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Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer

A bio-fertilizer, microorganism technology, applied in the direction of microorganism-based methods, chemicals for biological control, microorganisms, etc., to achieve the effect of increasing yield and reducing morbidity

Inactive Publication Date: 2015-07-08
SHANDONG PUFANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the bio-fertilizer containing the above-mentioned microorganisms cannot effectively control the pathogenic bacteria of the crops while increasing the crop yield.

Method used

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  • Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer
  • Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer
  • Microbe, application of microbe in bio-fertilizer preparation, bio-fertilizer and application of bio-fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the screening of this microorganism

[0032] 1. Materials

[0033] Soil for testing: From the same continuous-cropping cucumber greenhouse in Shouguang City, Shandong Province, remove the topsoil and collect 3-15cm rhizosphere soil, about 100g per sample. The soil samples were numbered, bagged, and brought back to the laboratory for storage in a 4°C refrigerator.

[0034] Test medium: PDA medium, Bengal red medium.

[0035] 2. Preparation of soil suspension

[0036] Put the soil sample into a sterilized mortar and grind it in a sterile operating bench until the particles are uniform. Using the 10-fold ratio dilution method, first weigh 1g of the ground soil, put it into a triangular flask filled with 99mL of sterile water and shake it for 30min to make a 10-2 soil dilution, then take 1mL and add it to 9mL of sterile water. Fully oscillate in water to make a 10-3 dilution, and so on, make a 10-4 dilution for later use.

[0037] 3. Plate inoculation

...

Embodiment 2

[0047] Embodiment 2, the inhibitory action of this microorganism to crop pathogen

[0048] Pathogenic bacteria for testing: Fusarium oxysporum transformation type of cucumber, Botrytis cinerea, Verticillium dahliae, Cucumber wilt fungus, Rhizoctonia solani (among them, Fusarium oxysporum transformation type of cucumber can cause cucumber wilt, Botrytis cinerea Botrytis cinerea causes foliage, Verticillium dahliae causes verticillium wilt, and Rhizoctonia solani causes sheath blight).

[0049] Test medium: PDA medium, PDB medium, Bengal red medium.

[0050] In the examples of the present invention, the microbial growth rate method was used to measure the antibacterial activity of the aseptic fermentation filtrate of the microorganisms.

[0051] The microorganisms preserved above were inoculated into PDB medium, cultured at 25° C. and 160 r / min for 8 days, and then filtered through a 0.45 μm microporous membrane to obtain a sterile fermentation filtrate. Mix the filtrate with ...

Embodiment 3

[0056] Embodiment 3, the preparation of this microbial spore suspension

[0057] Specifically prepare as follows:

[0058] Transfer the refrigerated microbial strains to the PDA medium plate (200g potato, 20g sucrose, 18g agar, 1000mL distilled water) for 3 generations; then use an inoculation loop to pick the edge hyphae of the 3rd generation colony, and transfer to PDB In the liquid culture medium (200g of potatoes, 20g of sucrose, 1000mL of distilled water), at 25°C to 30°C, the optimum temperature is 28°C, 120rpm, cultivated for 10 days under the conditions to obtain the fermentation liquid of the microorganism. The fermented liquid obtained was filtered through a 100 μm sieve to obtain a spore suspension, and the live spore concentration was 3.0×10 10 -5.0×10 10 cfu / ml.

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Abstract

The embodiment of the invention provides a microbe, an application of the microbe in bio-fertilizer preparation, a bio-fertilizer and an application of the bio-fertilizer. Compared with the existing microbe for the bio-fertilizer and the existing bio-fertilizer in the prior art, the microbe provided by the invention is rhizopus stolonifer PF102, so that the microbe can be used for preparation of the bio-fertilizer; when the bio-fertilizer used for crop cultivation, the bio-fertilizer can improve the output of the crops, greatly reduce the morbidity of the crops and death rate due to disease, and in the meantime improve the growth conditions of the crops such as microbial population at the crop rhizosphere, soil bulk density, soil porosity, length and width of the plant leaf, and fruit quantity.

Description

technical field [0001] The invention relates to the field of biological fertilizers, in particular to microorganisms and their application in preparing biological fertilizers, biological fertilizers and their applications. Background technique [0002] In recent years, with the strengthening of people's awareness of environmental protection and the improvement of quality of life requirements, bio-fertilizer is a green and efficient fertilizer because it will not pollute the environment. It has been rapidly developed and widely used in crop cultivation. [0003] In related technologies, microorganisms commonly used in biological fertilizers include nitrogen-fixing bacteria: such as Rhizobium; phosphate-dissolving bacteria: such as Bacillus megaterium, Bacillus licheniformis, Bacillus cereus, and Bacillus subtilis; potassium-solubilizing bacteria: such as Bacillus colloid. [0004] However, the biological fertilizer containing the above-mentioned microorganisms cannot effectiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G3/00A01P1/00A01P3/00A01P21/00C12R1/845
Inventor 杨文慧
Owner SHANDONG PUFANG BIOTECH
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