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Silk fibroin membrane and preparation method thereof

A silk fibroin and silk fibroin technology, applied in catheters, medical science, prostheses, etc., can solve the problems of damage to nanostructures, poor mechanical properties of silk fibroin nanofiber membranes, etc., and achieve high dissolved concentration and excellent biocompatibility. and the effect of degradation performance, excellent mechanical properties

Active Publication Date: 2013-10-09
苏州盛泽科技创业园发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the other hand, inside the natural silk, there is a wire structure with a diameter of tens of nanometers in the silk gland before silkworm spinning, and this nanostructure plays a key role in the formation of silk and the excellent mechanical properties of silk; however, the existing technology In the natural silk regeneration process, the dissolution step severely damaged the nanostructure, which became one of the main reasons for the poor mechanical properties of the regenerated silk fibroin nanofiber membranes.

Method used

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  • Silk fibroin membrane and preparation method thereof
  • Silk fibroin membrane and preparation method thereof
  • Silk fibroin membrane and preparation method thereof

Examples

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Effect test

Embodiment 1

[0034] (1) Natural mulberry silk was boiled with 0.5wt% sodium carbonate solution for 30 minutes, degummed, and degummed mulberry silk was obtained after repeating 3 times;

[0035] (2) Place degummed mulberry silk in 80wt% formic acid containing 2wt% calcium chloride at a concentration of 15wt%, stir and dissolve at room temperature for 2 hours, and then pour it into a polyethylene plate to dry to form a film;

[0036] (3) Soak the film in step (2) in running water for 8 hours to remove calcium chloride, then place it in a vacuum drying oven at 40°C, and dry it for 24 hours to obtain a silk fibroin film.

[0037] attached figure 1 , figure 2 The scanning electron microscope images of the surface and section of the above silk fibroin film are respectively; it can be seen that the surface of the silk fibroin film prepared by the present invention is smooth and smooth, and a large number of nanowire structures can be seen in the section.

Embodiment 2

[0039] (1) Natural mulberry silk was degummed by boiling 0.5wt% sodium carbonate solution for 30 minutes, and degummed mulberry silk was obtained after repeating 3 times;

[0040] (2) Place degummed mulberry silk in 95wt% formic acid containing 5wt% lithium bromide at a concentration of 20wt%, stir and dissolve at room temperature for 6 hours, and then pour it into a polyethylene plate to dry to form a film;

[0041] (3) Soak the membrane in step (2) in running water for 10 hours to remove lithium bromide, and then dry it in a vacuum drying oven;

[0042] (4) Dissolve the film in step (3) in 80wt% formic acid to prepare a 20wt% silk fibroin solution, then pour it into a polyethylene plate and dry it to form a silk fibroin film;

[0043] (5) Soak the silk fibroin film obtained in step (4) in flowing water for 1 hour to remove residual formic acid, and then vacuum-dry it at 60° C. for 20 hours to obtain a silk fibroin film.

Embodiment 3

[0045] (1) Natural mulberry silk was degummed by boiling 0.5wt% sodium carbonate solution for 30 minutes, and degummed mulberry silk was obtained after repeating 3 times;

[0046] (2) Put the degummed mulberry silk at a concentration of 10wt% in 50wt% mixed acid containing 5wt% lithium thiocyanate (phosphoric acid: formic acid = 1:4), stir and dissolve at 60°C for 1 hour, and then pour it into a polyethylene plate dry film;

[0047] (3) Soak the silk fibroin film in step (2) in running water for 1 hour to remove salt and residual acid, and then dry it in a vacuum drying oven;

[0048] (4) Dissolve the film in step (3) in hexafluoroisopropanol to prepare a 10wt% solution, and dissolve chitosan in hexafluoroisopropanol to prepare a 10wt% solution, and then mix the two solutions by 1: 1 volume blend preparation concentration is 10wt%, silk fibroin and polylactic acid 1:1 blended silk fibroin solution;

[0049](5) Pour the silk fibroin / chitosan blended silk solution prepared in ...

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Abstract

The invention discloses a silk fibroin membrane and a preparation method of the silk fibroin membrane. The preparation method comprises the following specific steps of: dissolving natural silk taken as a main raw material in an acid inorganic salt solution, forming a membrane, desalting, and carrying out vacuum drying to prepare the silk fibroin membrane which is insoluble in water and has high mechanical property. The silk fibroin membrane has the breaking strength of greater than 50Mpa at dry state, the breaking elongation of greater than 5% at dry state, the breaking strength of greater than 10Mpa at wet state and the breaking elongation of greater than 150% at wet state. In addition, the silk fibroin membrane prepared by the method is stable and controllable in structure, has good biocompatibility and can serve as a medicinal biological material. The preparation method disclosed by the invention is simple and short in flow path, has high film formation efficiency and is suitable for industrialization large-scale production.

Description

technical field [0001] The invention relates to a polymer film and a preparation method thereof, in particular to a silk fibroin film and a preparation method thereof. The method is simple and efficient and can be widely used in the fields of biology and medicine. Background technique [0002] my country is the main producer of silk, and its silk output accounts for more than 70% of the world's output. In recent years, the research and application of silk has extended from the traditional textile field to high-tech fields, such as optoelectronics and biomedical materials, especially as biomedical materials have made important progress. Natural silk is fibrous, and biomedical materials require diversity and plasticity in the shape of scaffolds. Therefore, the regeneration and processing of natural silk fibers is a necessary step to broaden its application range. At present, silk protein can be processed into ropes, regrowth silk, nanofiber membranes, silk fibroin membranes, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/22A61L29/04A61L31/04
Inventor 左保齐吕强张锋
Owner 苏州盛泽科技创业园发展有限公司
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