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Method for producing 2,3-butanediol fermentation broth from wheat bran

A technology of wheat bran and butanediol, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high raw material cost, high glucose price, complex fermentation liquid components, etc., and reduce fermentation inhibition The formation of substances, reducing the loss of pentosan, and improving the effect of comprehensive utilization

Inactive Publication Date: 2014-03-26
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of 2,3-butanediol by biotransformation mostly uses glucose as a carbon source, but the price of glucose is relatively high, and there are problems of competing with people for food and land
In addition, due to the strong hydrophilicity and high boiling point of 2,3-butanediol, and the complex composition of the fermentation broth, the downstream separation of this product is relatively difficult
High raw material cost and difficult product separation have become the bottleneck of large-scale industrial production of 2,3-butanediol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The specific steps of using wheat bran to produce 2,3-butanediol fermentation broth are as follows:

[0022] Remove impurities from the wheat bran, dry, pulverize with a pulverizer, pass a 20-mesh sieve to obtain wheat bran starch, and collect light brown wheat husks that have not passed the 20-mesh sieve to obtain wheat bran husks;

[0023] Stir 200 g wheat bran starch, 500 g water, 3 g α-amylase, 280 rpm for 3 minutes, keep the temperature at 70°C for 20 minutes, heat up to 90°C, keep the temperature for 12 hours, filter, collect the supernatant, and obtain wheat Bran starch sugar;

[0024] Crush the wheat bran husk and pass through a 20-mesh sieve to obtain wheat bran husk powder. Mix 120 g of wheat bran husk powder and 450 mL sulfuric acid with a concentration of 1%, keep it at 121°C for 1 hour, filter, and use a concentration of 20%. Adjust the pH of sodium hydroxide to neutral to obtain wheat bran hemicellulose sugar; wash the residue with water to neutral pH to obtain ...

Embodiment 2

[0030] The specific steps of using wheat bran to produce 2,3-butanediol fermentation broth are as follows:

[0031] Remove impurities from the wheat bran, dry, pulverize with a pulverizer, and pass a 20-mesh sieve to obtain wheat bran starch, and collect the light brown wheat husks that have not passed the 20-mesh sieve to obtain wheat bran husks;

[0032] Stir 300 g wheat bran starch, 550 g water, 6 g α-amylase, 300 rpm for 5 minutes, keep the temperature at 80°C for 25 minutes, heat up to 92°C for 12 hours, filter and collect the supernatant to obtain Wheat bran starch sugar;

[0033] Crush the wheat bran husk and pass through a 20-mesh sieve to obtain wheat bran husk powder. Mix 120 g of wheat bran husk powder and 450 mL sulfuric acid with a concentration of 1%, keep it at 121°C for 1 hour, filter, and use a concentration of 20%. Adjust the pH of the sodium hydroxide to neutral to obtain wheat bran hemicellulose sugar; wash the residue with water to neutral pH to obtain wheat bra...

Embodiment 3

[0039] The specific steps of using wheat bran to produce 2,3-butanediol fermentation broth are as follows:

[0040] Remove impurities from the wheat bran, dry, pulverize with a pulverizer, and pass a 20-mesh sieve to obtain wheat bran starch, and collect the light brown wheat husks that have not passed the 20-mesh sieve to obtain wheat bran husks;

[0041] Stir 250 g wheat bran starch, 520 g water, 4 g α-amylase, 290 rpm for 4 minutes, keep the temperature at 75°C for 22 minutes, raise the temperature to 90°C, keep the temperature for 11 hours, filter, collect the supernatant, and obtain wheat Bran starch sugar;

[0042] Crush the wheat bran husks and pass through a 20-mesh sieve to obtain wheat bran husk powder. Mix 100 g of wheat bran husk powder and 480 mL sulfuric acid with a concentration of 1.5%. Incubate at 121°C for 1 hour, filter, and use a concentration of 20%. Adjust the pH of the sodium hydroxide to neutral to obtain wheat bran hemicellulose sugar; wash the residue with ...

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Abstract

The invention relates to a method for producing 2,3-butanediol fermentation broth from wheat bran. The method concretely comprises the following two steps of: 1. preparing wheat bran sugar from the wheat bran; and 2. mixing and fermenting Klebsiella freeze-dried powder, the wheat bran sugar and fermentation nutritional factors, so as to obtain fermentation broth with 85.5-90.2g / L of 2,3-butanediol and 21-35g / L of acetoin, wherein the yield of 2,3-butanediol is 0.21-0.25g of 2,3-butanediol per g of the wheat bran sugar, and the production intensity of the 2,3-butanediol is 2.9-3.5g / L / h. The fermentation efficiency of the method is approximate to that of mixed sugar of glucose and xylose, and the material cost is far lower than the cost of the glucose and the xylose. According to the method, starch is hydrolyzed by alpha-amylase, cellulose and pectin are hydrolyzed by cellulase and pectinase, the hydrolysis efficiency is improved remarkably, the high-quality wheat bran sugar is obtained with low cost, and the comprehensive utilization rate of the wheat bran sugar is increased.

Description

Technical field [0001] The invention belongs to the field of comprehensive utilization of byproducts of agricultural product processing, and specifically is a method for converting wheat bran, which is a byproduct of wheat flour processing, into 2,3-butanediol through fermentation technology. Background technique [0002] 2,3-Butanediol is also called 2,3-bishydroxybutane or dimethylene glycol. 2,3-Butanediol can be used as a potential platform compound to replace the traditional platform compound-carbon tetrahydrocarbon , Used for large-scale synthesis of good solvent methyl ethyl ketone, and widely used in 1,3-butadiene in synthetic rubber, polyester and polyurethane fields. In addition, 2,3-butanediol can also be used to prepare inks, perfumes, fumigants, moisturizers, softeners, plasticizers, explosives, pharmaceutical chiral carriers, food flavors and fuel additives. At present, most of the production of 2,3-butanediol by biotransformation uses glucose as a carbon source, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12P7/26C12R1/22
Inventor 杨培周姜绍通罗水忠郑志潘丽军
Owner HEFEI UNIV OF TECH
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