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Composite artificial bone for enhancing osteogenic activity and preparation method thereof

An osteogenic activity and artificial bone technology, applied in the field of biomedical materials, can solve the problems of limited source of autologous bone transplantation, large immune rejection, and potential safety hazards, so as to improve biological activity and biocompatibility, and promote growth Bone activity, the effect of improving mechanical properties

Active Publication Date: 2014-07-16
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used autologous bone and allogeneic bone grafts have deficiencies to varying degrees. For example, although autologous bone grafts have no immune rejection and have good osteoinductivity, the sources of autologous bone grafts are limited and will bring patients The pain of secondary trauma; allograft bone grafts have a large immune rejection, causing virus transmission and infection, and there are potential safety hazards

Method used

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  • Composite artificial bone for enhancing osteogenic activity and preparation method thereof
  • Composite artificial bone for enhancing osteogenic activity and preparation method thereof
  • Composite artificial bone for enhancing osteogenic activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Step 1: Prepare a 10% collagen aqueous solution with deionized water.

[0042] Collagen is animal-derived full-length collagen, collagen polypeptide, and gelatin.

[0043] Step 2: Add nano-hydroxyapatite powder into the collagen aqueous solution in batches, the mass ratio of collagen to hydroxyapatite is 1:2, and mix well on a vortex shaker at room temperature.

[0044] Step three: quickly transfer the mixture obtained in step three to the mold.

[0045] Step 4: Pre-freeze the mold at 4°C for 20 minutes, then move to -20°C for 1 hour, transfer to -80°C for 3 hours, and then dry in a vacuum freeze dryer for 12 hours.

[0046] Step 5: prepare a 2% olive polyphenol solution with an ethanol solution, and adjust the pH to 6.

[0047] The ethanol solution is an aqueous ethanol solution with a mass fraction of 75%; the pH regulator is selected from sodium hydroxide solution or hydrochloric acid solution, and the molar volume concentration is 0.1mol / L.

[0048] Step 6: Incub...

Embodiment 2

[0051] Step 1: Prepare a 15% collagen aqueous solution with deionized water.

[0052] Collagen is recombinant collagen or human-like collagen produced by genetic engineering.

[0053]Step 2: Add nano-hydroxyapatite powder into the collagen aqueous solution in batches, the mass ratio of collagen to hydroxyapatite is 1:3, and mix well on a vortex shaker at room temperature.

[0054] Step three: quickly transfer the mixture obtained in step three to the mold.

[0055] Step 4: Pre-freeze the mold at 4°C for 40 minutes, then move to -20°C for 1.5 hours, transfer to -80°C for 4.5 hours, and then dry in a vacuum freeze dryer for 30 hours.

[0056] Step 5: Prepare a 3% olive polyphenol solution with an ethanol solution, and adjust the pH to 7.

[0057] The ethanol solution is an aqueous ethanol solution with a mass fraction of 85%; the pH regulator is potassium hydroxide solution and sulfuric acid solution, both of which have a molar volume concentration of 0.5 mol / L.

[0058] Step...

Embodiment 3

[0061] Step 1: prepare a collagen aqueous solution with a mass fraction of 25% with deionized water.

[0062] Collagen is recombinant collagen or human-like collagen produced by genetic engineering.

[0063] Step 2: Add the nano-hydroxyapatite powder into the collagen aqueous solution in batches, the mass ratio of collagen to hydroxyapatite is 1:4, and mix well on a vortex shaker at room temperature.

[0064] Step three: quickly transfer the mixture obtained in step three to the mold.

[0065] Step 4: Pre-freeze the mold at 4°C for 60 minutes, then move to -20°C for 2 hours, transfer to -80°C for 6 hours, and then dry in a vacuum freeze dryer for 48 hours.

[0066] Step 5: Prepare a 5% olive polyphenol solution with an ethanol solution, and adjust the pH to 8.

[0067] The ethanol solution is an aqueous ethanol solution with a mass fraction of 95%; the pH regulator is sodium carbonate solution or phosphoric acid solution, and the molar volume concentration is 1mol / L.

[006...

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Abstract

The invention relates to a composite artificial bone for enhancing osteogenic activity and a preparation method thereof. A human bone implant material needs to supply a certain compression resistance support at the implanted part to maintain and supply a spatial structure for cells to grow and shin, and in addition, a minienvironment similar to the environment of a natural bone needs to be provided so as to facilitate adhesion, proliferation and differentiation of cells, induce ingrowth of vessels, and finally to mediate generation of new bones. The preparation method of the composite artificial bone comprises the following steps: preparing a collagen aqueous solution, adding nano-hydroxyapatite, fully and uniformly mixing, pouring into a mold, prefreezing at 4 DEG C, -20 DEG C and -80 DEG C respectively, performing vacuum freeze drying, placing in a polyphenol solution for incubation, prefreezing at 4 DEG C, -20 DEG C and -80 DEG C respectively once again, and performing vacuum freeze drying. According to the composite artificial bone and the preparation method thereof disclosed by the invention, oleuropein is used for crosslinking, so the mechanical properties of stent materials are improved by virtue of bonding effects, and the osteogenic activity of the artificial bone stent materials are greatly improved by virtue of good antioxidant activity and osteogenic promotion property of the oleuropein.

Description

technical field [0001] The invention relates to a biomedical material, in particular to a composite artificial bone with enhanced osteogenic activity and a preparation method thereof. Background technique [0002] Bone defects due to osteoarthritis, femoral head necrosis, trauma, infection, tumor resection, etc., as well as in plastic surgery, maxillofacial repair and other operations, the demand for bone scaffold materials is increasing. Commonly used autologous bone and allogeneic bone grafts have deficiencies to varying degrees. For example, although autologous bone grafts have no immune rejection and have good osteoinductivity, the sources of autologous bone grafts are limited and will bring patients The pain of the secondary trauma; the allograft bone graft has a large immune rejection, which causes virus transmission and infection, and there are potential safety hazards. It has become a trend to study and synthesize artificial bone scaffold materials with excellent b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/42A61L27/54
Inventor 范代娣惠俊峰马晓轩米钰范慧
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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