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Copper-based human albumin nano-composite with near infrared thermal effect as well as preparation method and application of copper-based human albumin nano-composite

A nanocomposite and blood albumin technology, which is applied in the field of copper-based human serum albumin nanocomposite and its preparation, can solve the problem of weak photothermal heating ability of nanoparticles, limited effect of inhibiting tumors in animals, and low photothermal conversion efficiency, etc. problems, to achieve good tumor targeting, good light stability, and good blood compatibility

Active Publication Date: 2015-12-02
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method solves the problem of poor biocompatibility to a certain extent, the particle size of the prepared nanocomposite is too large, which obviously limits its application in biology.
It has been reported that copper sulfide nanoparticles were synthesized using bovine serum albumin biomineralization (Dalton Trans. It can raise the temperature up to 17.1°C, and the anti-tumor effect in animals is limited, indicating that the nanoparticle has a weak photothermal heating ability, and the photothermal conversion efficiency is not high, and bovine serum albumin may cause immune side effects in the human body, so it cannot be used for clinical use

Method used

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  • Copper-based human albumin nano-composite with near infrared thermal effect as well as preparation method and application of copper-based human albumin nano-composite
  • Copper-based human albumin nano-composite with near infrared thermal effect as well as preparation method and application of copper-based human albumin nano-composite
  • Copper-based human albumin nano-composite with near infrared thermal effect as well as preparation method and application of copper-based human albumin nano-composite

Examples

Experimental program
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Embodiment 1

[0046] Preparation of copper-based protein nanocomposite: Take human serum albumin and configure it into a 15 mg / mL solution for later use. Prepare 50mmol / L copper nitrate solution and 2mol / L NaOH solution to adjust the pH. Take 20mL of human serum albumin solution and place them in two centrifuge tubes, add 4mL of copper acetate solution to them respectively, adjust the pH of the solution to about 8.5 with 2mol / L NaOH solution, stir at room temperature for 2min, and press copper element and sulfur or selenium element 1:2 add 0.1mol / LNa 2 S·9H 2 O or K 2 SeO 3 , placed in a 55°C water bath with magnetic stirring for 2 hours, and after the solution was taken out, it was dialyzed with a molecular weight cut-off dialysis bag of 8000-14000 for 24 hours, with deionized water as the receiving medium, and the water was changed every 4 hours. Then use a 100kD ultrafiltration centrifuge tube for ultrafiltration, ultrafiltration at 4000rpm for more than 10 times, and finally take ou...

Embodiment 2

[0049] During the preparation of the copper-based protein nanocomposite in Example 1, the concentration of human serum albumin was increased to 50, 100 mg / mL, the copper acetate solution was changed to 5, 25 mmol / L, and other steps were the same as in Example 1. With the reduction of the ratio of human serum albumin and copper ions in the reactant, that is, when the input amount of human serum albumin increases, the homogeneous protein nanocomposite with better physical and chemical stability can be prepared.

Embodiment 3

[0051] The pH value of the reaction system during the preparation of the copper-based protein nanocomposite in Example 1 was adjusted to 10, 12.5, and other steps were the same as in Example 1. As the pH of the aqueous phase in the reaction system increases, the absorption strength of the copper-based protein nanocomplex in the near-infrared region of 800-1200 nm can be adjusted.

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Abstract

The invention discloses a copper-based human albumin nano-composite with a near infrared thermal effect as well as a preparation method and an application of the copper-based human albumin nano-composite. Growth of an inorganic copper-based nano-composite is induced by controlling the protein concentration, the concentration of a copper salt, the pH of a reaction solution, the reaction time and the reaction temperature; and the copper-based nano structure of a copper sulphate or copper selenide-coated nano composite employing human albumin as a skeleton is synthesized. The copper-based human albumin nano-composite with the near infrared thermal effect has the characteristics of good dispersity, good light stability, high photothermal conversion efficiency, good in-vivo tumor targeting property and the like, and can be applied to photothermal therapy; and efficient heat removal of tumors is realized.

Description

technical field [0001] The invention relates to a copper-based human serum albumin nanocomposite and its preparation method and application, in particular to a copper-based human serum albumin nanocomposite with near-infrared photothermal effect and its preparation method and application. Background technique [0002] Photothermal therapy (PTT) is a minimally invasive tumor treatment technology that has developed rapidly in recent years. Under the excitation of incident light, the light-absorbing agent targeted to the tumor can use its photothermal conversion effect to generate heat, which can increase the local temperature of the tumor and reach a certain temperature (above 42°C), thereby achieving the effect of killing tumor cells. Through the tumor-targeted delivery of photothermal conversion substances and the selective irradiation of tumor sites, the systemic toxicity of the whole body is greatly reduced. Therefore, photothermal therapy is considered to be a promising t...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/14A61K33/34A61K47/42A61P35/00
Inventor 陈华兵杨涛邓益斌汪勇柯亨特郭正清何慧杨红邹烨璘计双双汪巧莉
Owner SUZHOU UNIV
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