Preparation and application of biological adhesion enhanced temperature-sensitive chitosan-based postoperative adhesion prevention hydrogel
A sensitive chitosan-based, bioadhesive technology, applied in the fields of medicine and health, can solve the problems of short retention time, insufficient bioadhesion, difficult burden on patients, etc., and achieves difficult displacement or falling off, suitable degradation speed, and convenient injection and use. Effect
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Embodiment 1
[0100] The synthesis of embodiment 1, hydroxybutyl chitosan
[0101] Alkalinization: Weigh 300mg of chitosan with molecular weights of 1000kD, 600kD, and 100kD respectively and place them in a 50mL conical flask, add 50% (w / v) potassium hydroxide aqueous solution, stir, fully swell for 2h, and then place at room temperature Stir and alkalinize for 24h.
[0102] Dispersion in isopropanol / water system: the alkalized chitosan was suction filtered to remove excess lye, and stood at 4°C for 12 hours. Take 10mL of isopropanol and 10mL of deionized water and mix them at a volume ratio of 1:1 to obtain an aqueous solution of isopropanol. Add chitosan after alkalization and standing to a 1:1 aqueous solution of isopropanol, and stir at room temperature for 24 hours until the alkali Chitosan was completely dispersed in isopropanol / water system.
[0103] Grafting reaction: Add 20mL 1,2-epoxybutane dropwise to the isopropanol / water system dispersed with alkalized chitosan, stir while ad...
Embodiment 2
[0106] Embodiment 2, hydroxybutyl chitosan infrared spectrometry
[0107] Weigh an appropriate amount of HBC and grind it with KBr powder at room temperature with a relative humidity -1 scan in range. Infrared spectra of HBC as figure 1 shown. 3400cm -1 The strong absorption peaks superimposed on the left and right -OH and -N-H vibrational absorption peaks are the characteristic peaks of chitosan, and the characteristic peaks of chitosan are retained after the introduction of hydroxybutyl. 2870cm -1 -2980cm -1 Between the three -C-H vibrational absorption peaks in the hydroxybutyl carbon chain and the 1463cm -1 The newly introduced -CH on the hydroxybutyl group near 3 The absorption peak of is the characteristic peak of HBC. Due to the newly introduced hydroxybutyl group, at 1155cm -1 Near-C 6 The characteristic band of -OH becomes weak and almost disappears. The infrared spectra of HBC prepared from chitosan with different molecular weights proved that the hydroxybu...
Embodiment 3
[0108] Embodiment 3, the use concentration of hydroxybutyl chitosan gel is determined
[0109] The determination of the HBC phase change time adopts the "test tube method", that is, take 1mL hydrogel and add it to a 10mL centrifuge tube, put it in a 37°C water bath, and observe whether the phase change occurs every 5s. The standard for the phase change is to place the centrifuge tube upside down. On the desktop, the HBC in the centrifuge tube does not flow down the tube wall within 30s, and the time that the centrifuge tube has been immersed in the water bath when it is taken out of the water bath is the temperature-sensitive gel phase transition time, accurate to 5s.
[0110] Prepare a 1.5%-5% (w / v) HBC aqueous solution, operate according to the aforementioned method, observe the phase change of HBC solutions with different concentrations in a 37°C water bath and record the phase change time, and plot the phase change time with the concentration change curve, such as figure...
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