Preparation method of nanometer bone tissues scaffold material

A technology of scaffold materials and bone tissue, applied in the field of biomedical materials, can solve the problems of difficult control and limitation of mechanical properties, low activity of bone scaffolds, etc., and achieve the effects of low cost, good adhesion, and simple and easy-to-operate preparation process

Inactive Publication Date: 2018-09-21
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current composite method has disadvantages such as low activity of the bone scaffold and difficult to control the mechanical properties. In addition, the attachment and proliferation of cells on the bone scaffold requires the help of active substances, which limits the preparation method of the bone scaffold.
The above problems limit the application of chitosan and phosphorus-calcium composite scaffolds in the field of bone tissue, which is also the problem that the invention needs to solve

Method used

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  • Preparation method of nanometer bone tissues scaffold material
  • Preparation method of nanometer bone tissues scaffold material
  • Preparation method of nanometer bone tissues scaffold material

Examples

Experimental program
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Embodiment 1

[0029] (1) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 .4H 2 O solution and, 0.2M (NH 4 ) 2 HPO 4 Add the solution into a three-necked flask according to the calcium-phosphorus ratio of 1.65:1, stir and mix evenly, adjust the pH to 11 with 25% ammonia water, and obtain a white powder precipitate. After filtering, wash the white powder with deionized water and dry it in a vacuum After drying at 80°C in an oven, put it into a muffle furnace for calcination at 1125°C for 1 hour, then grind and pass through a 200-mesh sieve to obtain nanopowder.

[0030] (2) Preparation of nano-bone tissue scaffold material: dissolving chitosan in 1wt% acetic acid solution to obtain a chitosan solution with a concentration of 2wt%, adding gelatin and biphasic calcium phosphate nanopowder to the chitosan solution, The mass ratio of chitosan, gelatin, and biphasic calcium phosphate nanopowder is 2:1:1, stirred at room temperature for 24 hours, and then degassed (using...

Embodiment 2

[0032] The preparation process is basically the same as in Example 1, except that chitosan, gelatin, and biphasic calcium phosphate nanopowder are mixed according to the mass ratio of 2:1:2 in step (2), and the nano bone tissue scaffold material obtained at last is denoted as S2 . The SEM figure of the nano-bone tissue scaffold material that embodiment 2 makes is shown in figure 2 .

Embodiment 3

[0034] The preparation process is basically the same as in Example 1, except that in step (2), chitosan, gelatin, and biphasic calcium phosphate nanopowder are mixed according to the mass ratio of 2:1:3, and the final nano bone tissue scaffold material is denoted as S3 . The SEM figure of the nano-bone tissue scaffold material that embodiment 3 makes is shown in image 3 .

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Abstract

The invention relates to a preparation method of a nanometer bone tissues scaffold material, and belongs to the technical field of biomedical materials. A precipitation method is firstly used to prepare a biphasic phosphate calcium nanopowder, then gelatin and the biphasic phosphate calcium nanopowder are added into a chitosan liquid, the mass ratio of the chitosan to the gelatin to the biphasic phosphate calcium nanopowder is (1-2):1:(0.5-4), the mixture is stirred for 20-40 hours at the room temperature and is then degassed, an obtained solution is poured into a 24-pore plate to circularly freeze and unfreeze and is finally dried to obtain the nanometer bone tissues scaffold material. Compared with the prior art, the preparation process is simple and is easy to operate, the nanometer bone tissues scaffold material can be prepared on a large scale, the cost of the raw material is relatively low, no pollution is caused, the degradation rate of the prepared tone skeleton is appropriate,the bioactivity is better, the cell can adhere better and propagate better, and the nanometer bone tissues scaffold material is better applied to bone repair.

Description

technical field [0001] The invention relates to a preparation method of a nanocomposite bone scaffold material, belonging to the technical field of biomedical materials. Background technique [0002] Chitosan is obtained by deacetylation of chitin that exists widely in nature. This natural polymer has excellent properties such as biocompatibility, blood compatibility, safety, and microbial degradability. It can provide support for scaffolds. Biological activity enables cells to attach to the bone scaffold, and is a better bone scaffold material in the field of biomedicine. However, when chitosan is used as a bone scaffold material, it has the disadvantage of insufficient mechanical properties. It needs to be cross-linked to enhance the mechanical properties. The cross-linking agent is usually glutaraldehyde or water-soluble carbodiimide, but the introduction of the cross-linking agent will reduce the strength of the scaffold. cell compatibility. [0003] Bone tissue contai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/12A61L27/20A61L27/54A61L27/50
CPCA61L27/222A61L27/12A61L27/20A61L27/50A61L27/54A61L2300/232A61L2300/412A61L2400/12A61L2430/02C08L5/08
Inventor 聂磊武巧运马爽李培董婧
Owner XINYANG NORMAL UNIVERSITY
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