Colloidal gold immune test paper strip for cubilose and preparation method for colloidal gold immune test paper strip

Abstract

The invention provides a colloidal gold immune test paper strip for cubilose. The colloidal gold immune test paper strip for cubilose comprises a sample pad, a combination pad, a reaction pad, a water absorption pad, and a polyvinyl chloride (PVC) backing; the sample pad, the combination pad, the reaction pad and the water absorption pad are sequentially adhered to the PVC backing; the combination pad is adhered to the sample pad and is overlapped with the reaction pad; the reaction pad is overlapped with the water absorption pad; the combination pad is coated with an anti-sialoglycoprotein antibody colloidal gold marker; and the reaction pad is coated with a detection line consisting of sialoglycoprotein antigen and a quality control line consisting of goat anti mouse immunoglobulin G (IgG). The cubilose and relative products thereof can be qualitatively detected by using the colloidal gold immune test paper strip; the colloidal gold immune test paper strip for cubilose has a wide application range, is high in specificity, and has a good effect of matrix interference resistance, and is low in detection cost; and by using the colloidal gold immune test paper strip, the cubilose and relative products thereof can be quickly detected on the scene.

Description

technical field [0001] The invention relates to the detection of bird's nest and related products, in particular to a bird's nest colloidal gold immune test strip and a preparation method thereof. Background technique [0002] Bird's nest is a nest formed by the condensation of saliva or down feathers of swiftlets of the Swiftlet family Swiftlet and similar species. Its origin is distributed in Southeast Asia, such as China, Indonesia, Thailand, Vietnam and Malaysia. Bird's nest is a kind of precious traditional Chinese medicine, which has the functions of nourishing yin and moistening dryness, nourishing the middle and nourishing qi, nourishing the stomach and spleen, resolving phlegm and relieving cough; bird's nest is rich in active glycoprotein, calcium, iron, phosphorus, iodine and vitamins and other natural nutrients And minerals, it also has the effect of preventing influenza virus, promoting cell growth and strengthening the function of the immune system. Due to the...

AI Technical Summary

Problems solved by technology

[0004] (1) PCR method: use specific gene fragments in bird's nest for PCR detection. This method has good specificity, but the detection time is long, requires expensive equipment and professional operators, and the finished product of bird's nest often requires thermal processing, which is easy to cause sample loss. DNA degradation in the medium, resulting in false negative test results;

[0005] (2) Electrophoresis method: analysis is based on the protein map of bird’s nest, but the ability to resist matrix interference is poor and the sensitivity is low. It is limited to the detection of bird’s nest and cannot be used for the detection of bird’s nest products;

[0006] (3) Specific component analysis method: use chromatography, spectroscopy and other means to identify the authenticity of various chemical components in bird’s nest, such as specific elements, amino acids, sialic acid, sugars, etc., but its specificity is not high , easily disturbed by artificial additives

[0007] Therefore, the existing detection technology has low specificity, is easily interfered by artificial additives, and has a narrow scope of application. The detection operation is cumbersome, the detection time is long, it needs to be equipped with large analytical instruments and requires professional operators, etc.

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