Composite feed additive
A compound feed and additive technology, applied in animal feed, animal feed, application, etc., can solve the problems of antibiotic resistance enhancement, quality decline, human health impact, etc., and achieve the improvement of feed-to-meat ratio, reduction of diarrhea rate, good preservation activity and The effect of thermal stability
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Embodiment 1
[0040] Example 1: Acquisition of marine Rhodotorula yeast WYN1 and domestication of copper tolerance
[0041] (1) Fresh sea shrimp, starfish, seaweed and other biological samples collected from the Yellow Sea are placed in sterile YEPD medium, and red or pink round colonies are picked for further purification and culture. According to the accumulation of carotenoids in each strain, the strains with high carotenoid production were screened. Among them, the marine rhodotorula WYN1 with higher carotenoid production has oval cells without pseudohyphae; it forms red colonies on the YEPD medium prepared in seawater, with smooth surface and neat edges; it does not produce eggs on the sporulation medium Cystic spores, multilateral budding, no sporulation; no fermentation of sugar, no formation of starch-like compounds, and no inositol synthesis. Based on the above characteristics, it was preliminarily determined to belong to the genus Rhodotorula. Keep ramps in the refrigerator.
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Embodiment 2
[0059] Embodiment 2 A kind of compound feed additive
[0060] Compound feed additive weight fraction composition is as follows:
[0061] 12 parts of antimicrobial peptides, 15 parts of propolis, 20 parts of Ligustrum lucidum extract, 20 parts of red yeast culture, 40 parts of Morchella enzymolysis powder, 8 parts of Scutellaria baicalensis extract, 0.015 parts of tea polyphenols; red yeast is CCTCCNo: M2014592;
[0062] The antimicrobial peptide is a product sold by Shanghai Chupeptide Biotechnology Co., Ltd.;
[0063] Propolis and tea polyphenols are commercially available;
[0064] The preparation method of the Ligustrum lucidum extract is as follows:
[0065] Ligustrum lucidum is crushed, after passing through a 45 mesh sieve, add 5 times the weight of Ligustrum lucidum in anhydrous ethanol for immersion extraction, control the temperature at 40°C, adjust the temperature to 58°C after 3 hours and maintain it for 1.5 hours, then concentrate and dry the extract to obtain e...
Embodiment 3
[0083]A kind of compound feed additive of embodiment 3
[0084] Compound feed additive parts by weight are composed as follows:
[0085] 10 parts of antimicrobial peptides, 10 parts of propolis, 15 parts of Ligustrum lucidum extract, 15 parts of red yeast culture, 50 parts of Morchella enzymolysis powder, 5 parts of Scutellaria baicalensis extract, 0.01 part of tea polyphenols; Rhodotorula is CCTCCNo: M2014592;
[0086] The antimicrobial peptide is a product sold by Shanghai Chupeptide Biotechnology Co., Ltd.;
[0087] Propolis and tea polyphenols are commercially available;
[0088] The preparation method of the Ligustrum lucidum extract is as follows:
[0089] Ligustrum lucidum is crushed, passed through a 40-mesh sieve, added 3 times the weight of Ligustrum lucidum and soaked in absolute ethanol for extraction, the temperature is controlled at 30°C, after 2 hours, the temperature is adjusted to 55°C and maintained for 1 hour, then the extract is concentrated and dried to...
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