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Application of metabolites of Uvecodiscoides in the control of Phytophthora capsici

A technology for Pseudomonas spp. and Phytophthora capsicum, which is applied in the field of microorganisms and can solve problems such as the types of unsafe microorganism-derived medicines.

Active Publication Date: 2016-02-17
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of endophytic fungus isolated and screened out from the medicinal plant Artemisia annua--Pestalotiopsisuvicola in view of the shortage of unsafe chemical drugs and fewer types of microbial source drugs.

Method used

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  • Application of metabolites of Uvecodiscoides in the control of Phytophthora capsici
  • Application of metabolites of Uvecodiscoides in the control of Phytophthora capsici
  • Application of metabolites of Uvecodiscoides in the control of Phytophthora capsici

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Embodiment 1

[0037] Embodiment 1: (Isolation and screening of Yuweike Discochaete GMH31)

[0038] In August 2011, the present invention collected the healthy leaves of Artemisia annua, a medicinal plant, in the suburbs of Guiyang City, Guizhou Province. After surface disinfection, separation of endophytic fungi, cultivation, fermentation, and activity determination, the active strains of pathogenic bacteria inhibiting plant fungal diseases and Screening of tumor cell lines and other steps, from which Uvecosporum GMH31 was obtained and preserved.

[0039] The specific isolation and cultivation methods are: (1) preparation of Artemisia annua plant tissue: collect the leaf tissue of Artemisia annua, rinse with tap water, remove the superficial microorganisms on the surface of the sample, and then put it on a clean gauze and blow it to dry;

[0040] (2) Indicate disinfection and sterility testing: the dried tissue pieces are cut into appropriate sizes, rinsed with sterilized distilled water, a...

Embodiment 2

[0048] Embodiment 2: (preparation method of Yuwei can be Pseudomonas GMH31 fermentation metabolites)

[0049] Follow these steps:

[0050] (1) Activation culture of strains: move the separated and preserved strains of P. GMH31 from Pseudomonas GMH31 to the potato dextrose agar PDA solid plate, and cultivate them at 28±1°C until the hyphae cover the entire culture dish. With a diameter of 7mm puncher punching to obtain bacteria cake, used for inoculation;

[0051] (2) Liquid fermentation culture: the fermentation medium contains 10g of corn flour, 20g of mannitol, 20g of glucose, 5g of yeast extract, 10g of peptone, and KH per 1000mL. 2 PO 4 0.5g, CaCO 3 15g, MgSO 4 ·7H 2 O0.3g, 100mL of fermentation broth per 300mL Erlenmeyer bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick a bacterium cake with a diameter of 7mm under aseptic conditions, and stand at 28±1°C Place fermentation and culture for 30 days;

[0052] (3) Extraction of fermented liqui...

Embodiment 3

[0055] Embodiment 3: (the inhibitory effect of Yuweike Discotrichum sp. GMH31 fermentation broth metabolites on kiwifruit brown rot fungus and Phytophthora capsici)

[0056] (1) Accurately weigh 0.1 g of the n-butanol fermentation broth extract of Polychaetospermum GMH31, add 10 mL of sterile water, fully dissolve with ultrasonic vibration, and finally prepare a mother liquor with a concentration of 10 g / L;

[0057] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1000mg / L and 500mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C Evenly (the final concentration of metabolites of Uvecodiscoides GMH31 is 100mg / L and 50mg / L), after cooling, put a 7mm diameter cake of kiwifruit brown rot and Phytophthora capsici respectively on each medium plane , the bacteria cake was connected to the center of the petri dish (the d...

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Abstract

The invention relates to the technical field of microorganisms, the artemisia japonica endophytic fungi is isolated from artemisia japonica plant living body which is collected from suburb of Guiyang City Guizhou Province by endophytic fungi separation technology, by microbial taxonomic identification, the artemisia japonica endophytic fungi is named as pestalotiopsis uvicola GMH31, the strain is preserved in China Center For Type Culture Collection (CCTCC), the preservation date is June 30, 2014, and the preservation registration number is CCTCC NO:M2014303. The most striking feature of the Pestalotiopsis uvicola GMH31 is that the fermentation liquor of the strain can produce active metabolites with inhibiting effects on kiwi fruit sclerotinia sclerotiorum, phytophthora capsici and other pathogenic fungi of plant fungal diseases, and the Pestalotiopsis uvicola is an important biological resource in agriculture.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the application of a metabolite of Pestalotiopsisuvicola GMH31 derived from the medicinal plant Artemisia annua in agriculture. Background technique [0002] Plant endophytic fungi (fungal endophyte) refer to fungi in a certain stage or all stages of the life cycle of living and healthy plants in qualified organs. They form a close symbiotic relationship with host plants through interaction, which can promote plant growth and provide plants with the ability to adapt to stress. Some endophytic fungi also have medicinal value. Searching and discovering active compounds from plant endophytic fungi has become another research hotspot at home and abroad. For example, Chinese patent document CN201310194836.5 discloses an endophytic fungus (Metarrhizium) LD421 isolated from gentian, which can be used to prevent and treat gentian leaf blight; The endophytic fungus Fusarium solani T-7 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P3/00A61K36/06A61P35/00C12N1/14C12N1/02C12R1/645
Inventor 钱一鑫康冀川耿坤
Owner GUIZHOU UNIV
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