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Cultivation method for phellinus igniarius strains from solid culture medium to liquid culture medium

A technology of liquid medium and solid medium, which is applied in the field of cultivation of Phellinus solid strains to liquid medium, can solve the problems of high pollution rate, low yellowing rate, slow mycelium germination speed, etc., and achieve low pollution rate , good growth and improved yellowing rate

Inactive Publication Date: 2016-01-13
四川中瑞华航商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to solve the problems of slow mycelium germination speed, high pollution rate, and low yellow yield rate in the cultivation method of the existing Phellinus japonica mother species, and provide a Phellinus solid-state bacterial strain-transformed liquid medium that solves the above-mentioned problems cultivation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The cultivation method of Phellinus solid-state bacterial seed transfer liquid medium, comprises the following steps:

[0042] (1) Obtain the mother species of Phellinus japonica, inoculate the mother species of Phellinus japonica into solid medium and culture in the dark for 8-12 days.

[0043] (1.1) Preparation of Phellinus japonica

[0044] Cut the blocky tissue from the fruit body of Phellinus radix, inoculate the blocky tissue into the culture medium of the test tube, and cultivate it in a constant temperature dark room until yellow fluffy mycelium grows;

[0045] That is, in the sterilized inoculation box, the surface-sterilized wild Phellinus fruiting body is divided into two halves with both hands, and a small square tissue of about 2*2mm is cut out. Use an inoculation needle to transfer the bacterial flesh tissue to the center of the test tube culture medium. Among them, the utensils and the cut tissue must not be in contact with other parts of the Phellinus t...

Embodiment 2

[0064] The difference between this embodiment and embodiment 1 is only that: the composition of solid culture medium and liquid culture medium in this embodiment is different, and concrete composition is as follows:

[0065] The solid medium comprises the following components by weight percentage: 22% mulberry wood chips, 50% oak wood chips, 25% wheat bran, 1.2% brown sugar, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 1.5% gypsum powder; The rate is 60%;

[0066] The liquid medium includes the following components in parts by weight: 4g of corn flour, 4g of wheat bran, 3g of soybean flour, 2g of potassium dihydrogen phosphate, 10.01g of VB, 1g of magnesium sulfate, 20g of brown sugar, and 1000ml of mulberry juice.

[0067] After testing: Phellinus fruiting bodies grow quickly and neatly. After the ear buds grow for 23 days, the fruiting bodies can reach a length of 6.3cm, a width of 4.8cm, and a thickness of 2.5cm. The color is bright yellow to faded, mostly ...

Embodiment 3

[0069] The difference between this embodiment and embodiment 1 is only that: the composition of solid culture medium and liquid culture medium in this embodiment is different, and concrete composition is as follows:

[0070] The solid medium includes the following components by weight percentage: 30% mulberry wood chips, 47.5% oak wood chips, 20% wheat bran, 1% brown sugar, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 1% gypsum powder; The rate is 60%;

[0071] The liquid medium includes the following components in parts by weight: 3g of corn flour, 5g of wheat bran, 3g of soybean flour, 2g of potassium dihydrogen phosphate, 10.01g of VB, 1g of magnesium sulfate, 20g of brown sugar, and 1000ml of mulberry juice.

[0072] After testing: Phellinus fruiting bodies grow quickly and neatly. After the ear buds grow for 25 days, the fruiting bodies can reach a length of 6.3cm, a width of 4.8cm, and a thickness of 2.5cm. The color is bright yellow to faded, mostly semic...

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Abstract

The invention discloses a cultivation method for phellinus igniarius strains from solid culture medium to liquid culture medium, which is mainly used for solving the problems of the existing phelllinus igniarius mother strain cultivation method that the mycelium germination speed is low, the pollution rate is high and the flavones yield is relatively low. The method comprises the following steps: (1) acquiring a phellinus igniarius mother strain, and inoculating the phellinus igniarius mother strain into a solid culture medium to be cultured in a light-proof manner; and (2) inoculating the strain in the solid culture medium into a liquid culture medium, and culturing the strain to obtain phellinus igniarius liquid strain, wherein the solid culture medium comprises the following components by weight percent: 20 to 30 percent of mulberry sawdust, 40 to 50 percent of xylosma sawdust, 20 to 27 percent of wheat bran, 1 to 1.5 percent of brown sugar, 0.2 percent of monopotassium phosphate, 0.1 percent of magnesium sulfate, and 1 to 1.5 percent of gypsum powder; and the water content is 60 to 63 percent. The cultivation method has the advantages that the growth speed can be increased by 30 to 40 percent, the flavones yield is increased by 20 percent, the yield is high, the sporocarp quality is good, the technology is complete, the reusability is realized, the economic benefit is good, and the like.

Description

technical field [0001] The invention relates to a method for cultivating Phellinus cultivars, in particular to a cultivation method for converting Phellinus solid strains into liquid medium. Background technique [0002] Phellinus, Basidiomycotina, Phyllomycetes, Polyporacea, Polyporaceae, Pinfoliobacteria fungi. Its scientific name is Phellinus Linteus, and its Chinese name is Acupunculus clefthoof, commonly known as Sangchen, Phellinus sphaerifera, etc.; it is a medicinal fungus that parasitizes broad-leaved trees such as mulberry trees, and is known as "forest gold". According to the "Properties of Medicine" record: sweet and pungent, non-toxic. "National Compilation of Chinese Herbal Medicine" records that Phellinus has the effects of "strengthening five viscera, softening firmness, detoxification, hemostasis, promoting blood circulation and stomach diarrhea", and is currently internationally recognized as the most effective medicinal fungus in the field of biological a...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05G3/00A01G1/04
Inventor 张世鑫顾青月熊丛菊李华荣
Owner 四川中瑞华航商贸有限公司
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