Collagenous fiber non-woven hydroxyapatite biological scaffold material and preparation method thereof

Abstract

The invention relates to a predation technology of an artificial bone repair material in the field of inorganic nonmetallic material and biomedical material, in particular to a collagenous fiber non-woven hydroxyapatite biological scaffold material and a preparation method thereof. The preparation method disclosed by the invention comprises the following steps: taking a collagenous fiber as a matrix, combining a non-woven needle punching technology with a biological biomimetic mineralization method, and uniformly depositing hydroxyapatite with the rod-shaped or wheat ear-shaped structure on the surface of the porous scaffold of the collagenous fiber. The collagenous fiber non-woven hydroxyapatite biological scaffold material prepared by the method has a three-dimensional connected macroporous structure with the pore diameter of 20-500 microns and the porosity of 10-95%, is high in interface bonding force between the collagenous fiber and the hydroxyapatite, achieves the tenacity of the collagenous fiber and the strength of hydroxyapatite simultaneously, and is excellent in mechanical properties. The constituents and the microstructure of the scaffold material are both similar to a natural bone, so as to facilitate differentiation and osteogenesis of mesenchymal stem cells of a human body.

Description

technical field [0001] The invention relates to the preparation technology of artificial bone repair materials in the field of inorganic non-metallic materials and biomedical materials, in particular to a collagen fiber non-woven hydroxyapatite biological scaffold material and a preparation method thereof. Background technique [0002] Large segmental bone defects caused by traffic accidents, trauma, surgical resection, etc. are currently one of the most common and difficult problems in clinical medicine at home and abroad. In recent years, the development of bone tissue engineering research has provided a new idea and method for repairing large bone defects. In bone tissue engineering, bio-scaffold material occupies a very important position. It not only supports and maintains the shape of the original tissue, but also acts as a template, providing a place for cells to lodge, grow, differentiate and proliferate. , thereby guiding the regeneration of damaged tissue and cont...

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Problems solved by technology

Chen Yuyun et al. (patent CN102532585A) used chondroitin sulfate, collagen, and hydroxyapatite as raw materials to prepare a composite scaffold material with a three-dimensional porous structure by foaming and freeze-drying methods, and the porosity of the porous scaffold was controllable, but the The method blends several single materials, the interface binding force is weak, and the mechanical properties are poor; Zhou Changchun et al. (patent CN103341206A) prepared collagen by filling calcium phosphate ceramics with simulated body fluids and using low-temperature aging to simulate biomineralization Three-dimensional porous bone scaffold, the pore diameter of the scaffold material reaches 400 μm, but the preparation method is complicated, and the composite scaffold is a brittle material with poor mechanical strength; Zhang Zhiqi et al. (patent CN102321270) used NaCl as a porogen to prepare a silk fibroin Protein / hydroxyapatite / collagen composite porous scaffold, although the prepared scaffold material has high porosity, moderate water absorption and expansion rate, and simple preparation process, but the research on the mechanical properties of the material has not involved

[0005] For the preparation technology of artificial bone repair materials, in the prior art, there is also a three-dimensional composite dipping and pulling process of chitosan fiber and bioactive glass, but the coating process is uncontrollable, and the preparation process of bioactive glass is limited, resulting in bioactive glass. Glass powder is prone to agglomeration, resulting in uneven coating and weak bonding, which affects the biocompatibility and mechanical properties of composite materials

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