preparation method and an application of the3D stent. The 3D stent mainly comprises the following raw materials in parts by weight: SF, Cs and Alg at a ratio of 1:1:1. The present invention also comprises the application of the 3D stent for constructing the in-vitro tumor model. Advantages are as follows: step 1, by selecting each raw material and the optimal proportion among the raw materials, and preferably selecting the preparation method, the 3D obtained stent is moderate in degradation rate, moderate in expansion rate, high in water absorption and high in porosity; step 2, a 3D in-vitro culture system is used for replacing 2D cellculture for in-vitro cell culture of colon cancer, biological behaviors of the colon cancer cells in vivo are simulated more truly, and a more reliable basic research basis is provided for diagnosis and treatment of the colon cancer; and step 3, the in vitro tumor model constructed by the stent provides simplicity and convenience for basic scientific research institutions and also is close to growth state of in-vivo tumors.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular, a 3D scaffold for constructing an in vitro tumor model, its preparation method and application. Background technique [0002] Cell culture work has been widely used in various fields such as biology, medicine, toxicology, new drug research and development, bioengineering, etc. The technical means of studying and observing the morphological structure and life activities of cells has become a necessary screening and prediction method for life research. experimental means. [0003] At present, the conventional cell culture mode includes two-dimensional cell culture, which is carried out in cell culture plates such as 2, 4, 6, 12, 24, 48, 96 well cell culture plates or cell culture flasks, bioreactors, etc. Adherent monolayer growth in culture medium in a two-dimensional manner. However, multiple studies have found that the gene expression, signal transduction, and morph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C07K14/435C07K1/34
CPCC12N5/0693C07K14/43586C12N2533/50C12N2533/74C12N2533/70C12N2513/00
Inventor 柯重伟陈亮苏献豪韩善亮洪亮
Owner THE FIFTH PEOPLES HOSPITAL OF SHANGHAI
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