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Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof

A detection method, the technology of aquinone, which is applied in the HPCE detection of alopecia, aquinone and alopecia, in the field of alopecia B, and achieves the effects of shortened analysis time, good correlation, and reduced environmental pollution

Inactive Publication Date: 2011-06-15
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

Because there are many inconveniences in the analysis of the above four kinds of fennel coumarins by HPLC, the present invention provides a rapid method for detecting four active components in the fennel medicinal material or its extract by using HPCE, and provides a new means for the quality control of fennel

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  • Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof
  • Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof
  • Method for detecting esculin, aesculetin, fraxin and fraxetin in cortex fraxini or extract thereof

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[0030] 1 Instruments, reagents and samples

[0031] American Agilent 3DCE capillary electrophoresis instrument (model: G1600A, including diode array detector and HP chromatographic workstation); fused silica capillary effective length 45cm (53.5cm×75μm ID, Hebei Yongnian Ruifeng Chromatography Device Co., Ltd.); American Waters 515 Waters 486 detector; 7725i manual injector; pre-column: Phenomenex Security Guard; ChromTek chromatography workstation (American Sage Inc.).

[0032] Microfuge 18 desktop centrifuge (Beckman Coulter Co., Ltd., USA); Boda 1004 ultrasonic cleaner (Shenzhen Boda Ultrasonic Engineering Equipment Co., Ltd.); TGL.16G desktop high-speed centrifuge (Shanghai Medical Analytical Instrument Factory); BP210D One-hundred-thousandth electronic analytical balance (Sartorius, Germany); Milli-Q ultrapure water device (Millipore, USA); filter device and microporous membrane (0.45 μm).

[0033]The content determination of fencetin A (110740-200104), fencetrin (111727...

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Abstract

The invention discloses a high performance capillary electrophoresis (HPCE) quick quantitative method for four active components of fraxin, esculin, fraxetin and aesculetin in cortex fraxini or extract thereof. By the method provided by the invention, four cortex fraxini coumarins are well separated and determined in 10min, and simultaneously all the components have good linear relations and highrecovery rates. The method provided by the invention has good relativity of determination results compared with a high performance liquid chromatography (HPLC) method, greatly shortens the analyzing time, obviously reduces environmental pollution, notably decreases the analyzing and operating cost, provides a quick efficient analyzing means for researching the distribution of the active components of cortex fraxini and the variation rule of content in different harvest phases, and also can be used for researching the inherent quality of the cortex fraxini extract and for referencing in development research of new drugs.

Description

technical field [0001] The invention relates to a detection method for active components in traditional Chinese medicines, in particular to an HPCE detection method for fencetrin, fencetine B, fencetrin and fencetrin in medicinal materials or extracts thereof. Background technique [0002] Chrysanthemum chinensis varies from place to place, and the sources of varieties are different, but they all belong to the genus Fraxinus of the Oleaceae family (Oleaceae), and their morphological characteristics and odor are similar to those recorded in Materia Medica. "Pharmacopoeia of the People's Republic of China" 2010 edition stipulates that Bark Bark is the dry bark of Fraxinus rhynchophylla Hance, Fraxinus chinensis Roxb., Fraxinus szabaona Lingelsh. or Fraxinus stylosa Lingelsh. or dry skin. Among them, the bitter ash tree is distributed in Jilin, Liaoning, Hebei, Henan and other places. Ash trees are distributed in North China, Northwest China, East China, Central South, Southw...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/63G01N27/447A61P29/00A61P1/12A61P15/00A61P27/02A61P31/00A61P7/10A61P7/02A61P37/08A61P11/14A61P11/10A61P11/06A61P25/28A61P35/00A61P31/04A61P11/00A61K129/00
Inventor 张琦
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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