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Preparation method of composite bone scaffold material

A technology of bone scaffold and hydroxyethyl chitosan, which is applied in the field of biomedical materials, can solve the problems of reducing scaffold cell compatibility and insufficient mechanical properties, and achieve good adhesion, simple and easy preparation process, and good biological activity. Effect

Inactive Publication Date: 2018-09-04
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a bone scaffold material, chitosan has insufficient mechanical properties and needs to be cross-linked. The cross-linking agent is usually glutaraldehyde or water-soluble carbodiimide, but the introduction of the cross-linking agent will reduce the cytocompatibility of the scaffold.

Method used

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  • Preparation method of composite bone scaffold material
  • Preparation method of composite bone scaffold material
  • Preparation method of composite bone scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of a composite bone scaffold material, comprising the steps of:

[0028] (1) prepare hydroxyethyl chitosan: get 10g chitosan and join in the 50wt% sodium hydroxide solution of 16.7g and carry out alkalization, obtain chitosan solution, described alkalization is carried out in-20 ℃ refrigerator, The alkalization time is 24h, after standing at -20°C for 1h, add the solvent isopropanol, stir and heat up to 78°C, then stir and cool to room temperature, then add a mixed solution of 133ml of isopropanol and 18.3ml of 2-chloroethanol , after stirring evenly, use 0.1M hydrochloric acid solution to adjust the pH to 7, then use absolute ethanol to wash 3 times, then use a dialysis bag with a molecular weight cut-off of 10000Da to dialyze for 2 days, and finally freeze-dry to obtain hydroxyethyl chitosan;

[0029] (2) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 .4H 2 O solution and 0.2M (NH 4 ) 2 HPO 4 The solution was added in...

Embodiment 2

[0034] A preparation method of a composite bone scaffold material, comprising the steps of:

[0035] (1) prepare hydroxyethyl chitosan: get 10g chitosan and join in the 50wt% sodium hydroxide solution of 16.7g and carry out alkalization, obtain chitosan solution, described alkalization is carried out in-20 ℃ refrigerator, The alkalization time is 24h, after standing at -20°C for 1h, add the solvent isopropanol, stir and heat up to 78°C, then stir and cool to room temperature, then add a mixed solution of 133ml of isopropanol and 18.3ml of 2-chloroethanol , after stirring evenly, use 0.1M hydrochloric acid solution to adjust the pH to 7, then use absolute ethanol to wash 3 times, then use a dialysis bag with a molecular weight cut-off of 10000Da to dialyze for 2 days, and finally freeze-dry to obtain hydroxyethyl chitosan;

[0036] (2) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 .4H 2 O solution and 0.2M (NH 4 ) 2 HPO 4The solution was added int...

Embodiment 3

[0041] A preparation method of a composite bone scaffold material, comprising the steps of:

[0042] (1) prepare hydroxyethyl chitosan: get 10g chitosan and join in the 50wt% sodium hydroxide solution of 16.7g and carry out alkalization, obtain chitosan solution, described alkalization is carried out in-20 ℃ refrigerator, The alkalization time is 24h, after standing at -20°C for 1h, add the solvent isopropanol, stir and heat up to 78°C, then stir and cool to room temperature, then add a mixed solution of 133ml of isopropanol and 18.3ml of 2-chloroethanol , after stirring evenly, use 0.1M hydrochloric acid solution to adjust the pH to 7, then use absolute ethanol to wash 3 times, then use a dialysis bag with a molecular weight cut-off of 10000Da to dialyze for 2 days, and finally freeze-dry to obtain hydroxyethyl chitosan;

[0043] (2) Preparation of biphasic calcium phosphate nanopowder: 0.4M Ca(NO 3 ) 2 .4H 2 O solution and 0.2M (NH 4 ) 2 HPO 4 The solution was added in...

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Abstract

The invention relates to a preparation method of a composite bone scaffold material. The preparation method comprises the following steps: firstly, preparing hydroxyethyl chitosan; then preparing double-phase phosphorus-calcium nano-powder by adopting a precipitation method; then preparing a polyvinyl alcohol solution; finally, adding the polyvinyl alcohol solution and the double-phase phosphorus-calcium nano-powder into a hydroxyethyl chitosan solution according to the specific mass ratio; after uniformly stirring, carrying out circulating freezing and unfreezing treatment, and freezing and drying to obtain the composite bone scaffold material. Compared with the prior art, the cellular biocompatibility of a bone scaffold is improved through selecting the hydroxyethyl chitosan; the phosphorus-calcium nano-powder is added into the bone scaffold so that the bone scaffold has a proper degradation speed; a circulating freezing and unfreezing method is adopted and is used for carrying out physical crosslinking on a natural polymer; a chemical crosslinking agent does not need to be added; the bone scaffold provided by the invention has better bioactivity and cells can be attached betterand can be proliferated better; the bone scaffold is applicable to the field of bone tissue engineering.

Description

technical field [0001] The invention relates to a preparation method of a composite bone scaffold material, belonging to the technical field of biomedical materials. Background technique [0002] Bone tissue contains a large amount of phosphorus-calcium materials, such as tricalcium phosphate, hydroxyapatite, calcium carbonate, etc., so phosphorus-calcium materials are often used to prepare artificial bone scaffolds. Calcium phosphate-based biomaterials such as hydroxyapatite (HA, Ca 10 (PO 4 ) 6 (OH) 2 ), tricalcium phosphate (β-TCP, Ca 3 (PO 4 ) 2 ) and biphasic calcium phosphate (BCP) can be used for bone scaffold preparation. BCP is a mixture of HA and β-TCP, composed of a certain proportion of inorganic substances tricalcium phosphate and hydroxyapatite, biphasic calcium phosphate can overcome the shortcomings of tricalcium phosphate easy to degrade and hydroxyapatite not easy to degrade, and has good Osteoconductive and osteoinductive. Among various calcium ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/58A61L27/50A61L27/46
CPCA61L27/58A61L27/46A61L27/50A61L2430/02C08L5/08C08L29/04
Inventor 聂磊武巧运侯孟娟孙萌董婧
Owner XINYANG NORMAL UNIVERSITY
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