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Streptomyces strain for inhibiting harm of Phytophthora parasitica var. nicotianae and application thereof

A technology of tobacco black shank bacteria and tobacco black shank, which is applied in the field of preventing tobacco black shank from harming tobacco, and achieves the effect of improving yield and quality

Inactive Publication Date: 2011-11-23
周倩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The principle of bio-control bacteria is to directly antagonize the pathogen of tobacco black shank. Some bio-control bacteria can also induce tobacco to develop resistance to black shank. If the biological agent is applied in a timely manner, it can play a long-term role in the prevention and control of the disease. Although the application of biocontrol bacteria in biological control has just started, some good progress has been made

Method used

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  • Streptomyces strain for inhibiting harm of Phytophthora parasitica var. nicotianae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Screening of Streptomyces strain D35

[0038] 72 soil samples were collected from Hupingshan, Huashan, Jiuzhaigou and other national nature reserves in Hunan, and were screened with Gaoshi No. 1 medium, and 428 strains were screened, and plate antagonism experiments were carried out one by one, and the black shank pathogen inhibitory strains were obtained. Conduct bioassay experiments. The isolated and purified strain D35 was inoculated in Gaoshi No. 1 liquid medium. Shake flask fermentation at 28°C for 60 hours, and filter the fermentation broth with a bacterial filter. Add 3 mL of filtrate to each sterile petri dish, pour into PDA medium (about 55°C), shake well, and make a plate. Divide into small bacterial blocks of 4-5 mm, and transfer them upside down to the center of the plate. Repeat 3 times. After standing for 1 hour, put it into an incubator, and incubate at 28°C for 5 days. The diameter of the bacterial block was measured by the cross method, ...

Embodiment 2

[0039] Example 2: Physiological and biochemical characteristics of Streptomyces strain D35 and its preservation

[0040] The screened bacterial strains were deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on August 27, 2009, with the preservation number: CGMCCNo.3250.

[0041] Physiological characteristics of the strain:

[0042] Strain D35 has the typical characteristics of Streptomyces. It grows on Gao's No. 1 medium, and the colonies are compact, wrinkled, rugged, crusty, and purple-brown. Both the aerial mycelia and the hyphae in the base are very abundant. The aerial mycelium forms spore chains, and the spores are purple-gray, and grow in Gaoshi No. 1 liquid medium to produce brown pigments. D35 strain can utilize L-arabinose, D-arabinose, glucose, fructose, sucrose, rhamnose, raffinose, mannose and maltose. The strain cannot liquefy gelatin, hydrolyze starch, or decompose cellulose, but can produce hydrogen sulfide....

Embodiment 3

[0045] Embodiment 3: the preparation of Streptomyces strain D35 fermented liquid

[0046] (1) Cultivation of strains

[0047] Prepare Gao's No. 1 slant medium, inoculate it, put it in a 28°C incubator and cultivate it for 72 hours, and store it for later use.

[0048] (2) Preparation of fermentation broth: Prepare shake flask culture medium (Gaoshi No. 1 culture fluid), pack into triangular flasks (500mL bottle, 100mL liquid) before sterilization, routinely sterilize, and put it on the ultra-clean workbench after cooling , directly wash the slanted bacteria with water and put them into a fermentation Erlenmeyer flask, shake the flask for cultivation, 28°C, 200r / min, and cultivate for 48h. Immediately transfer to fermenter fermentation, refer to the preparation of fermenter medium (recipe: soluble starch 30g, K 2 HPO 4 0.5g, KNO 3 1g, FeSO 4 ·7H 2 O 0.01g, NaCl 0.5g, MgSO 4 0.5g, peptone 10g, distilled water 1000mL, pH7.2.), sterilize, inoculate and ferment after cooling...

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Abstract

Aiming at the existing defects in preventing and controlling black shank of tobacco, the invention provides Streptomyces misawanensis D35 for inhibiting Phytophthora parasitica var. nicotianae. The strain was collected in China General Microbiological Culture Collection Centre (CGMCC) on August 27, 2009 with the collection number of CGMCC No: 3250. The invention also provides application of the strain in effectively inhibiting diffusion of Phytophthora parasitica var. nicotianae in tobacco, reducing disease incidence, improving the tobacco yield and quality, facilitating the development of biological prevention and control products and further promoting the safe control of Phytophthora parasitica var. nicotianae.

Description

technical field [0001] The invention relates to a strain of streptomyces and its use for inhibiting the harm of tobacco black shank. More specifically, the invention provides a preserved strain for inhibiting the harm of tobacco black shank, which can greatly reduce the harm of tobacco black shank. The invention belongs to the technical field of preventing and controlling tobacco black shank damage to tobacco. Background technique [0002] Tobacco black shank (Phytophthora parasitica var.nicotiane) is a type of oomycete disease discovered by van Breda de Haan on tobacco in Java, Indonesia in 1896. It mainly infects tobacco roots and stem bases. The disease is a devastating disease of tobacco, and it is also one of the most serious diseases in the world's tobacco production. In 1922, it became a serious disease in the cigar smoking areas of Florida and Kansas, USA. After 1924, the disease has spread all over the tobacco fields in temperate, subtropical and tropical regions ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04A01N63/02A01P3/00C12R1/465
Inventor 周倩
Owner 周倩
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