Improved method for extracting berberine

Abstract

The invention belongs to the field of extraction of plant active ingredients and relates to an extraction purifying method for removing plant cell walls with cellulase and breaking cells accordingly, in particular to an improved method for extracting berberine. The improved method includes crushing golden cypress, washing and steaming, subjecting the crushed golden cypress to reaction with enzyme, digesting by lime milk, depositing and crystallizing, drying and the like. The improved method for extracting berberine is high in digestion rate, short in extraction period, and low in solvent usage. With degradation of cell walls by cellulase, the cell walls are broken in different degrees, permeability of cells is improved, digestion rate of active components in the cells is increased, extraction time is shortened, and further, usage of the solvent is lowered, and production efficiency is improved. Besides, the method is energy-saving, environment-friendly and safe. Enzyme reaction is mild in conditions, requirements for engineering equipment are not high, and operation is convenient; materials required by the enzyme reaction causes no pressure to the environment, energy consumption and pollution to the environment are reduced.

Description

[0001] technical field [0002] The invention belongs to the field of extraction of plant active ingredients, and relates to an extraction and purification method for removing plant cell walls by using cellulase to break cells, in particular to an improved method for extracting berberine. [0003] Background technique [0004] Pampas ( Phellodendron chinense Schneid . ) for Rutaceae ( Rutaceae ) Phellodendron ( Phellodendron Rupr . ) plant. Phellodendron sichuanensis is the dry bark of the yellow bark tree. The chemical components of Cortex Phellodendri mainly include alkaloids, flavonoids, lactones, terpenes, sterols, and volatile oils, among which berberine in the original berberines of alkaloids is the main one. Main pharmacological effects: antibacterial, anti-inflammatory, lowering blood pressure, lowering blood sugar, anti-tumor, antitussive, expectorant, inhibiting stress ulcers, clearing away heat and dampness, purging fire and removing steam, detoxifying ...

AI Technical Summary

Problems solved by technology

The application number is 200810069671.8, the title "a kind of Coptis chinensis total alkaloid extraction process" and the application number is 200910191620.7, and the title "the extraction method of Coptis alkaloids" all uses the acid extraction method, but because Phellodendron Phellodendri contains a large amount of mucus, it is difficult to operate It brings some inconvenience; although the ultrasonic method and the microwave method have broad prospects, they have higher requirements for containers and may destroy the characteristics of active ingredients, so they have higher requirements for engineering equipment and must be used on a large scale production, but also to further solve problems such as engineering equipment, such as application number 02103880.5, title "extraction process and new application of Coptis chinensis total alkaloids"; alcohol extraction and acid alcohol method are generally reflux extraction, although the extraction rate is high, but To consume a large amount of energy, increased production costs, as the application number is 200410065893.4, named "Coptidis Rhizoma, Evodia rutaecarpa total alkaloid extraction method and preparation thereof"

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