Bird's nest colloidal gold immune test strip and preparation method thereof

Abstract

The invention provides a bird's nest colloidal gold immune test strip, comprising a sample pad, a binding pad, a reaction pad, a water-absorbing pad and a PVC backing, and the PVC backing is sequentially adhered to a sample pad, a binding pad, a reaction pad and a water-absorbing pad; the combination pad is adhered to the sample pad and overlapped with the reaction pad; the reaction pad is overlapped with the absorbent pad; the combination pad is coated with antisialosaccharide Protein antibody colloidal gold markers, the reaction pads are respectively coated with a detection line composed of sialoglycoprotein antigen and a quality control line composed of goat anti-mouse IgG. The invention can perform qualitative detection on the bird's nest and its related products, has wide application range, good specificity, good anti-matrix interference effect, can realize on-site rapid identification of the bird's nest and its related products, and has low detection cost.

Description

technical field [0001] The invention relates to the detection of bird's nest and related products, in particular to a bird's nest colloidal gold immune test strip and a preparation method thereof. Background technique [0002] Bird's nest is a nest formed by the condensation of saliva or down feathers of swiftlets of the Swiftlet family Swiftlet and similar species. Its origin is distributed in Southeast Asia, such as China, Indonesia, Thailand, Vietnam and Malaysia. Bird's nest is a kind of precious traditional Chinese medicine, which has the functions of nourishing yin and moistening dryness, nourishing the middle and nourishing qi, nourishing the stomach and spleen, resolving phlegm and relieving cough; bird's nest is rich in active glycoprotein, calcium, iron, phosphorus, iodine and vitamins and other natural nutrients And minerals, it also has the effect of preventing influenza virus, promoting cell growth and strengthening the function of the immune system. Due to the...

AI Technical Summary

Problems solved by technology

[0004] (1) PCR method: use specific gene fragments in bird's nest for PCR detection. This method has good specificity, but the detection time is long, requires expensive equipment and professional operators, and the finished product of bird's nest often requires thermal processing, which is easy to cause sample loss. DNA degradation in the medium, resulting in false negative test results;

[0005] (2) Electrophoresis method: analysis is based on the protein map of bird’s nest, but the ability to resist matrix interference is poor and the sensitivity is low. It is limited to the detection of bird’s nest and cannot be used for the detection of bird’s nest products;

[0006] (3) Specific component analysis method: use chromatography, spectroscopy and other means to identify the authenticity of various chemical components in bird’s nest, such as specific elements, amino acids, sialic acid, sugars, etc., but its specificity is not high , easily disturbed by artificial additives

[0007] Therefore, the existing detection technology has low specificity, is easily interfered by artificial additives, and has a narrow scope of application. The detection operation is cumbersome, the detection time is long, it needs to be equipped with large analytical instruments and requires professional operators, etc.

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